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Rabbit Anti-DLST antibody
Rabbit Anti-DLST antibody
2-oxoglutarate dehydrogenase complex component E2; Dihydrolipoamide S succinyltransferase (E2 component of 2 oxo glutarate complex); Dihydrolipoamide S succinyltransferase; Dihydrolipoamide succinyltransferase component of 2 oxoglutarate dehydrogenase com
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  • NO.:SL13008R
    Clonality:Polyclonal
    Immunogen Species:Rabbit
    React Species:Mouse,(predicted: Human,Rat,Dog,Pig,Cow,Horse,Rabbit,Sheep,)
    Applications:WB ELISA IHC-P IHC-F ICC IF
    concentration:1mg/ml
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Details

Product Name DLST
Chinese Name DLST蛋白抗体
Alias 2-oxoglutarate dehydrogenase complex component E2; Dihydrolipoamide S succinyltransferase (E2 component of 2 oxo glutarate complex); Dihydrolipoamide S succinyltransferase; Dihydrolipoamide succinyltransferase component of 2 oxoglutarate dehydrogenase complex; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex; Dihydrolipoyllysine residue succinyltransferase component of 2 oxoglutarate dehydrogenase complex; Dihydrolipoyllysine residue succinyltransferase component of 2 oxoglutarate dehydrogenase complex mitochondrial; Dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex; Dlst; DLTS; E2; E2K; mitochondrial; ODO2_HUMAN; OGDC-E2.  
Research Area Tumour  Cell biology  lymphocyte  The new supersedes the old  
Immunogen Species Rabbit
Clonality Polyclonal
React Species Mouse,  (predicted: Human, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, )
Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight 41kDa
Cellular localization cytoplasmic 
Form Liquid
Concentration 1mg/ml
immunogen KLH conjugated synthetic peptide derived from human DLST: 201-300/453 
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO2. The complex contains multiple copies of three enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3). DLST (dihydrolipoyllysine-residue succinyltransferase component of 2-oxoglutarate dehydrogenase complex, mitochondrial), also known as DLTS or 2-oxoglutarate dehydrogenase complex component E2, is a 453 amino acid protein belonging to the 2-oxoacid dehydrogenase family. DLST covalently binds one lipoyl cofactor and participates in L-lysine degradation via the saccharopine pathway. Localized to the mitochondrion, DLST forms a 24-polypeptide structural core with octahedral symmetry. The gene encoding DLST maps to human chromosome 14q24.3 and mouse chromosome 12 D2.

Function:
The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).

Subunit:
Forms a 24-polypeptide structural core with octahedral symmetry.

Subcellular Location:
Mitochondrion.

Similarity:
Belongs to the 2-oxoacid dehydrogenase family.
Contains 1 lipoyl-binding domain.

SWISS:
P36957

Gene ID:
1743

Database links:

Entrez Gene: 1743 Human

Entrez Gene: 78920 Mouse

Entrez Gene: 299201 Rat

Omim: 126063 Human

SwissProt: P36957 Human

SwissProt: Q9D2G2 Mouse

SwissProt: Q01205 Rat

Unigene: 525459 Human

Unigene: 296221 Mouse

Unigene: 99702 Rat



Product Picture
Sample: Liver (Mouse) Lysate at 40 ug
Primary: Anti-DLST (SL13008R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 41 kD
Observed band size: 47 kD

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