Product Name	SPOCK2
Chinese Name	睾丸蛋白聚糖2抗体
Alias	Kazal-like domains proteoglycan 2; CWCV; FLJ97039; KIAA0275; SPARC/osteonectin; Sparc/osteonectin cwcv and kazal like domains proteoglycan (testican) 2; Sparc/osteonectin cwcv and kazal like domains proteoglycan 2; SPOCK 2; Spock2; Testican 2; Testican-2; Testican2; TICN 2; TICN2; TICN2_HUMAN.
literatures	Specific References  (1)     |     SL11966R has been referenced in 1 publications. [IF=17.694] Xu An. et al. Rewired m6A epitranscriptomic networks link mutant p53 to neoplastic transformation. NAT COMMUN. 2023 Mar;14(1):1-19  IF ;  Mouse.   PubMed:36973285
Research Area	Cell biology  Neurobiology  Cytoskeleton  Extracellular matrix
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Horse, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	45kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human SPOCK2/Testican 2: 61-160/424
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a protein which binds with glycosaminoglycans to form part of the extracellular matrix. The protein contains thyroglobulin type-1, follistatin-like, and calcium-binding domains, and has glycosaminoglycan attachment sites in the acidic C-terminal region. Three alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Oct 2011] Function: May participate in diverse steps of neurogenesis. Binds calcium. Subcellular Location: Secreted. Tissue Specificity: Highly expressed in brain. Also found in lung and testis. Post-translational modifications: Contains chondroitin sulfate and heparan sulfate O-linked oligosaccharides. Similarity: Contains 1 Kazal-like domain. Contains 1 thyroglobulin type-1 domain. SWISS: Q92563 Gene ID: 9806 Database links: Entrez Gene: 9806 Human Omim: 607988 Human SwissProt: Q92563 Human Unigene: 523009 Human
Product Picture	Sample: Hela Cell (Human) Lysate at 40 ug Primary: Anti-SPOCK2 (SL11966R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 45 kD Observed band size: 50 kD Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPOCK2) Polyclonal Antibody, Unconjugated (SL11966R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SPOCK2) Polyclonal Antibody, Unconjugated (SL11966R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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