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Product Name NECAB1 Chinese Name 神经元钙结合相关蛋白EFCBP1抗体 Alias EF-CBP1; EF-hand calcium-binding protein 1; EFCBP1; N-terminal EF-hand calcium-binding protein 1; NECA1_HUMAN; Necab1; Neuronal calcium-binding protein 1; STIP-1. Research Area Cell biology Neurobiology Signal transduction Binding protein Immunogen Species Rabbit Clonality Polyclonal React Species Rat, (predicted: Human, Mouse, Dog, Pig, Horse, Rabbit, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 41kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human NECAB1/EF-CBP1: 51-150/351 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Members of the EF-CBP (N-terminal EF-hand calcium binding protein)/NECAB (neuronal calcium-binding protein) family participate in neuronal calcium signaling. EF-CBP1 (N-terminal EF-hand calcium binding protein 1), also known as STIP-1 or neuronal calcium-binding protein 1 (NECAB1), is a 351 amino acid cytoplasmic protein that contains one antibiotic biosynthesis monooxygenase (ABM) domain and two EF-hand domains. Expressed in brain, EF-CBP1 interacts with copine 6 and Syntaxin, and exists as two alternatively spliced isoforms. The gene encoding EF-CBP1 maps to human chromosome 8, which consists of nearly 146 million base pairs, encodes over 800 genes and is associated with a variety of diseases and malignancies. Schizophrenia, bipolar disorder, Trisomy 8, Pfeiffer syndrome, congenital hypothyroidism, Waardenburg syndrome and some leukemias and lymphomas are thought to occur as a result of defects in specific genes that map to chromosome 8.
Function:
Expressed in brain (at protein level).
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Expressed in brain
Similarity:
Contains 1 ABM domain.
Contains 2 EF-hand domains.
SWISS:
Q8N987
Gene ID:
64168
Database links:Entrez Gene: 64168 Human
SwissProt: Q8N987 Human
Unigene: 642655 Human
Unigene: 719466 Human
Product Picture Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-NECAB1 Polyclonal Antibody, Unconjugated(SL11978R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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