Product Name	PAX8
Chinese Name	配对盒基因8抗体
Alias	Paired Box Gene 8; paired box homeotic gene 8; Paired box protein Pax 8; Paired Domain Gene 8; PAX 8;PAX-8; PAX8_HUMAN.
literatures	Specific References  (5)     |     SL1201R has been referenced in 5 publications. [IF=4.872] Dong X et al. PM2.5 disrupts thyroid hormone homeostasis through activation of the hypothalamic-pituitary-thyroid (HPT) axis and induction of hepatic transthyretin in female rats 2.5Ecotoxicol Environ Saf.2021 Jan 15;208:111720.  IHC、WB ;  Rat.   PubMed:33396051 [IF=4.711] Xiuzhen Du. et al. Membranous and nuclear staining of CLDN18 in HPV-independent and HPV-associated endocervical adenocarcinomas. CANCER MED-US. 2022 Jul;:  IHC ;  Human.   PubMed:35861118 [IF=4.223] Dong, Xinwen. et al. Protective effects of curcumin against thyroid hormone imbalance after gas explosion-induced traumatic brain injury via activation of the hypothalamic-pituitary-thyroid axis in male rats. ENVIRON SCI POLLUT R. 2022 May;:1-13  WB,IHC ;  Rat.   PubMed:35641736 [IF=2.34] Huang et al. Upregulation of TSHR, TTF-1, and PAX8 in Nodular Goiter Is Associated with Iodine Deficiency in the Follicular Lumen. (2016) Int.J.Endocrinol. 2016:2492450  IHC,WB ;  Human.   PubMed:27525008 [IF=1.38] Vadasz, Stephanie, et al. "Second and third trimester amniotic fluid mesenchymal stem cells can repopulate a de-cellularized lung scaffold and express lung markers." Journal of Pediatric Surgery (2014).  Human.   PubMed:25475793
Research Area	Developmental biology  Neurobiology  transcriptional regulatory factor  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Dog, Cow, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	48kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PAX8: 21-130/457
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This protein is a member of the paired box (PAX) family of transcription factors, typically containing a paired box domain, an octapeptide, and a paired-type homeodomain. This family plays critical roles during fetal development and cancer growth. The specific function of the PAX8 is unknown but it may involve kidney cell differentiation, thyroid development, or thyroid dysgenesis. Alternative splicing in the gene by inclusion or exclusion of exons 7 and/or 8 has produced several known products but the biological significance of the variants is unknown. Several other splice variants have been proposed but the full nature of these products has not been described. Pax8 is also a marker of otic progenitor cells. Function: Transcription factor for the thyroid-specific expression of the genes exclusively expressed in the thyroid cell type, maintaining the functional differentiation of such cells. Subunit: Interacts with WWTR1. Subcellular Location: Nucleus. Tissue Specificity: Expressed in the excretory system, thyroid gland and Wilms tumors. DISEASE: Hypothyroidism, congenital, non-goitrous, 2 (CHNG2) [MIM:218700]: A disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Contains 1 paired domain. SWISS: Q06710 Gene ID: 7849 Database links: Entrez Gene: 7849 Human Entrez Gene: 18510 Mouse Entrez Gene: 81819 Rat Omim: 167415 Human SwissProt: Q06710 Human SwissProt: Q00288 Mouse SwissProt: P51974 Rat Unigene: 469728 Human Unigene: 2533 Mouse Unigene: 10392 Rat 同源结构域蛋白（Homeodomain Proteins） PAX8属于转录抑制因子PAXs的一种。在胚胎发育和Tumour生长中起关键作用。其突变和某些Tumour的发病有关。
Product Picture	Tissue/cell: rat colon tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PAX8 Polyclonal Antibody, Unconjugated(SL1201R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining U87MG cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (PAX8) polyclonal Antibody, Unconjugated (SL1201R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Cell:HT-29; Concentration:1:100 Host/Isotype:Rabbit/IgG(blue line) Flow cytometric analysis of Rabbit IgG isotype control on HT-29(blue) compared with primary antibody(Cat#: SL1021R)(green) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG(H+L) secondary antibody . Primary Antibody:Rabbit Anti-TLR4 antibody(SL1021R, green line); Isotype Control Antibody: Rabbit IgG (orange line); Secondary Antibody: F(ab’)2 fragment goat anti-rabbit IgG-PE (white blue line) Positive control: Jurkat cells(blue line) Protocol 1.Jurkat cells were washed twice with phosphate-buffered saline (PBS). 2. An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 1 0 min at 37 °C.

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