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Product Name ZNF740 Chinese Name Zinc finger protein740抗体 Alias MGC61706; OriLyt TD-element-binding protein 7; TB7; Zfp740; Zinc finger protein 740; ZN740_HUMAN; ZNF740. Research Area transcriptional regulatory factor Zinc finger protein Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, (predicted: Cow, Rabbit, Sheep, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 22kDa Cellular localization The nucleus Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from Human ZNF740: 101-193/193 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail May be involved in transcriptional regulation.
Function:
May be involved in transcriptional regulation.
Subcellular Location:
Nucleus.
Similarity:
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 3 C2H2-type zinc fingers.
SWISS:
Q8NDX6
Gene ID:
283337
Database links:Entrez Gene: 283337 Human
SwissProt: Q8NDX6 Human
Unigene: 524458 Human
Product Picture Sample: Kidney (Mouse) Lysate at 40 ug
Primary: Anti-ZNF740 (SL12227R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 22 kD
Observed band size: 22 kD
Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ZNF740 Polyclonal Antibody, Unconjugated(SL12227R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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