Product Name	Visfatin
Chinese Name	内脂素/内脏脂肪素/前B细胞克隆增强因子1抗体
Alias	pre-B-cell colony-enhancing factor 1; PBEF1 protein; AI480535; 1110035O14Rik; AI314458; DKFZP666B131; EC 2.4.2.12; MGC117256; NAmPRTase; NAMPT; Nicotinamide phosphoribosyltransferase; PBEF; PBEF1; Pre B cell colony enhancing factor 1; Pre B cell colony enhancing factor; Pre B cell enhancing factor; Visfatin; NAMPT_HUMAN.
literatures	Specific References  (2)     |     SL0272R has been referenced in 2 publications. [IF=5.032] Cai, Bolin. et al. circPTPN4 regulates myogenesis via the miR-499-3p/NAMPT axis. J Anim Sci Biotechno. 2022 Dec;13(1):1-15  WB ;  Chicken.   PubMed:35152912 [IF=1.77] Lin, Li-Wen, et al. "Visfatin/pre-B cell colony-enhancing factor immunohistochemical overexpression in oral cancers." Journal of Applied Biomedicine (2014).  IHC-P ;  Human.   PubMed:10.1016/j.jab.2014.08.003
Research Area	Cardiovascular  Neurobiology  Diabetes  Endocrinopathy
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Pig, Cow, Horse, Monkey, )
Applications	WB=1:500-2000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	55kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PBEF: 301-400/491
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011]. Function: Catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, an intermediate in the biosynthesis of NAD. It is the rate limiting component in the mammalian NAD biosynthesis pathway. Subunit: Homodimer. Subcellular Location: Cytoplasm. Tissue Specificity: Expressed in large amounts in bone marrow, liver tissue, and muscle. Also present in heart, placenta, lung, and kidney tissues. Similarity: Belongs to the NAPRTase family. SWISS: P43490 Gene ID: 10135 Database links: Entrez Gene: 10135 Human Entrez Gene: 59027 Mouse Entrez Gene: 297508 Rat Omim: 608764 Human SwissProt: P43490 Human SwissProt: Q99KQ4 Mouse SwissProt: Q80Z29 Rat Unigene: 489615 Human Unigene: 202727 Mouse Unigene: 203508 Rat 一种新的脂肪因子-visfatin是新近发现的主要由人和小鼠内脏脂肪组织分泌的一种脂肪cell factor,其结构与pre-B细胞集落增强因子相似.它能够发挥类似胰岛素的作用,与Ⅱ型Diabetes相关联,降低血糖,促进糖摄取,可结合并活化胰岛素受体,激活胰岛素信号通路.内脏脂肪因子-Visfatin与肥胖密切相关并能够促进脂肪细胞的分化,还能促进血管平滑肌细胞成熟. Visfatin的表达受炎症反应因子和多种激素的调节.PBEF-1可能是联系机体糖脂代谢的重要分子,它的发现可为揭示Diabetes与肥胖的发生发展机制提供新的研究思路,为代谢综合征的治疗提供新方案.
Product Picture	Sample: Heart (Mouse) Lysate at 40 ug Primary: Anti-Visfatin (SL0272R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD Sample: Lane1:Brain(Rat) Lysate at 30 ug Lane2:Liver (Rat) Lysate at 30 ug Primary: Anti-VISFATIN-1 (SL0272R) at 1:200 dilution Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 dilution Predicted band size : 55kD Observed band size : 54kD Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Visfatin) Polyclonal Antibody, Unconjugated (SL0272R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti- Visfatin Polyclonal Antibody, Unconjugated(SL0272R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti- Visfatin Polyclonal Antibody, Unconjugated(SL0272R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-Visfatin antibody (SL0272R) Dilution: 1ug/Test; Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5ug/Test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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