Product Name	OSR1 + OSR2
Chinese Name	OSR1/OSR2蛋白抗体
Alias	FLJ90037; Odd skipped related 2; Odd skipped related 2A protein; Odd-skipped related; odd-skipped related 2 (Drosophila); Osr2; OSR2_HUMAN; Protein odd-skipped-related 2; ODD; Odd skipped Drosophila homolog; Odd skipped homolog; Odd skipped related 1 (Drosophila); Odd skipped related 1; Odd skipped related 1 Drosophila; Odd1; Protein odd skipped related 1; OSR1_HUMAN.
Research Area	Cell biology  Developmental biology  Stem cells  Zinc finger protein  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Dog, Pig, Cow, Horse, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	35kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human OSR2 and OSR1: 181-280/312
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	OSR2 is a 312 amino acid protein that contains five zinc finger domains. It is expressed in the kidneys, skeletal muscle, testis, and mouse embryos and may be involved in transcriptional activity and osteoblast function. The expression of OSR2 is regulated by C/EBP regulatory elements. OSR2 plays a role in regulating palatal development and expression of alkaline phosphatase. Two isoforms, OSR2A and OSR2B, are produced due to alternative splicing. OSR2B is 36 amino acids shorter than OSR2A and contains only three zinc finger motifs. Both isoforms localize to the nucleus and are thought to exhibit opposite transcriptional activities. Mutations in the gene encoding OSR2 can alter the gene expression of Pax-9 and TGF beta3. Subcellular Location: Nucleus. Similarity: Belongs to the Odd C2H2-type zinc-finger protein family. Contains 5 C2H2-type zinc fingers. SWISS: Q8TAX0 Q8N2R0 Gene ID: 130497 116039 Database links: Entrez Gene: 539430 Cow Entrez Gene: 130497 Human Entrez Gene: 23967 Mouse Entrez Gene: 298878 Rat Omim: 608891 Human SwissProt: Q08DS3 Cow SwissProt: Q8TAX0 Human SwissProt: Q9WVG7 Mouse SwissProt: B0K011 Rat Entrez Gene: 116039 Human Entrez Gene: 107587 Mouse Entrez Gene: 315039 Rat Omim: 611297 Human SwissProt: Q8N2R0 Human SwissProt: Q91ZD1 Mouse SwissProt: Q6AY34 Rat Unigene: 253247 Human Unigene: 46336 Mouse Unigene: 13228 Rat
Product Picture	Tissue/cell: mouse embryo tissue ; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-OSR2 Polyclonal Antibody, Unconjugated(SL12377R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OSR1 + OSR2) Polyclonal Antibody, Unconjugated (SL12377R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.

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