Product Name	P2Y8
Chinese Name	G蛋白偶联嘌呤受体p2y8抗体
Alias	P2RY8; P2RY8_HUMAN; P2Y purinoceptor 8; P2Y8; Purinergic receptor P2Y G protein coupled 8.
Research Area	Neurobiology  Channel protein  The cell membrane受体  G protein-coupled receptor  G protein signal
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	41kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human P2Y8: 131-230/359 <Extracellular>
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	G protein-coupled receptors (GPRs), also known as seven transmembrane receptors, heptahelical receptors or 7TM receptors, comprise a superfamily of proteins that play a role in many different stimulus-response pathways. G-protein coupled receptors translate extracellular signals into intracellular signals (G-protein activation) and they respond to a variety of signaling molecules, such as hormones and neurotransmitters. P2RY8 (purinergic receptor P2Y, G-protein coupled, 8), also known as P2Y8, is a 359 amino acid multi-pass membrane protein that localizes to the cell membrane and belongs to the G protein-coupled receptor family. Expressed at low levels in lung, heart and kidney, P2RY8 may function as a receptor for purines that are coupled to G proteins and may also play a role in mental retardation. Function: Probable receptor for purines coupled to G-proteins. Subcellular Location: Cell membrane; Multi-pass membrane protein. Tissue Specificity: Barely detectable in normal blood leukocytes. Weaker expression was seen in heart, kidney and lung. Not detected in brain. Similarity: Belongs to the G-protein coupled receptor 1 family. SWISS: Q86VZ1 Gene ID: 286530 Database links: Entrez Gene: 286530 Human Omim: 300525 Human SwissProt: Q86VZ1 Human Unigene: 111377 Human
Product Picture	Sample: Raji Cell (Human) Lysate at 30 ug Primary: Anti- P2Y8 (SL12073R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 66 kD Observed band size: 50 kD Blank control: HL60. Primary Antibody (green line): Rabbit Anti-P2Y8 antibody (SL12073R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (blue line): Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control: Raji. Primary Antibody (green line): Rabbit Anti-P2Y8 antibody (SL12073R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF488 Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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