Product Name	ST18
Chinese Name	Tumour抑制蛋白18抗体
Alias	KIAA0535; St18; ST18_HUMAN; suppression of tumorigenicity 18 (breast carcinoma) (zinc finger protein); Suppression of tumorigenicity 18 protein; Suppression of tumorigenicity protein 18; Zinc finger protein 387; Znf387.
Research Area	Tumour  Cell biology  Stem cells  Zinc finger protein  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Horse, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=3ug/Test ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	115kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human ST18/Znf387: 451-550/1047
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	ST18 is a 1,047 amino acid nuclear protein that is expressed at low levels in heart, liver, kidney, skeletal muscle, pancreas, testis, ovary and prostate. Containing six C2HC-type zinc fingers and an SMC domain, ST18 is a repressor protein that binds to DNA sequences containing a specific bipartite element. ST18 is considered a transcription factor that represses basal transcription activity from target promoters and Inhibits colony formation in cultured breast cancer cells. ST18 is encoded by a gene located on human chromosome 8, which consists of nearly 146 million base pairs, encodes over 800 genes and is associated with a variety of diseases and malignancies. Schizophrenia, bipolar disorder, Trisomy 8, Pfeiffer syndrome, congenital hypothyroidism, Waardenburg syndrome and some leukemias and lymphomas are thought to occur as a result of defects in specific genes that map to chromosome 8. Function: Repressor that binds to DNA sequences containing a bipartite element consisting of a direct repeat of the sequence 5'-AAAGTTT-3' separated by 2-9 nucleotides. Represses basal transcription activity from target promoters (By similarity). Inhibits colony formation in cultured breast cancer cells. Subcellular Location: Nucleus. Tissue Specificity: Detected at low levels in heart, liver, kidney, skeletal muscle, pancreas, testis, ovary and prostate. Detected at even lower levels in mammary epithelial cells and breast cancer cells. Similarity: Contains 6 C2HC-type zinc fingers. SWISS: O60284 Gene ID: 9705 Database links: Entrez Gene: 9705 Human Entrez Gene: 240690 Mouse Entrez Gene: 266680 Rat SwissProt: O60284 Human SwissProt: Q80TY4 Mouse SwissProt: Q9QX27 Rat Unigene: 655499 Human Unigene: 234612 Mouse Unigene: 91311 Rat
Product Picture	Sample: Cerebrum (Mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug Primary: Anti- ST18 (SL12188R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115 kD Observed band size: 110 kD Sample: U87MG(Human) Cell Lysate at 30 ug Primary: Anti- ST18 (SL12188R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 115 kD Observed band size: 115 kD Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ST18 Polyclonal Antibody, Unconjugated(SL12188R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: Mouse kidney. Primary Antibody (green line): Rabbit Anti-ST18 antibody (SL12188R) Dilution: 3μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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