Product Name	ARPC1A
Chinese Name	肌动蛋白相关蛋白2/3亚型1A抗体
Alias	Actin binding protein (Schizosaccharomyces pombe sop2 like); Actin related protein 2/3 complex subunit 1A 41kDa; Actin-related protein 2/3 complex subunit 1A; ARC1A_HUMAN; Arc40; ARPC1A; SOP2-like protein; SOP2Hs; SOP2L.
Research Area	Cell biology  Signal transduction  Cytoskeleton
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Chicken, Pig, Cow, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	42kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human ARPC1A: 101-200/370
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes one of seven subunits of the human Arp2/3 protein complex. This subunit is a member of the SOP2 family of proteins and is most similar to the protein encoded by gene ARPC1B. The similarity between these two proteins suggests that they both may function as p41 subunit of the human Arp2/3 complex that has been implicated in the control of actin polymerization in cells. It is possible that the p41 subunit is involved in assembling and maintaining the structure of the Arp2/3 complex. Multiple versions of the p41 subunit may adapt the functions of the complex to different cell types or developmental stages. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2010]. Function: Probably functions as component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Subunit: Probable component of the Arp2/3 complex in which it may replace ARPC1B. Subcellular Location: Cytoplasm; cytoskeleton. Similarity: Belongs to the WD repeat ARPC1 family. Contains 6 WD repeats. SWISS: Q92747 Gene ID: 10552 Database links: Entrez Gene: 770269 Chicken Entrez Gene: 508402 Cow Entrez Gene: 10552 Human Entrez Gene: 56443 Mouse Entrez Gene: 100174963 Pig Entrez Gene: 81824 Rat Entrez Gene: 379845 Xenopus laevis Entrez Gene: 394565 Xenopus tropicalis Entrez Gene: 394243 Zebrafish Omim: 604220 Human SwissProt: Q1JP79 Cow SwissProt: Q92747 Human SwissProt: Q9R0Q6 Mouse SwissProt: Q99PD4 Rat Unigene: 124126 Human Unigene: 371610 Mouse Unigene: 844 Rat
Product Picture	Sample: testes (Mouse) Lysate at 40 ug Primary: Anti-ARPC1A (SL12526R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 42 kD Tissue/cell: Human cervical cancer; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ARPC1A Polyclonal Antibody, Unconjugated(SL12526R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: Rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ARPC1A Polyclonal Antibody, Unconjugated(SL12526R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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