Product Name	CD28
Chinese Name	CD28抗体
Alias	CD 28; CD28 antigen; CD28 molecule; MGC138290; T cell antigen CD28; T cell specific surface glycoprotein; T cell specific surface glycoprotein CD28; Tp44; CD28_HUMAN; CD28_MOUSE.
literatures	Specific References  (1)     |     SL1297R has been referenced in 1 publications. [IF=2.06] Liu, Shangjing, et al. ʺCostimulatory Molecule CD28 Participates in the Process of Embryo Implantation in Mice.ʺ Reproductive Sciences (2013): 1933719113512537.  IHC-F ;  Mouse.   PubMed:24336670
Research Area	Cell biology  immunology  Stem cells  Cell adhesion molecule  Cell Surface Molecule  lymphocyte  t-lymphocyte  b-lymphocyte
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Chicken, Dog, Pig, Cow, Horse, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	25kDa
Detection molecular weight	45kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from mouse CD28: 1-120/218 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011] Function: Possibly involved in T-cell activation. Subunit: Homodimer; disulfide-linked. Interacts with DUSP14. Binds to CD80/B7-1 and CD86/B7-2/B70. Subcellular Location: Membrane; Single-pass type I membrane protein. Similarity: Contains 1 Ig-like V-type (immunoglobulin-like) domain. SWISS: P31041 Gene ID: 12487 Database links: Entrez Gene: 940 Human Entrez Gene: 12487 Mouse Omim: 186760 Human SwissProt: P10747 Human SwissProt: P31041 Mouse SwissProt: P42069 Rabbit Unigene: 591629 Human Unigene: 255003 Mouse CD28是免疫球蛋白超家族成员之一 ，为T细胞特异性由二硫键连接的同源二聚体表面glycoprotein，主要分布在外周血lymphocyte。对迟发型超敏反应以及Tc介导的细胞毒作用都有免疫调节作用。
Product Picture	Paraformaldehyde-fixed, paraffin embedded (rat stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CD28) Polyclonal Antibody, Unconjugated (SL1297R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: mouse lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Incubation: Anti-CD28 Polyclonal Antibody, Unconjugated(SL1297R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat colitis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37°C for 20 min; Incubation: Anti-CD28 Polyclonal Antibody, Unconjugated(SL1297R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Positive control: (mo)Splenocytes(2% Paraformaldehyde-fixed ) Isotype Control Antibody Antibody: Rabbit IgG; Supplier: Cell Signaling Technology; Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA Secondary Antibody Antibody: Goat anti-rabbit IgG-FITC; Supplier: Cell Signaling Technology; Dilution: 1:200 in 1 X PBS containing 0.5% BSA Primary Antibody Supplier: Bioss; Supplier catalog number: SL1297R; Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA

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