Product Name	Phospho1
Chinese Name	细胞质磷酸酶1抗体
Alias	EC 3.1.3.75; PHOP1_HUMAN; Phosphatase orphan 1; Phosphatase, orphan 1; Phospho 1; Phosphoethanolamine/phosphocholine phosphatase.
Research Area	Cell biology  Developmental biology  Signal transduction  Stem cells  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Dog, Pig, Cow, Horse, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	30kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Phospho1: 201-267/267
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	PHOSPHO1 is a 267 amino acid phosphatase that is a member of the haloacid dehalogenase (HAD) superfamily of magnesium-dependent hydrolases. PHOSPHO1 is highly expressed in bone and cartilage and localizes to the osteoid layer of the periosteum. PHOSPHO1 is restricted to sites of mineralization and its inhibition decreases the ability of matrix vesicles to calcify in bone, suggesting that the protein may play a role in the matrix mineralization process during skeletal development. PHOSPHO1 cleaves phosphoethanolamine and phosphocholine to generate inorganic phosphate for bone mineralization. PHOSPHO1 contains three catalytic motifs that are conserved within the haloacid dehalogenase superfamily. Function: Phosphatase that has a high activity toward phosphoethanolamine (PEA) and phosphocholine (PCho). Involved in the generation of inorganic phosphate for bone mineralization. Tissue Specificity: Expressed at sites of mineralization in bone and cartilage. Highly expressed in osteoblast cell line SaOS-2 which produces a mineralized matrix, but not in MG-63 cell line, which do not mineralize. Similarity: Belongs to the HAD-like hydrolase superfamily. PHOSPHO family. SWISS: Q8TCT1 Gene ID: 162466 Database links: Entrez Gene: 162466 Human Entrez Gene: 237928 Mouse Entrez Gene: 287644 Rat SwissProt: Q8TCT1 Human SwissProt: Q8R2H9 Mouse SwissProt: B5DFB9 Rat
Product Picture	Sample: Hela Cell (Human) Lysate at 40 ug Primary: Anti-Phospho1 (SL12562R) at 1/300 dilution Secondary: HRP conjugated Goat-Anti-rabbit IgG (SL0295G-HRP) at 1/5000 dilution Predicted band size: 30 kD Observed band size: 30 kD Sample: U87MG(Human) Cell Lysate at 30 ug Primary: Anti- Phospho1 (SL12562R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 30 kD Observed band size: 35 kD Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho1) Polyclonal Antibody, Unconjugated (SL12562R ) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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