Product Name	NR1H4
Chinese Name	胆汁酸受体抗体
Alias	Bile Acid Receptor NR1H4; BAR; FXR; Farnesoid X activated receptor; Farnesoid X receptor; Farnesoid X-activated receptor; Farnesol receptor HRR 1; Farnesol receptor HRR-1; Farnesol receptor HRR1; FXR; HRR 1; HRR1;NR1H4_HUMAN; Nuclear receptor subfamily 1 group H member 4; Retinoid X receptor interacting protein 14; Retinoid X receptor-interacting protein 14; RIP 14; RIP14; RXR interacting protein 14; RXR-interacting protein 14.
literatures	Specific References  (22)     |     SL12867R has been referenced in 22 publications. [IF=8.4] Zhang, Qiankun, et al. "Effects of the fibrous topography-mediated macrophage phenotype transition on the recruitment of mesenchymal stem cells: An in vivo study." Biomaterials (2017)  WB ;  Mouse.   PubMed:29017079 [IF=7] Zhang, Yaxin. et al. Stigmasterol attenuates hepatic steatosis in rats by strengthening the intestinal barrier and improving bile acid metabolism. npj Science of Food. 2022 Aug;6(1):1-14  WB, IF ;  Rat.   PubMed:10.1038/s41538-022-00156-0 [IF=6.78] Jie Wang. et al. Obeticholic acid aggravates liver injury by up-regulating the liver expression of osteopontin in obstructive cholestasis. LIFE SCI. 2022 Oct;307:120882  WB ;  Mouse.   PubMed:35963300 [IF=6.168] Kong, Weichao. et al. iNKT17 cells play a pathogenic role in ethinylestradiol-induced cholestatic hepatotoxicity. ARCH TOXICOL. 2022 Nov;:1-20  IHC ;  Mouse.   PubMed:36329302 [IF=6.073] Fan Yadong. et al. Abnormal bile acid metabolism is an important feature of gut microbiota and fecal metabolites in patients with slow transit constipation. FRONT CELL INFECT MI. 2022 Jul;0:1060  IHC, WB ;  Human.   PubMed:35967856 [IF=5.717] Jianlong Du. et al. FXR, a Key Regulator of Lipid Metabolism, Is Inhibited by ER Stress-Mediated Activation of JNK and p38 MAPK in Large Yellow Croakers (Larimichthys crocea) Fed High Fat Diets. Nutrients. 2021 Dec;13(12):4343  WB ;  Fish.   PubMed:34959897 [IF=4.679] Mengzhi Zou. et al. The role of invariant natural killer T cells and associated immunoregulatory factors in triptolide-induced cholestatic liver injury. Food Chem Toxicol. 2020 Dec;146:111777  WB ;  Mouse.   PubMed:32987112 [IF=4.545] Yong-li Hua. et al. Baitouweng Tang ameliorates DSS-induced ulcerative colitis through the regulation of the gut microbiota and bile acids via pathways involving FXR and TGR5. Biomed Pharmacother. 2021 May;137:111320  WB ;  Mouse.   PubMed:33578232 [IF=4.414] Liu Y et al. Fish oil alleviates circadian bile composition dysregulation in male mice with NAFLD. J Nutr Biochem. 2019 Apr 4;69:53-62.  WB ;  Mouse.   PubMed:31055233 [IF=4.4] Yu et al. SRT1720 Alleviates ANIT-Induced Cholestasis in a Mouse Model. (2017) Front.Pharmaco. 8:256  WB ;  Mouse.   PubMed:28553227 [IF=4.221] Li Xu. et al. Yinchenhao Tang alleviates high fat diet induced NAFLD by increasing NR1H4 and APOA1 expression. Journal of Traditional and Complementary Medicine. 2023 Feb;:  IHC,WB ;  Mouse.   PubMed:10.1016/j.jtcme.2023.02.010 [IF=4.18] Li T et al. Picroside II protects against cholestatic liver injury possibly through activation of farnesoid X receptor. Phytomedicine,2019. 153153.  WB ;  Mouse.   PubMed:doi:10.1016/j.phymed.2019.153153 [IF=3.85] Guo, Hong-li, et al. "Pyrazinamide induced rat cholestatic liver injury through inhibition of FXR regulatory effect on bile acid synthesis and transport."Toxicological Sciences (2016): kfw098.  WB ;  Rat.   PubMed:27255380 [IF=3.85] Li, Xiaojiaoyang, et al. "UDCA and CDCA alleviate 17α-ethinylestradiol-induced cholestasis through PKA-AMPK pathways in rats." Toxicology and Applied Pharmacology 311 (2016): 12-25.  WB ;  Human.   PubMed:27743861 [IF=3.82] Zhao, Guolin, et al. "8-Methoxypsoralen disrupts MDR3-mediated phospholipids efflux and bile acid homeostasis and its relevance to hepatotoxicity." Toxicology (2017).  WB ;  Rat.   PubMed:28552422 [IF=3.77] Yang, Tingting, et al. "Early indications of ANIT-induced cholestatic liver injury: Alteration of hepatocyte polarization and bile acid homeostasis." Food and Chemical Toxicology (2017).  IHC-P ;  Rat.   PubMed:28986171 [IF=3.71] Zhou et al. Retinoic acid induces macrophage cholesterol efflux and inhibits atherosclerotic plaque formation in apoE-deficient mice. (2015) Br.J.Nutr. 114:509-18  WB ;  Mouse.   PubMed:26201974 [IF=3.628] Peishi Liang. et al. Obeticholic acid improved triptolide/lipopolysaccharide-induced hepatotoxicity by inhibiting Caspase-11-GSDMD pyroptosis pathway. J APPL TOXICOL. 2022 Nov;:  WB ;  Mouse.   PubMed:36328986 [IF=3.616] Zhao G et al. Adaptive homeostasis of the vitamin D–vitamin D nuclear receptor axis in 8-methoxypsoralen-induced hepatotoxicity. (2018) Toxicology and Applied Pharmacology. Jan 1;362:150-158.  WB ;  Rat.   PubMed:30419252 [IF=3.547] Yuan Z et al. A new perspective of triptolide-associated hepatotoxicity: Liver hypersensitivity upon LPS stimulation. Toxicology. 2019 Feb 15;414:45-56.  WB ;  Mouse.   PubMed:30633930 [IF=3.52] Yu, Linxi, et al. "Protective effects of SRT1720 via the HNF1α/FXR signalling pathway and anti-inflammatory mechanisms in mice with estrogen-induced cholestatic liver injury." Toxicology Letters (2016).  WB ;  Mouse.   PubMed:27818225 [IF=2.629] Chen Lisheng. et al. Paeoniflorin Protects against ANIT-Induced Cholestatic Liver Injury in Rats via the Activation of SIRT1-FXR Signaling Pathway. Evid-Based Compl Alt. 2021;2021:8479868  WB ;  rat.   PubMed:34512782
Research Area	Tumour  Cell biology  Signal transduction  The new supersedes the old  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Dog, Pig, Cow, Horse, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=3ug/test ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	56kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human FXR/Bile Acid Receptor NR1H4: 175-280/486
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The steroid receptor superfamily acts through direct association with DNA sequences known as hormone response elements (HREs) and binds DNA as either homo- or heterodimers. The promiscuous mediator of heterodimerization, RXR, is the receptor for 9-cis retinoic acid, and dimerizes with VDR, TR, PPAR, and several novel receptors including LXR (also referred to as RLD-1) and FXR. FXR and LXR fall into a category of proteins termed “orphan receptors” because of their lack of a defined function, and in the case of LXR, the lack of a defined ligand. FXR has been shown to bind a class of lipid molecules called farnesoids. LXR/RXR heterodimers have highest affinity for DR-4 DNA elements while FXR/RXR heterodimers bind IR-1 elements. Both LXR/RXR and FXR/RXR heterodimers retain their responsiveness to 9-cis retinoic acid. Function: Ligand-activated transcription factor. Receptor for bile acids such as chenodeoxycholic acid, lithocholic acid and deoxycholic acid. Represses the transcription of the cholesterol 7-alpha-hydroxylase gene (CYP7A1) through the induction of NR0B2 or FGF19 expression, via two distinct mechanisms. Activates the intestinal bile acid-binding protein (IBABP). Activates the transcription of bile salt export pump ABCB11 by directly recruiting histone methyltransferase CARM1 to this locus. Subunit: eterodimer of NR1H4 and RXR. After activation by agonist binding, interacts with a coactivator, NCOA1 or NCOA2 (By similarity). Interacts with CARM1 and SMARD1. Subcellular Location: Nucleus. Post-translational modifications: Methylation may increase transactivation of target genes. Similarity: Belongs to the nuclear hormone receptor family. NR1 subfamily. Contains 1 nuclear receptor DNA-binding domain. SWISS: Q96RI1 Gene ID: 9971 Database links: Entrez Gene: 9971 Human Entrez Gene: 20186 Mouse Entrez Gene: 60351 Rat Omim: 603826 Human SwissProt: Q96RI1 Human SwissProt: Q3V1T8 Mouse SwissProt: Q60641 Mouse SwissProt: Q5XI75 Rat SwissProt: Q62735 Rat Unigene: 282735 Human Unigene: 3095 Mouse Unigene: 42943 Rat
Product Picture	Sample: Large intestine (Mouse) Lysate at 40 ug Primary: Anti-NR1H4 (SL12867R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kD Sample: Lane 1: Mouse Kidney tissue lysates Lane 2: Mouse Pancreas tissue lysates Lane 3: Rat Liver tissue lysates Lane 4: Human HepG2 cell lysates Lane 5: Human MCF-7 cell lysates Primary: Anti- NR1H4 (SL12867R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kDa Observed band size: 50 kDa Sample: HepG2 (Human) CellLysate at 30 ug Primary: Anti-NR1H4 (SL12867R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kD Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti- NR1H4 (SL12867R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kD Sample: HepG2 Cell (Human) Lysate at 40 ug Primary: Anti-NR1H4 (SL12867R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56 kD Observed band size: 56 kD Blank control:A549. Primary Antibody (green line): Rabbit Anti-NR1H4 antibody (SL12867R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 3μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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