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Product Name CHRM4 Chinese Name 毒蕈碱型乙酰胆碱受体M4抗体 Alias mAChR M4; Muscarinic Acetylcholine Receptor M4; Cholinergic receptor muscarinic 4; Chrm 4; CHRM4; HM 4; HM4; M4; Muscarinic acetylcholine receptor M4; ACM4_HUMAN. literatures Specific References (1) | SL11998R has been referenced in 1 publications.[IF=1.642] Tong L et al. Effects of topical pilocarpine on ocular growth and refractive development in rabbits. Eur J Ophthalmol. 2020 Jun 11;1120672120934962. IHC-P&WB ; Rabbit.Research Area Cell biology Neurobiology Signal transduction The cell membrane受体 G protein-coupled receptor G protein signal Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Rabbit, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 53kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human CHRM4/mAChR M4: 1-31/479 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, mouse studies link its function to adenylyl cyclase inhibition. [provided by RefSeq, Jul 2008]
Function:
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine to these receptors and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and postassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, mouse studies link its function to adenylyl cyclase inhibition. Muscarinic acetylcholine receptor M4 has been reported to be expressed in many regions of the rat brain, particularly the striatum and hippocampus.
Subcellular Location:
Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
Similarity:
Belongs to the G-protein coupled receptor 1 family. Muscarinic acetylcholine receptor subfamily. CHRM4 sub-subfamily.
SWISS:
P08173
Gene ID:
1132
Database links:Entrez Gene: 1132 Human
Entrez Gene: 12672 Mouse
Omim: 118495 Human
SwissProt: P08173 Human
SwissProt: P32211 Mouse
Unigene: 248100 Human
Unigene: 330405 Mouse
Unigene: 10676 Rat
Product Picture Sample:
Lane 1: Human U-2 OS cell lysates
Lane 2: Human THP-1 cell lysates
Lane 3: Human MCF-7 cell lysates
Lane 4: Human A549 cell lysates
Primary: Anti-CHRM4 (SL11998R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 53 kD
Observed band size: 55 kD
Blank control (blue line): A431 cells(fixed with 70% ice-cold methanol overnight at 4℃).
Primary Antibody (green line): Rabbit Anti-CHRM4/PE-CY7 Conjugated antibody (SL11998R-PE-CY7),Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG-PE-CY7 .
Black line : Positive blank control A431); Negative blank control (Huvec)
Green line : Primary Antibody (Rabbit Anti-CHRM4 antibody (SL11998R) )
Orange line:Isotype Control Antibody (Rabbit IgG) .
Blue line : Secondary Antibody (Goat anti-rabbit IgG-AF488)
A431(Positive)and HUVEC Negative control)cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CHRM4 Antibody(SL11998R)at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody(blue) incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).Blank control: K562.
Primary Antibody (green line): Rabbit Anti-CHRM4 antibody (SL11998R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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