Product Name	ROCK2
Chinese Name	Rho相关蛋白激酶2抗体
Alias	p164 ROCK 2; p164 ROCK-2; Rho associated coiled coil containing protein kinase 2; Rho associated protein kinase 2; Rho kinase 2; ROCK 2; Rock II; Rock2m; ROK alpha; ROKalpha; coiled-coil-containing protein kinase 2; KIAA0619; Rho associated, coiled coil containing protein kinase II; Rho-associated; Rho-associated protein kinase 2; Rock2; ROCK2_HUMAN.
literatures	Specific References  (6)     |     SL1205R has been referenced in 6 publications. [IF=4.26] Zhu et al. Design and evaluation of EphrinA1 mutants with cerebral protective effect. (2017) Sci.Re. 7:1881  WB ;  Human,Mouse.   PubMed:28507312 [IF=4.19] He, Hongpeng, et al. "Transcription of HOTAIR is regulated by RhoC-MRTF-A-SRF signaling pathway in human breast cancer cells." Cellular Signalling (2017).  WB ;  Human.   PubMed:28069441 [IF=4.15] Wang, Nan, et al. "Vascular endothelial growth factor stimulates endothelial differentiation from mesenchymal stem cells via Rho/myocardin-related transcription factor-A signaling pathway." The International Journal of Biochemistry & Cell Biology (2013).  WB ;  Rat.   PubMed:23624342 [IF=4.12] Rothschild et al. ROCK-1 mediates diabetes-induced retinal pigment epithelial and endothelial cell blebbing: Contribution to diabetic retinopathy. (2017) Sci.Rep. 7:8834  IF ;  Rat.   PubMed:28821742 [IF=3.457] Meng C et al. MRTF-A mediates the activation of COL1A1 expression stimulated by multiple signaling pathways in human breast cancer cells.Biomed Pharmacother. 2018 Aug;104:718-728.  WB ;  Human.   PubMed:29807221 [IF=3.231] Gui-Long Zhang. et al. Effect of conditioned medium from neural stem cells on glioma progression and its protein expression profile analysis. World J Stem Cells. 2020 Nov 26; 12(11): 1396–1409  WB ;  Human.   PubMed:33312406
Research Area	Tumour  Cell biology  Signal transduction  Apoptosis  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	161kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human ROCK2: 1001-1300/1388
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho. [provided by RefSeq, Jul 2008]. Function: Regulates the assembly of the actin cytoskeleton. Promotes formation of stress fibers and of focal adhesion complexes. Plays a role in smooth muscle contraction. Subcellular Location: Cytoplasm. Cell membrane. Cytoplasmic, and associated with actin microfilaments and the plasma membrane. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR.Phosphorylation at Tyr-722 reduces its binding to RHOA and iscrucial for focal adhesion dynamics. Dephosphorylation by PTPN11stimulates its RHOA binding activity. Cleaved by granzyme B during apoptosis. This leads toconstitutive activation of the kinase and membrane blebbing. Similarity: Belongs to the protein kinase superfamily. AGC Ser/Thrprotein kinase family. Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. Contains 1 phorbol-ester/DAG-type zinc finger. Contains 1 protein kinase domain. Contains 1 REM (Hr1) repeat. SWISS: O75116 Gene ID: 9475 Database links: Entrez Gene: 9475 Human Entrez Gene: 19878 Mouse Entrez Gene: 25537 Rat Omim: 604002 Human SwissProt: O75116 Human SwissProt: P70336 Mouse ROCK2（Rho kinase alpha）是ROCK1的同工酶。是一个丝/苏氨基酸激酶，可调节cell factor，平滑肌收缩和肌动蛋白的形成。
Product Picture	Sample: Lane 1: Mouse Heart Lysates Lane 2: Mouse Skeletal muscle Lysates Primary: Anti-ROCK2 (SL1205R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 161kDa Observed band size: 180kDa Sample: Lane1: Brain (Rat) Lysate at 30 ug Lane2: Colon carcinoma (Human) Lysate at 30 ug Primary: Anti-ROCK2 (SL1205R) at 1:200 dilution; Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution; Predicted band size : 161kD Observed band size : 161kD Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-ROCK2 (SL1205R) at 1/500 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 161 kD Observed band size: 161 kD Tissue/cell: human gastric carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ROCK2 Polyclonal Antibody, Unconjugated(SL1205R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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