Product Name	Histone H3 (Nuclear Loading Control)
Chinese Name	组蛋白H3（核内参）抗体
Alias	H3 histone family member E pseudogene; H3 histone family, member A; H3/A; H31_HUMAN; H3F3; H3FA; Hist1h3a; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; HIST3H3; histone 1, H3a; Histone cluster 1, H3a; Histone H3 3 pseudogene; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; H3.1; H3/d; H3C1; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C7; H3C8; H3FD;
literatures	Specific References  (32)     |     SL0349R has been referenced in 32 publications. [IF=8.56] Qian, Yi, et al. "Silver Nanoparticle-Induced Hemoglobin Decrease Involves Alteration of Histone 3 Methylation Status." Biomaterials (2015).  WB ;  Mouse.   PubMed:26295435 [IF=7.561] Zheng Hao. et al. Decreased Expression of Programmed Death Ligand-L1 by Seven in Absentia Homolog 2 in Cholangiocarcinoma Enhances T-Cell–Mediated Antitumor Activity. Front Immunol. 2022 Jan;0:138  WB ;  Human.   PubMed:35154166 [IF=7.243] Fanchun Zeng. et al. Antagonizing exosomal miR-18a-5p derived from prostate cancer cells ameliorates metastasis-induced osteoblastic lesions by targeting Hist1h2bc and activating Wnt/β-catenin pathway. GENES DIS. 2022 Jul;:  WB ;  Mouse.   PubMed:10.1016/j.gendis.2022.06.007 [IF=6.684] Zhao-Ming Xiao. et al. SMARCC1 Suppresses Tumor Progression by Inhibiting the PI3K/AKT Signaling Pathway in Prostate Cancer. Front Cell Dev Biol. 2021; 9: 678967  WB ;  Human.   PubMed:34249931 [IF=6.022] Huina Liu. et al. LncRNA, PLXDC2‐OT promoted the osteogenesis potentials of MSCs by inhibiting the deacetylation function of RBM6/SIRT7 complex and OSX specific isoform. 2021 Mar 08  WB ;  Human.   PubMed:33684230 [IF=6.02] Lee S et al. ChIP-seq analysis reveals alteration of H3K4 trimethylation occupancy in cancer-related genes by cold atmospheric plasma. Free Radic Biol Med. 2018 Oct;126:133-141.  WB ;  Human.   PubMed:30096431 [IF=4.75] Duan, Chao, et al. "Oestrogen receptor‐mediated expression of Olfactomedin 4 regulates the progression of endometrial adenocarcinoma." Journal of Cellular and Molecular Medicine (2014).  WB ;  Human.   PubMed:24495253 [IF=4.64] Zhang et al. Uncoupling Protein 2 Inhibits Myointimal Hyperplasia in Preclinical Animal Models of Vascular Injury. (2017) J.Am.Heart.Assoc. 6  WB ;  Mouse.   PubMed:29025747 [IF=4.486] Zhuohong Li. et al. Anlotinib exerts anti-cancer efficiency on lung cancer stem cells in vitro and in vivo through reducing NF-κB activity. 2021 May 06  WB ;  Mouse.   PubMed:33955683 [IF=4.427] Ji X et al. Histone modification in the lung injury and recovery of mice in response to PM2.5 exposure.. Chemosphere. 2019 Apr;220:127-136.  WB ;  Mouse.   PubMed:30579949 [IF=4.12] Joy, Marion, et al. "The Myocardin-related transcription factor MKL co-regulates the cellular levels of two profilin isoforms." Journal of Biological Chemistry (2017): jbc-M117.  WB ;  Human.   PubMed:28546428 [IF=3.91] Dong, Wei-tao, et al. "iTRAQ proteomic analysis of the interactions between Bombyx mori nuclear polyhedrosis virus and silkworm." Journal of Proteomics (2017).  WB ;  Other Species.   PubMed:28755911 [IF=3.906] Zhiwei Wang. et al. Dihydroartemisinin triggers ferroptosis in primary liver cancer cells by promoting and unfolded protein response‑induced upregulation of CHAC1 expression. Oncol Rep. 2021 Nov;46(5):1-14  WB ;  human.   PubMed:34558645 [IF=3.83] Yang et al. Celastrol Attenuates Multiple Sclerosis and Optic Neuritis in an Experimental Autoimmune Encephalomyelitis Model. (2017) Front.Pharmacol. 8:44  WB ;  Rat.   PubMed:28239352 [IF=3.701] Ying Sun. et al. Neuroprotective effects of natural cordycepin on LPS-induced Parkinson’s disease through suppressing TLR4/NF-κB/NLRP3-mediated pyroptosis. J Funct Foods. 2020 Dec;75:104274  WB ;  Mouse.   PubMed:10.1016/j.jff.2020.104274 [IF=3.639] Huiling Mao. et al. Leucine protects bovine intestinal epithelial cells from hydrogen peroxide-induced apoptosis by alleviating oxidative damage. J SCI FOOD AGR. 2022 May 05  WB ;  Bovine.   PubMed:35437753 [IF=3.448] Wang L et al. PHD2 exerts anti-cancer and anti-inflammatory effects in colon cancer xenografts mice via attenuating NF-κB activity. Life Sci. 2020 Feb 1;242:117167.  WB ;  Mouse.   PubMed:31838134 [IF=3.361] Wang J et al. PIM1 inhibitor SMI-4a attenuated lipopolysaccharide-induced acute lung injury through suppressing macrophage inflammatory responses via modulating p65 phosphorylation. Int Immunopharmacol. 2019 Aug;73:568-574.  WB ;  Mouse.   PubMed:31203114 [IF=3.22] Zhou et al. Epigallocatechin-3-gallate inhibits proliferation and migration of human colon cancer SW620 cells in vitro. (2012) Acta.Pharmacol.Sin. 33:120-6  WB ;  Human.   PubMed:22101170 [IF=3.076] Sogorkova,et al.Optimization of cell growth on palmitoyl-hyaluronan knitted scaffolds developed for tissue engineering applications.(2018) Journal of Biomedical Materials Research. Part A. 106:1488-1499.  IF(ICC) ;  Human.   PubMed:29377555 [IF=2.97] Liu, Donghui, et al. ʺNonenzymatic glycation of high‐density lipoprotein impairs its anti‐inflammatory effects in innate immunity.ʺ Diabetes/Metabolism Research and Reviews 28.2 (2012): 186-195.  WB ;  Human.   PubMed:21928330 [IF=2.68] Shen, Haitao, et al. "Epigallocatechin-3-gallate alleviates paraquat-induced acute lung injury and inhibits upregulation of toll-like receptors." Life Sciences (2016).  WB ;  Mouse.   PubMed:27890776 [IF=2.561] Peng X et al. Overexpression of modified human TRβ1 suppresses the growth of hepatocarcinoma SK-hep1 cells in vitro and in xenograft models.Mol Cell Biochem. 2018 Dec;449(1-2):207-218.  WB ;  Human.   PubMed:29679278 [IF=2.363] Wang Li. et al. Trilobatin Induces Apoptosis and Attenuates Stemness Phenotype of Acquired Gefitinib Resistant Lung Cancer Cells via Suppression of NF-κB Pathway. 2021 Apr 16  WB ;  Human.   PubMed:33860693 [IF=1.936] Cui Y et al. Loganin prevents BV‐2 microglia cells from Aβ1‐42‐induced inflammation via regulating TLR4/TRAF6/NF‐κB axis.(2019) Cell Biol Int.  WB ;  Mouse.   PubMed:30288860 [IF=1.895] Lin,et al.The CXCL12/CXCR4 axis promotes migration, invasion and EMT in human papillary thyroid carcinoma B-CPAP cells via NF-κB signaling.(2018) Biochemistry and Cell Biology. :.  WB ;  Human.   PubMed:29316404 [IF=1.88] Zhao, Hongyu, et al. "Glycyrrhizic Acid Attenuates Sepsis-Induced Acute Kidney Injury by Inhibiting NF-κB Signaling Pathway." Evidence-Based Complementary and Alternative Medicine (2015).  WB ;  Rat.   PubMed:26904148 [IF=1.664] Peng et al. Overexpressing modified human TRβ1 suppresses the proliferation of breast cancer MDA-MB-468 cells. (2018) Oncol.Lett. 16:785-792  WB ;  Human.   PubMed:29963146 [IF=1.652] Wang F et al. Gypenosides attenuate lipopolysaccharide-induced optic neuritis in rats. Acta Histochem. 2018 May;120(4):340-346.  WB ;  Rat.   PubMed:29559175 [IF=1.52] Tang, Z-X., et al. "Selective antegrade cerebral perfusion attenuating the TLR4/NF-κB pathway during deep hypothermia circulatory arrest in a pig model." Cardiology 128.3 (2014): 243-250.  WB ;  Pig.   PubMed:24819356
Product Type	Internal reference anti
Research Area	Chromatin and nuclear signals  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Pig, Cow, Rabbit, Fruit Fly, )
Applications	WB=1:10000-50000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	15kDa
Cellular localization	The nucleus
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Histone H3: 71-136/136
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Modulation of the chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. The N-terminal tail of core histones undergoes different posttranslational modifications including acetylation, phosphorylation and methylation. These modifications occur in response to cell signal stimuli and have a direct effect on gene expression. In most species, the histone H2B is primarily acetylated at lysines 5, 12, 15 and 20. Histone H3 is primarily acetylated at lysines 9, 14, 18 and 23. Acetylation at lysine 9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Function: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. H3 is deposited into chromatin exclusively through a DNA replication-coupled pathway that can be associated with either DNA duplication or DNA repair synthesis during meiotic homologous recombination. Subunit: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA. Interacts with GCN5, whereby H3S10ph increases histone-protein interactions. Interacts with PDD1 and PDD3. Subcellular Location: Nucleus. Chromosome. Note=Localizes to both the large, transcriptionally active, somatic macronucleus (MAC) and the small, transcriptionally inert, germ line micronucleus (MIC). Post-translational modifications: Phosphorylated to form H3S10ph. H3S10ph promotes subsequent H3K14ac formation by GCN5. H3S10ph is only found in the mitotically dividing MIC, but not in the amitotically dividing MAC. H3S10ph is correlated with chromosome condensation during mitotic or meiotic micronuclear divisions. Acetylation of histone H3 leads to transcriptional activation. H3K14ac formation by GCN5 is promoted by H3S10ph. H3K9acK14ac is the preferred acetylated form of newly synthesized H3. Acetylation occurs almost exclusively in the MAC. Methylated to form H3K4me. H3K4me is only found in the transcriptionally active MAC. Methylated to form H3K9me in developing MACs during conjugation, when genome-wide DNA elimination occurs. At this stage, H3K9me specifically occurs on DNA sequences being eliminated (IES), probably targeted by small scan RNAs (scnRNAs) bound to IES, and is required for efficient IES elimination. H3K9me is required for the interaction with the chromodomains of PDD1 and PDD3. The full-length protein H3S (slow migrating) is converted to H3F (fast migrating) by proteolytic removal of the first 6 residues. H3F is unique to MIC, and processing seems to occur regularly each generation at a specific point in the cell cycle. Similarity: Belongs to the histone H3 family. SWISS: P68431 Gene ID: 8350 Database links: Entrez Gene: 8350 Human Entrez Gene: 8351 Human Entrez Gene: 8352 Human Entrez Gene: 8353 Human Entrez Gene: 8354 Human Entrez Gene: 8355 Human Entrez Gene: 8356 Human Entrez Gene: 8357 Human Entrez Gene: 8358 Human Entrez Gene: 8968 Human Entrez Gene: 260423 Mouse Entrez Gene: 319148 Mouse Entrez Gene: 319149 Mouse Entrez Gene: 319150 Mouse Entrez Gene: 319151 Mouse Entrez Gene: 319152 Mouse Entrez Gene: 319153 Mouse Entrez Gene: 360198 Mouse Entrez Gene: 97908 Mouse Entrez Gene: 100364501 Rat Entrez Gene: 100365669 Rat Entrez Gene: 291159 Rat Entrez Gene: 314977 Rat Entrez Gene: 364716 Rat Entrez Gene: 679950 Rat Entrez Gene: 679994 Rat Entrez Gene: 680511 Rat Entrez Gene: 680599 Rat Entrez Gene: 682330 Rat Entrez Gene: 691496 Rat SwissProt: P68431 Human SwissProt: P84243 Human SwissProt: Q16695 Human SwissProt: Q6NXT2 Human SwissProt: Q71DI3 Human SwissProt: P68433 Mouse SwissProt: P84228 Mouse SwissProt: Q6LED0 Rat 组蛋白的基因非常保守，在亲缘关系较远的种属中，四种组蛋白(H2A、H2A、H3、H4)氨基酸序列都非常相似，如海胆组织H3的氨基酸序列与来自小牛胸腺的H3的氨基酸序列间只有一个氨基酸的差异，小牛胸腺的H3的氨基酸序列与豌豆的H3也很相似。组蛋白是The nucleus内的一种碱性核蛋白，抗组蛋白抗体即是以组蛋白为靶抗原的一种自身，是抗核抗体的一种。分子量：16-18KDa。主要与药物性红斑狼疮、系统性红斑狼疮、类风湿关节炎有关。
Product Picture	Sample: 293T(Human) Cell Lysate at 30 ug 293T(invent)(Human) Cell Lysate at 30 ug Primary: Anti-HIST3H3  (SL0349R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15 kD Observed band size: 15 kD Sample: Lane 1: Mouse Raw264.7 cell lysates Lane 2: Human Hela cell lysates Lane 3: Human SH-SY5Y cell lysates Lane 4: Human Molt-4 cell lysates Lane 5: Human THP-1 cell lysates Lane 6: Human 293T cell lysates Primary: Anti- Histone H3 (SL0349R) at 1/50000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 15 kDa Observed band size: 15 kDa Sample: Lane 1: NIH/3T3(Mouse) Cell Lysate at 30 ug Lane 2: MCF-7 (Human) Cell Lysate at 30 ug Lane 3: SiHa (Human) Cell Lysate at 30 ug Lane 4: U-2OS (Human) Cell Lysate at 30 ug Lane 5: MOLT-4 (Human) Cell Lysate at 30 ug Primary: Anti-Histone H3’HIST3H3 (SL0349R) at 1/1000 dilution Secon Cartpieces 1 1Delete 5 5Delete S SDelete R RDelete + +Delete 7 7Delete Delete Delete 1 1Delete 1 1Delete $ $Delete 0 0Delete 8 8Delete $ $Delete 0 0Delete 0 0Delete 1 1Delete H HDelete H HDelete Delete i iDelete i iDelete Delete 1 1Delete Delete 1 1Delete 1 1Delete 0 0Delete Delete 2 2Delete 1 1Delete 0 0Delete 0 0Delete 0 0Delete 0 0Delete 0 0Delete 0 0Delete 1 1Delete 1 1Delete Delete - -Delete - -Delete 0 0Delete Delete t tDelete 0 0Delete 0 0Delete 0 0Delete f fDelete Delete Delete Delete Delete Delete Delete Delete Delete Delete Delete Delete Delete Delete Delete 8 8Delete $ $Delete Delete 0 0Delete Delete Delete Delete R RDelete 5 5Delete A ADelete Totalgoods,subtotals:￥Checkout Bought notes(bought amounts latest0) No one bought this product Total 0 records, divided into1 pages First Prev Next Last User Comment(Total0User Comment Num) No comment Total 0 records, divided into1 pages First Prev Next Last Username： Anonymous user E-mail： Rank： Content： Verification code： Call us +86 571 56623320 Email [email protected] Address Room 1-315, Kongle Changqing Building, No. 160 Guangye Road,Gongshu District, Hangzhou City, Zhejiang Province, China Join Us with Support Share About Us Contact Us Privacy Policy Shipping Policy Payment Policy Quality Guarantee Return & Refund policy Download Product list Leave a message Please select message type Products inquiry (price, lead time, order, etc.) Products inquiry (technical inquiry) Suggestions on website optimization Other suggestions * To protect against spam, please pass the CAPTCHA test below. +86 571 56623320 [email protected] +86 18668110335 Scan Wechat Qrcode