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Product Name AQP3 Chinese Name 水Channel protein-3抗体 Alias AQP 3; AQP3; Aquaporin 3 (GIL blood group); Aquaporin 3 (Gill blood group); Aquaporin3; Aquaporin-3; GIL; Gill blood group; AQP3_MOUSE. literatures Specific References (8) | SL1253R has been referenced in 8 publications.Research Area Tumour Signal transduction Channel protein Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, ) Applications ELISA=1:5000-10000 IHC-F=1:100-500 Flow-Cyt=2ug/Test IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 32kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from mouse AQP3: 201-292/292 <Cytoplasmic> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes the water channel protein aquaporin 3. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein, also known as aquaporin 0. Aquaporin 3 is localized at the basal lateral membranes of collecting duct cells in the kidney. In addition to its water channel function, aquaporin 3 has been found to facilitate the transport of nonionic small solutes such as urea and glycerol, but to a smaller degree. It has been suggested that water channels can be functionally heterogeneous and possess water and solute permeation mechanisms. [provided by RefSeq, Aug 2011]
Function:
Water channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism.
Subcellular Location:
Membrane; Multi-pass membrane protein.
Tissue Specificity:
Renal medulla and colon. Predominantly in the inner medulla. Expressed in basal layer of epidermal keratinocytes.
Similarity:
Belongs to the MIP/aquaporin (TC 1.A.8) family.
SWISS:
Q8R2N1
Gene ID:
11828
Database links:Entrez Gene: 360 Human
Entrez Gene: 11828 Mouse
Omim: 600170 Human
SwissProt: Q92482 Human
SwissProt: Q8R2N1 Mouse
Unigene: 234642 Human
Unigene: 34043 Mouse
Unigene: 11109 Rat
Channel protein(Channel Protein)
AQP 3主要分布于肾脏集合管epithelial cells基侧质膜,除了对水有通透性外、还可转运尿素和甘油等小分子物质,而尿素在尿液浓缩机制中有着重要的作用.
有学者认为:AQP-3的阳性率与某些Tumour分化程度有关(胃癌、结肠癌、乳腺癌、前列腺癌、子宫癌、卵巢癌、甲状腺癌等)Product Picture Blank control: Hela.
Primary Antibody (green line): Rabbit Anti-AQP3 antibody (SL1253R)
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.Blank control(black line):A431.
Primary Antibody (green line): Rabbit Anti-AQP3 antibody (SL1253R)
Dilution:1ug/Test;
Secondary Antibody(white blue line): Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Isotype control(orange line): Normal Rabbit IgG
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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