Product Name	PTF1A
Chinese Name	胰腺特异转录因子PTF1A抗体
Alias	PTF1-p48; bHLH transcription factor p48; bHLHa29; Class A basic helix-loop-helix protein 29; Class II bHLH protein PTF1A; Exocrine pancreas specific transcription factor p48; p48 DNA binding subunit of transcription factor PTF1; p48 DNA-binding subunit of transcription factor PTF1; Pancreas specific transcription factor 1a; Pancreas transcription factor 1 subunit alpha; Pancreas-specific transcription factor 1a; PTF 1A; PTF1 p48; PTF1-p48; Ptf1a; PTF1A_HUMAN; PTF1P48.
Research Area	Tumour  Cell biology  transcriptional regulatory factor  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Mouse, Rat,  (predicted: Human, Pig, Cow, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	35kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PTF1A: 165-260/328
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	PTF1 is a pancreas-specific protein that functions as a component of the trimeric pancreas transcription factor 1 (PTF1) complex. Localizing to the nucleus, PTF1 contains one basic helix-loop-helix (bHLH) domain and is believed to play an important role in mammalian pancreatic development, functioning as a transcription factor that regulates the specification of all three pancreatic cell types. PTF1 interacts with RBP-Jⅹ and, together, they cooperate in regulating the expression of PDX-1 (pancreas/duodenum homeobox protein 1), a key regulator of pancreatic islet development and insulin gene transcription in beta-cells. Loss of functional PTF1 can cause pancreatic progenitors to take on the normal fates of duodenal epithelia. Mutations in the gene encoding PTF1 lead to diabetes mellitus and cerebellar hypoplasia/agenesis, suggesting that PTF1 also plays and important role in cerebellar neurogenesis. Function: Transcriptional activator. Binds to the E-box consensus sequence 5'-CANNTG-3'. Plays an important role in determining whether cells allocated to the pancreatic buds continue towards pancreatic organogenesis or revert back to duodenal fates. May be involved in the maintenance of exocrine pancreas-specific gene expression including ELA1 and amylase. Required for the formation of pancreatic acinar and ductal cells. Plays an important role in cerebellar development. Subcellular Location: Nucleus. Cytoplasm. In chronic pancreatitis associated with pancreas cancer preferentially accumulates in the cytoplasm of acinar/ductular complexes. In the cytoplasm loses its ability to form the PTF1 complex. Tissue Specificity: Pancreas-specific (at protein level). Loss of expression is seen in ductal type pancreas cancers. DISEASE: Defects in PTF1A are the cause of diabetes mellitus and cerebellar hypoplasia/agenesis (DMCH) [MIM:609069]. Similarity: Contains 1 basic helix-loop-helix (bHLH) domain. SWISS: Q7RTS3 Gene ID: 256297 Database links: Entrez Gene: 256297 Human Entrez Gene: 19213 Mouse Entrez Gene: 117034 Rat Omim: 607194 Human SwissProt: Q7RTS3 Human SwissProt: Q9QX98 Mouse SwissProt: Q64305 Rat Unigene: 351503 Human Unigene: 69647 Mouse Unigene: 10536 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PTF1A) Polyclonal Antibody, Unconjugated (SL11930R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PTF1A) Polyclonal Antibody, Unconjugated (SL11930R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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