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Product Name DZIP3 Chinese Name RNA结合Ubiquitin连接酶DZIP3抗体 Alias DAZ-interacting protein 3; DAZ interacting protein 3, zinc finger; E3 ubiquitin-protein ligase DZIP3; hRUL138; KIAA0675; UURF2; RNA-binding ubiquitin ligase of 138 kDa; DZIP3_HUMAN. Research Area Cardiovascular Cell biology Zinc finger protein Epigenetics Ubiquitin Immunogen Species Rabbit Clonality Polyclonal React Species Mouse, (predicted: Human, Rat, Dog, Cow, Horse, Rabbit, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 139kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from Human DZIP3: 245-350/1208 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail DZIP3 is a 1,208 amino acid protein that localizes to the cytoplasm and contains one RING-type zinc finger. Expressed in a variety of tissues with highest expression in heart, skeletal muscle and kidney, DZIP3 functions as an E3 ubiquitin-protein ligase that accepts ubiquitin from an E2 ubiquitin-conjugating enzyme, thereby playing a role in signaling events throughout the cell. Multiple isoforms of DZIP3 exist due to alternative splicing events.
Function:
E3 Ubiquitin ligase proteins mediate ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Able to specifically bind RNA.
Subunit:
Interacts with DAZ proteins.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Widely expressed at low level. Highly expressed in skeletal muscle, kidney and heart. Expressed at low level in placenta, lung, brain, liver and pancreas.
Similarity:
Contains 1 RING-type zinc finger.
SWISS:
Q86Y13
Gene ID:
9666
Database links:Entrez Gene: 9666 Human
SwissProt: Q86Y13 Human
Product Picture Blank control:Mouse kidney.
Primary Antibody (green line): Rabbit Anti-DZIP3 antibody (SL12191R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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