Product Name	PLGF
Chinese Name	胎盘生长因子抗体
Alias	Placenta growth factor; PGF; PGFL; PIGF; Placental growth factor vascular endothelial growth factor related protein; PlGF 2; PLGF; PlGF2; PLGF_HUMAN; Vascular endothelial growth factor related protein.
Research Area	Tumour  Cell biology  immunology  Growth factors and hormones  transcriptional regulatory factor  vascular endothelial cell
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	23kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PLGF: 166-221/221
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The placental-derived growth factor (PIGF) is a dimeric glycoprotein showing a high degree of sequence similarity to the vascular endothelial growth factor. Alternative splicing of the PIGF primary transcript gives rise to two forms, named PIGF-1 and PIGF-2, which differ only in the insertion of a highly basic 21-amino acid stretch at the carboxyl end. The presence of the PIGF mRNA in thyroid, placenta, lung, and goiter has indicated the tissues where this factor functions. However, the role of PIGF in vascular development has not yet been clearly established. Function: Growth factor active in angiogenesis and endothelial cell growth, stimulating their proliferation and migration. It binds to the receptor FLT1/VEGFR-1. Isoform PlGF-2 binds NRP1/neuropilin-1 and NRP2/neuropilin-2 in a heparin-dependent manner. Subunit: Antiparallel homodimer; disulfide-linked. Also found as heterodimer with VEGFA/VEGF. Isoform PlGF-3 is found both as homodimer and as monomer. Subcellular Location: Secreted. The three isoforms are secreted but PlGF-2 appears to remain cell attached unless released by heparin. Tissue Specificity: While the three isoforms are present in most placental tissues, PlGF-2 is specific to early (8 week) placenta and only PlGF-1 is found in the colon and mammary carcinomas. Post-translational modifications: N-glycosylated. Similarity: Belongs to the PDGF/VEGF growth factor family. SWISS: P49763 Gene ID: 5228 Database links: Entrez Gene: 5228 Human Entrez Gene: 18654 Mouse Omim: 601121 Human SwissProt: P49763 Human SwissProt: P49764 Mouse Unigene: 252820 Human Unigene: 4809 Mouse PLGF胎盘生长因子是血管生长因子家族成员之一，是动脉粥样硬化斑块不稳定的主要炎性激活物，因此它可作为ACS患者风险预测的生物学Maker.尿胎盘生长因子水平可预测先兆子痫危险.研究人员发现，在疾病发作前的6到8周，先兆子痫患者尿液中的胎盘生长因子水平急剧下降,这种新的尿检方法使提前1到2个月预测先兆子痫成为可能。亦有研究人员正在从事胎盘生长因子与肺气肿，胃癌，肠癌等方面的研究。 相关抗体 SL0280R(抗大鼠) SL0234R(抗小鼠)
Product Picture	Sample: SW480(Human) Cell Lysate at 30 ug Primary: Anti-PLGF (SL0281R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 29 kD Paraformaldehyde-fixed, paraffin embedded (Human stomach carcer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Placenta growth factor; PLGF) Polyclonal Antibody, Unconjugated (SL0281R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: human liver carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PLGF Polyclonal Antibody, Unconjugated(SL0281R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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