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Product Name Protein A Chinese Name 重组金黄色葡萄球菌蛋白A抗体 Alias Immunoglobulin G binding protein A; Staphylococcal protein A; SPA; gG-binding protein A; SPA_STAA8. literatures Specific References (3) | SL0362R has been referenced in 3 publications.[IF=9.518] Wu J et al. A laser-induced breakdown spectroscopy-integrated lateral flow strip (LIBS-LFS) sensor for rapid detection of pathogen. Biosens Bioelectron. 2019 Jul 13;142:111508. Other ; S. aureus.[IF=3.412] Lirui Ge. et al. Lateral Flow Immunoassay for Visible Detection of Human Brucellosis Based on Blue Silica Nanoparticles. Front Vet Sci. 2021; 8: 771341 Other ; Other.[IF=1.134] Wen Wu. et al. Ti3C2-MXene-assisted signal amplification for sensitive and selective surface plasmon resonance biosensing of biomarker. Chinese J Anal Chem. 2021 Nov;:Research Area immunology Immunogen Species Rabbit Clonality Polyclonal Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 40-60kDa Cellular localization Secretory protein Form Liquid Concentration 1mg/ml immunogen Recombinant protein A: full length Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Protein A is a 40-60 kDa surface protein originally found in the cell wall of the bacteria Staphylococcus aureus (SPA). SPA binds proteins from many of mammalian species, most notably IgGs, and helps inhibit phagocytic engulfment and acts as an immunological disguise via this type of interaction, thus the bacteria will disrupts opsonization and phagocytosis. SPA is known to bind with Fc region of immunoglobulins preferentially through interaction with the VH3 variable region of the heavy chain. SPA has been shown to bind with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b, whereas bind with moderate affinity to human IgM, IgA and IgE as well as mouse IgG3 and IgG1. SPA is often produced in E. coli and is practically coupled to other molecules such as enzymes, biotin, radioactive iodine for use in immunology and other biological research. SPA is also immobilized onto solid supports such as agarose beads for total IgG purifying or interest protein or protein complex identifying in immunoprecipitation studies.
Subcellular Location:
Secreted; cell wall.
SWISS:
P02976
Gene ID:
3919448
Database links:
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