Product Name	His tag
Chinese Name	His tagTag anti
Alias	HisX6; 6His; His-Tag; 6 His epitope tag; Hexa His tag; HHHHHH epitope tag; HHHHHH tag; Histidine.
literatures	Specific References  (17)     |     SL0287R has been referenced in 17 publications. [IF=2.914]   WB ;  Pig.   PubMed:32649964 [IF=19.924] Yifan Yang. et al. A Versatile Platform for the Tumor-Targeted Intracellular Delivery of Peptides, Proteins, and siRNA. ADV FUNCT MATER. 2023 Apr;:2301011  WB ;  Human.   PubMed:10.1002/adfm.202301011 [IF=7.2] Zhiyan Hu. et al. CIP4 targeted to recruit GTP-Cdc42 involving in invadopodia formation via NF-κB signaling pathway promotes invasion and metastasis of CRC. Mol Ther-Oncolytics. 2022 Feb;:  WB ;  Strain（Bl21）.   PubMed:10.1016/j.omto.2022.02.023 [IF=4.38] Ma, Xiangyi, et al. "DT390-triTMTP1, a novel fusion protein of diphtheria toxin with tandem repeat TMTP1 peptide, preferentially targets metastatic tumors." Molecular pharmaceutics 10.1 (2013): 115-126.  IHC-P ;   PubMed:23198829 [IF=4.235] Xu Zhenshang. et al. Comparison of Enzyme Secretion and Ferulic Acid Production by Escherichia coli Expressing Different Lactobacillus Feruloyl Esterases. Front Microbiol. 2020 Nov;11:2281  WB ;   PubMed:33329424 [IF=3.309] Chuandong Cheng. et al. SREBP2/Rab11s/GLUT1/6 network regulates proliferation and migration of glioblastoma. PATHOL RES PRACT. 2022 Oct;:154176  WB ;  Human.   PubMed:36327817 [IF=2.984] Ruirui Luoet al. Clostridium perfringens beta2 toxin induced in vitro oxidative damage and its toxic assessment in porcine small intestinal epithelial cell lines. Gene . 2020 Oct 30;759:144999.  WB ;  pig.   PubMed:32717305 [IF=2.984]   WB, IF ;  Pig.   PubMed:32717305 [IF=2.914] Xiaoli Gao. et al. Effects of Clostridium perfringens beta2 toxin on apoptosis, inflammation, and barrier function of intestinal porcine epithelial cells. Microb Pathogenesis. 2020 Oct;147:104379  WB ;  Pig.   PubMed:32649964 [IF=2.913] Li N et al. Identification and characterization of an atypical RIG-I encoded by planarian Dugesia japonica and its essential role in the immune response.(2019) Dev Comp Immunol. Feb;91:72-84.  WB ;   PubMed:30355517 [IF=2.7] Peng, Wang, et al. "Attenuation of specific CTL responses by highly efficient transduction of the recombinant adenovirus expressing His-tag-ICP47 fusion gene." Life Science Journal 10.1 (2013).  WB ;   PubMed:Attenuation of specific CTL responses by highly efficient transduction of the recombinant adenovirus expressing His-tag-ICP47 fusion gen [IF=2.7] WANG, Peng, et al. "Construction and identification of a recombinant adenovirus vector expressing His-tag-ICP47 fusion gene." Life Science Journal 9.1 (2012).   PubMed:Life Science Journal 9.1 (2012) [IF=2.56] Wang, P. et al. "Attenuation of cytokine production of interleukine -2 and perforin in Two-way mixed lymphocyte reaction by highly efficient transduction of the recombinant adenovirus expressing ICP47 gene."Life Sci J 2013;10(3):963-969  WB ;   PubMed:ISSN:1097-8135 [IF=1.74] Wang, Peng, et al. "Recombinant adenovirus expressing ICP47 gene suppresses the ability of dendritic cells by restricting specific T cell responses." Cellular Immunology (2013).  WB ;   PubMed:23774531 [IF=1.676] Ma et al. Adjuvant effects mediated by the carbohydrate recognition domain of Agrocybe aegerita lectin interacting with avian influenza H9N2 viral surface glycosylated proteins. (2017) J.Zhejiang.Univ.Sci.. 18:653-661  other ;   PubMed:28786240 [IF=1.109] He,et al.Recombinant Fiber-2 Protein-Based Indirect ELISA for Antibody Detection of Fowl Adenovirus Serotype 4.(2018) Avian Diseases. 62:73-78.  WB ;   PubMed:29620471 [IF=0] Ren et al. Long-term rescue of rat retinal ganglion cells and visual function by AAV-mediated BDNF expression after acute elevation of intraocular pressure. (2012) Invest.Ophthalmol.Vis.Sci. 53:1003-11  IHC ;   PubMed:22247466
Product Type	Tag anti
Research Area	Tumour  Cell biology  immunology  Chromatin and nuclear signals  Signal transduction  Synthesis and Degradation  Binding protein
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Recombinant protein
Applications	WB=1:1000-5000  not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Form	Liquid
Concentration	1mg/ml
immunogen	Synthetic peptide: HHHHHH conjugated to KLH
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The H-H-H-H-H-H motif is used as a tag on many recombinant proteins to facilitate purification. The antibody recognizes the His-tag fused to the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. SWISS: N/A Gene ID: N/A Tag anti 蛋白质纯化的新途径─应用His-Tag抗体检测His-Tag融合蛋白 1） 6xHis与不同的Sepharose纯化填料结合（捆绑），可不受构象的影响，在天然或变形的条件下可进行进一步纯化。 2） 可在温和的洗脱液环境中，其结合、洗涤及洗脱步骤不会对蛋白结构产生影响。 3） 6xHis标签比普通（Tag）要小得多，经纯化后的蛋白可直接进行下游操作。可用于任何表达系统（包括： 细菌，杆状病毒和哺乳动物） 4） 6xHis标签在生理溶液pH下不带电荷，其标签不会干涉重组蛋白的结构和功能， 6xHis不会影响蛋白分泌 5） 6xHis标签免疫原性差，重组蛋白可以不必除去标签而直接作为抗原进行免疫
Product Picture	Sample: Lane1: His-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug Lane2: His-Tagged Fusion Protein Overexpression E.coli Lysate at 2ug Primary: Anti-His tag (SL0287R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 41 kD Observed band size: 45 kD Sample: Lane 1: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41403P) at 2ug Lane 2: His-Tagged Fusion Protein Overexpression E.coli Lysate (Cat#: SL41403P) at 4ug Primary: Anti-His tag (SL0287R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 51 kD Observed band size: 51 kD

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