TEL: +86 571 56623320 EMAIL: [email protected]
Product Name XAF1 Chinese Name XIAP相关因子1凋亡抑制蛋白抗体 Alias XIAP-associated factor 1; BIRC 4 binding protein; BIRC4-binding protein; XAF1; XAF1_MOUSE; XIAPAF1; BIRC 4 binding protein; BIRC4 binding protein; BIRC4BP; HSXIAPAF 1; HSXIAPAF1; XAF 1; XIAP associated factor 1. Research Area Cell biology immunology Apoptosis Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, Rat, Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 31kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from mouse XAF1: 151-250/273 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a protein which binds to and counteracts the inhibitory effect of a member of the IAP (inhibitor of apoptosis) protein family. IAP proteins bind to and inhibit caspases which are activated during apoptosis. The proportion of IAPs and proteins which interfere with their activity, such as the encoded protein, affect the progress of the apoptosis signaling pathway. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2012]
Function:
Seems to function as a negative regulator of members of the IAP (inhibitor of apoptosis protein) family. Inhibits anti-caspase activity of BIRC4. Induces cleavage and inactivation of BIRC4 independent of caspase activation. Mediates TNF-alpha-induced apoptosis and is involved in apoptosis in trophoblast cells. May inhibit BIRC4 indirectly by activating the mitochondrial apoptosis pathway. After translocation to mitochondra, promotes translocation of BAX to mitochondria and cytochrome c release from mitochondria. Seems to promote the redistribution of BIRC4 from the cytoplasm to the nucleus, probably independent of BIRC4 inactivation which seems to occur in the cytoplasm. The BIRC4-XAF1 complex mediates down-regulation of BIRC5/survivin; the process requires the E3 ligase activity of BIRC4. Seems to be involved in cellular sensitivity to the proapoptotic actions of TRAIL. May be a tumor suppressor by mediating apoptosis resistance of cancer cells (By similarity).
Subunit:
Interacts with BIRC1, BIRC2, BIRC3, BIRC4, BIRC7 and BIRC8. Part of an complex consisting of BIRC4, XAF1 and BIRC5; the complex formation requires IFN-beta stimulation (By similarity).
Subcellular Location:
Cytoplasm (By similarity). Nucleus (By similarity). Mitochondrion (By similarity).
Similarity:
Contains 1 TRAF-type zinc finger.
SWISS:
Q5NBU8
Gene ID:
327959
Database links:
- Entrez Gene: 54739 Human
- Entrez Gene: 327959 Mouse
- Entrez Gene: 679600 Rat
- Omim: 606717 Human
- SwissProt: Q6GPH4 Human
- SwissProt: Q5NBU8 Mouse
- Unigene: 441975 Human
- Unigene: 291131 Mouse
XAF1是一个新近发现的通过与XIAP直接结合拮抗其抗凋亡作用的蛋白因子。XAF1是一个新的Tumour抑制基因,XAF1在多种Tumour细胞和组织中存在低表达或表达缺失。其过度表达可诱导凋亡,抑制Tumour生长。Product Picture Sample:
Cerebellum(Rat) Lysate at 40 ug
Primary: Anti-FBXO39 (SL1308R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 50 kD
Tissue/cell: mouse stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-XAF1/FBXO39 Polyclonal Antibody, Unconjugated(SL1308R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-XAF1/FBXO39 Polyclonal Antibody, Unconjugated(SL1308R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Scan Wechat Qrcode