Product Name	SFRP1
Chinese Name	分泌型卷曲相关蛋白1抗体
Alias	Secreted frizzled related protein 1; Frizzled related protein 1; FRP 1; SARP 2; SARP-2; Secreted apoptosis related protein 2; Secreted Apoptosis-related Protein 2; Secreted frizzled related protein; Secreted Frizzled-related Protein 1; Secreted frizzled-related protein; SFRP 1; sFRP-1; SFRP1; SFRP1_HUMAN; SFRP1; FRP; FRP-1; FRP1; FrzA; SARP2.
literatures	Specific References  (3)     |     SL1303R has been referenced in 3 publications. [IF=2.942] Shi M et al. MicroRNA-27a targets Sfrp1 to induce renal fibrosis in diabetic nephropathy by activating Wnt/β-Catenin signalling. Biosci Rep. 2020 Jun 26;40(6):BSR20192794.  WB&ICF ;  Rat.   PubMed:32484208 [IF=1.52] Wang, Kun, et al. "MiR-27a regulates Wnt/beta-catenin signaling through targeting SFRP1 in glioma." NeuroReport 26.12 (2015): 695-702.  WB ;  Human.   PubMed:26164457 [IF=1.257] Hai Zhao. et al. Oxidative stress caused by a dysregulated Wnt/β-catenin signalling pathway is involved in abnormal placenta formation in pregnant mice with chronic fatigue syndrome. Zygote. 2020 Oct;:1-8  WB,IHC ;  Mouse.   PubMed:33054899
Research Area	Cell biology  Neurobiology  Signal transduction  Stem cells
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	33kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human SFRP1: 201-314/314
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling; epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer. This gene may also be involved in determining the polarity of photoreceptor cells in the retina. [provided by RefSeq, Sep 2009] Function: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription. Subunit: Interacts with WNT1, WNT2 and FRZD6. Interacts with WNT4 and WNT8. Subcellular Location: Secreted. Note=Cell membrane or extracellular matrix-associated. Released by heparin-binding. Tissue Specificity: Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes. Similarity: Belongs to the secreted frizzled-related protein (sFRP) family. Contains 1 FZ (frizzled) domain. Contains 1 NTR domain. SWISS: Q8N474 Gene ID: 6422 Database links: Entrez Gene: 6422 Human Entrez Gene: 20377 Mouse Omim: 604156 Human SwissProt: Q8N474 Human SwissProt: Q8C4U3 Mouse Unigene: 213424 Human Unigene: 281691 Mouse 分泌型卷曲相关蛋白1是近来发现的新的抑癌基因, 是Wnt信号通道的拮抗通路蛋白,SFRP1能够阻断某些发生在癌症初期与之相关的细胞生长基因的信号通路。 有学者认为：该基因有去甲基化酶的作用，可以阻断与甲基化进程相关酶的活性，进而抑制Tumour基因的改变，SFRP1的作用还有待于更进一步的研究。
Product Picture	Sample:Liver(Mouse) lysate at 30ug; Primary: Anti-SFRP1 (SL1303R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(SL0295G-HRP) at 1: 5000 dilution; Predicted band size : 33 kD Observed band size :33 kD Paraformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SFRP1) Polyclonal Antibody, Unconjugated (SL1303R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SFRP1) Polyclonal Antibody, Unconjugated (SL1303R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SFRP1) Polyclonal Antibody, Unconjugated (SL1303R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SFRP1) Polyclonal Antibody, Unconjugated (SL1303R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SFRP1) Polyclonal Antibody, Unconjugated (SL1303R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SFRP1) Polyclonal Antibody, Unconjugated (SL1303R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-SFRP1 Polyclonal Antibody, Unconjugated(SL1303R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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