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Rabbit Anti-VTG antibody
Rabbit Anti-VTG antibody
vitellogenin.
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Regular members: $334.4
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Details

Product Name VTG
Chinese Name 鱼、青鳉鱼卵黄蛋白原抗体
Alias vitellogenin.  
literatures
Specific References  (4)     |     SL0306R has been referenced in 4 publications.
[IF=3.216] Zheng Chen. et al. Effects of dietary protein level on the growth, reproductive performance, and larval quality of female yellow catfish (Pelteobagrus fulvidraco) broodstock. Aquacult Rep. 2022 Jun;24:101102  IHC ;  Yellow Catfish.  
[IF=2.705] Shui Y et al. Exploring potential proteins associated with cyclin B 3′ UTR in Procambarus clarkii oocytes. Biochemical and Biophysical Research Communications.2019 517(3): 458-462.  WB ;  broodstock.  
[IF=1.554] Xiong Zitao. et al. Expression and localization of vitellogenin genes (VTG) and receptor (VGR) in the gonad development of silver pomfret Pampus argenteus. J OCEANOL LIMNOL. 2023 May;:1-18  IHC ;  Fish.  
[IF=1.54] Zhang, Xiaozheng, et al. "Histopathological and estrogen effect of pentachlorophenol on the rare minnow (Gobiocypris rarus)." Fish Physiology and Biochemistry (2014): 1-12.  IHC-P ;  
Research Area Cell biology  immunology  
Immunogen Species Rabbit
Clonality Polyclonal
Applications ELISA=1:5000-10000 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Form Liquid
Concentration 1mg/ml
immunogen VTG protein: full length vitellogenin from Medaka serum 
Lsotype IgG
Purification affinity purified by Protein A
Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
Product Detail 卵黄蛋白原(VTG)是检测外源性化合物雌激素效应的敏感分子生物Maker,作为生物Maker可以很好地评价环境水体中污染物雌激素效应。
卵黄蛋白原(Vitellogenin,VTG)是由卵生脊椎动物肝细胞合成的一种复杂大分子蛋白,是由雌激素诱导在肝脏中表达的Lipoprotein—卵黄蛋白的前体。成熟雌鱼在体内雌激素17β-雌二醇的刺激下由肝脏合成,经过血液运输到达卵巢并加工成卵黄蛋白。雄鱼在正常情况下不合成这种蛋白,但受到雌激素(内源或外源)刺激也可合成这种蛋白,由于没有卵巢有效地清除VTG,雄鱼血液中VTG浓度会升高。因此,通过检测VTG含量可以反映外界化合物的类雌激素作用及作用效应水平。
目前水生生物VTG已作为水中环境雌激素污染的重要生物标志。用于检测VTG的方法很多,包括:免疫组织化学 ELISA法、色谱法、免疫印记、原位杂交等。

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