TEL: +86 571 56623320 EMAIL: [email protected]
Product Name AATF Chinese Name 拮抗凋亡转录因子抗体 Alias Apoptosis antagonizing transcription factor; CHE1; DED; AATF_HUMAN; Apoptosis-antagonizing transcription factor; Rb-binding protein Che-1; Protein AATF;Rb binding protein Che 1. Research Area Tumour Cell biology immunology Chromatin and nuclear signals Apoptosis transcriptional regulatory factor Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Pig, Cow, Horse, Rabbit, Guinea Pig, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 63kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human AATF: 451-560/560 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The protein encoded by this gene was identified on the basis of its interaction with MAP3K12/DLK, a protein kinase known to be involved in the induction of cell apoptosis. This gene product contains a leucine zipper, which is a characteristic motif of transcription factors, and was shown to exhibit strong transactivation activity when fused to Gal4 DNA binding domain. Overexpression of this gene interfered with MAP3K12 induced apoptosis. [provided by RefSeq, Jul 2008]
Function:
May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
Subunit:
Binds PAWR, POLR2J, RB1/RB, RBL1/P107, RBL2/P130, and SP1. May also bind MAPT.
Subcellular Location:
Nucleus, nucleolus.
Tissue Specificity:
Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.
Post-translational modifications:
Hyperphosphorylated during the G1/S phase transition.
Similarity:
Belongs to the AATF family.
SWISS:
Q9NY61
Gene ID:
26574
Database links:Entrez Gene: 26574 Human
Entrez Gene: 56321 Mouse
Omim: 608463 Human
SwissProt: Q9NY61 Human
SwissProt: Q3URN5 Mouse
SwissProt: Q9JKX4 Mouse
Unigene: 195740 Human
Unigene: 257482 Mouse
AATF属于Apoptosis的拮抗转录因子,有抑制Apoptosis的发展过程。Product Picture Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-AATF Polyclonal Antibody, Unconjugated(SL1229R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Scan Wechat Qrcode