Product Name	APG8A
Chinese Name	自噬相关蛋白8A抗体
Alias	APG8A (autophagy 8A); AT4G21980; ATG8; ATG8A; Autophagy 8A; Autophagy related protein 8a; Autophagy related ubiquitin like modifier ATG8a; F1N20.80; F1N20_80; ATG8A_ARATH.
Research Area	Cell biology  Signal transduction  Transporter  Epigenetics  Ubiquitin
Immunogen Species	Rabbit
Clonality	Polyclonal
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	13kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from Arabidopsis thaliana APG8A: 1-100/122
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	APG8A is involved in cytoplasm to vacuole transport (Cvt) vesicles and autophagosomes formation. It may mediate the delivery of the vesicles and autophagosomes to the vacuole via the microtubule cytoskeleton. Function: Involved in cytoplasm to vacuole transport (Cvt) vesicles and autophagosomes formation. May mediate the delivery of the vesicles and autophagosomes to the vacuole via the microtubule cytoskeleton. Subunit: Interacts with ATG4b. Subcellular Location: Cytoplasmic vesicle ?cvt vesicle membrane; Lipid-anchor. Cytoplasmic vesicle ?autophagosome membrane; Lipid-anchor. Vacuole membrane; Lipid-anchor. Note= Membrane-associated through a lipid anchor. Post-translational modifications: The C-terminal 5 residues are removed by ATG4 to expose Gly-117 at the C-terminus. This Gly-117 forms then a thioester bond with the 'Cys-558' of ATG7 (E1-like activating enzyme) before being transferred to the 'Cys-258' of ATG3 (the specific E2 conjugating enzyme), in order to be finally amidated with phosphatidylethanolamine. This lipid modification anchors ATG8 to autophagosomes (By similarity). Similarity: Belongs to the MAP1 LC3 family. SWISS: Q8LEM4 Gene ID: 828287 Database links: UniProtKB/Swiss-Prot: Q8LEM4.2

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