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Product Name MAPK6 Chinese Name 丝裂原活化蛋白激酶6抗体 Alias ERK-3; ERK3; ERK 3; Extracellular signal regulated kinase 3; Extracellular signal regulated kinase p97; Extracellular signal-regulated kinase 3; MAP kinase 6; MAP kinase isoform p97; MAPK 6;Mitogen activated protein kinase 6; Mitogen-activated protein kinase 6; MK06_HUMAN; p97 MAPK; p97-MAPK; PRKM6; Protein kinase mitogen activated 5; Protein kinase mitogen activated 6. Research Area Cell biology Signal transduction Stem cells Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 83kDa Cellular localization The nucleus cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human MAPK6/ERK3: 21-120/721 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Mitogen-activated protein kinase (MAPK) signaling pathways involve closely related MAP kinases, including extracellular-signal-related kinase 3 (ERK 3, also designated PRKM6 and p97MAPK). Serum, growth factors and phorbol esters can initiate ERK 3 signaling pathways. Despite lacking a definitive nuclear localization sequence, ERK 3 constitutively localizes to the nucleus upon activation. p38 pathway activation-dependent upregulation of ERK 3 is independent of the status of p53, Bcl-2 and caspase-3 during cell stress and damage induced by proteasome inhibition, suggesting ERK 3 in part mediates intracellular defense or cell rescue. The human ERK 3 gene maps to chromosome 15q21.2 and encodes a 721 amino acid protein.
Function:
Phosphorylates microtubule-associated protein 2 (MAP2). May promote entry in the cell cycle.
Subunit:
Heterodimer with ERK4/MAPK4. Interacts with (via FRIEDE motif) MAPKAPK5 (By similarity). Interacts with UBE3A; this interaction may be indirect and mediated by HERC2, possibly via HERC2 interaction with NEURL4.
Subcellular Location:
Cytoplasm (By similarity). Nucleus (By similarity). Note=Translocates to the cytoplasm following interaction with MAPKAPK5 (By similarity).
Tissue Specificity:
Highest expression in the skeletal muscle, followed by the brain. Also found in heart, placenta, lung, liver, pancreas, kidney and skin fibroblasts.
Post-translational modifications:
Dually phosphorylated on Thr-626 and Tyr-628, which activates the enzyme.
Similarity:
Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family.
MAP kinase subfamily.
Contains 1 protein kinase domain.
SWISS:
Q16659
Gene ID:
5597
Database links:Entrez Gene: 5597 Human
Entrez Gene: 50772 Mouse
Omim: 602904 Human
SwissProt: Q16659 Human
SwissProt: Q61532 Mouse
Unigene: 411847 Human
Unigene: 480076 Mouse
Unigene: 88457 Rat
Product Picture Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MAPK6) Polyclonal Antibody, Unconjugated (SL12405R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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