Product Name	BDNF
Chinese Name	脑源神经营养因子抗体
Alias	Abrineurin; Brain Derived Neurotrophic Factor; MGC34632; Neurotrophin; BDNF_HUMAN.
literatures	Specific References  (16)     |     SL0248R has been referenced in 16 publications. [IF=7.59] Yuanxin Zhai. et al. High-efficiency Brain-targeted Intranasal Delivery of BDNF Mediated by Engineered Exosomes to Promote Remyelination. BIOMATER SCI-UK. 2022 Aug;:  IF ;  Mouse.   PubMed:36039673 [IF=5.546] Min Ai. et al. 1,25(OH)2D3 attenuates sleep disturbance in mouse models of Lewis lung cancer, in silico and in vivo. 2021 Jun 01  WB ;  Mouse.   PubMed:34061988 [IF=4.94] Li, Hui, et al. "Therapeutic potential of in utero mesenchymal stem cell (MSCs) transplantation in rat foetuses with spina bifida aperta." Journal of cellular and molecular medicine 16.7 (2012): 1606-1617.  IHC-P ;  Rat.   PubMed:22004004 [IF=4.562] Seo JH et al. Physical exercise ameliorates psychiatric disorders and cognitive dysfunctions by hippocampal mitochondrial function and neuroplasticity in post-traumatic stress disorder. Experimental Neurology, 2019,322,113043.  WB ;  Rat.   PubMed:doi:10.1016/j.expneurol.2019.113043 [IF=4.366] Giulia Federica Mancini. et al. Social Defeat Stress during Early Adolescence Confers Resilience against a Single Episode of Prolonged Stress in Adult Rats. Cells-Basel. 2021 Feb;10(2):360  WB ;  Rat.   PubMed:33572375 [IF=3.37] Lu-Lu Xue. et al. BDNF promotes neuronal survival after neonatal hypoxic-ischemic encephalopathy by up-regulating Stx1b and suppressing VDAC1. Brain Res Bull. 2021 Sep;174:131  WB ;  Rat.   PubMed:34058286 [IF=3.244] Fu XM et al. The Combination of Adipose-derived Schwann-like Cells and Acellular Nerve Allografts PromotesSciatic Nerve Regeneration and Repair through the JAK2/STAT3 Signaling Pathway in Rats. Neuroscience. 2019 Nov 1;422:134-145.  ICF&WB ;  Rat.   PubMed:31682951 [IF=3.105] Huan-Tong Wu. et al. Edaravone attenuates H2O2 or glutamate-induced toxicity in hippocampal neurons and improves AlCl3/D-galactose induced cognitive impairment in mice. Neurotoxicology. 2021 Jul;85:68  WB ;  Rat.   PubMed:34004234 [IF=3.103] Li R et al. Lithium chloride promoted hematoma resolution after intracerebral hemorrhage through GSK-3β-mediated pathways-dependent microglia phagocytosis and M2-phenotype differentiation, angiogenesis and neurogenesis in a rat model. Brain Res Bull. 2019 Jul 17;152:117-127.  WB ;  Rat.   PubMed:31325596 [IF=2.977] Xiaonan Zhang. et al. The involvement of ADAR1 in antidepressant action by regulating BDNF via miR-432. Behav Brain Res. 2021 Mar;402:113087  WB,IF ;  Mouse.   PubMed:33412228 [IF=2.733] Zhai X et al. L-lactate preconditioning promotes plasticity-related proteins expression and reduces neurological deficits by potentiating GPR81 signaling in rat traumatic brain injury model. Brain Res. 2020 Nov 1;1746:146945.  WB&IHF ;  Rat.   PubMed:32531223 [IF=2.63] Yang Tingting. et al. The 5-HT and PLC Signaling Pathways Regulate the Secretion of IL-1β, TNF-α and BDNF from NG2 Cells. EVID-BASED COMPL ALT. 2022;2022:7425538  WB ;  Rat.   PubMed:35600957 [IF=2.629] Zhao Ying. et al. Electroacupuncture Alleviates Visceral Hypersensitivity in IBS-D Rats by Inhibiting EGCs Activity through Regulating BDNF/TrkB Signaling Pathway. Evid-Based Compl Alt. 2022;2022:2497430  IF ;  Rat.   PubMed:10.1155/2022/2497430 [IF=2.514] Tianshu Wang. et al. Low-Intensity Pulsed Ultrasound Prompts Both Functional and Histologic Improvements While Upregulating the Brain-Derived Neurotrophic Factor Expression after Sciatic Crush Injury in Rats. Ultrasound Med Biol. 2021 Mar;:  IHC ;  Rat.   PubMed:33745752 [IF=1.709] Sunariani J et al. Difference of brain-derived neurotrophic factor expression and pyramid cell count during mastication of food with varying hardness. J Appl Oral Sci. 2019 Apr 1;27:e20180182.  IHF-P ;  Rat.   PubMed:30970112 [IF=1.177]  Zhenting Huang . et al. Anti-Cognitive Decline by Yinxing-Mihuan-Oral-Liquid via Activating CREB/BDNF Signaling and Inhibiting Neuroinflammatory Process. 2021 Jan 27  IF,IHC ;  Rat.   PubMed:33499761
Research Area	Neurobiology  Signal transduction  Growth factors and hormones  transcriptional regulatory factor  Kinases and Phosphatases
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat, Rabbit,  (predicted: Mouse, Dog, Pig, Cow, Horse, Guinea Pig, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	13/27kDa
Cellular localization	The nucleus Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human BDNF: 81-180/247
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Neurotrophins function to regulate naturally occurring cell death of neurons during development. The prototype neurotrophin is nerve growth factor (NGF), originally discovered in the 1950s as a soluble peptide promoting the survival of, and neurite outgrowth from, sympathetic ganglia. More recently, three additional structurally homologous neurotrophic factors have been identified. These include brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3) and neurotrophin-4 (NT-4), also designated NT-5. These various neurotrophins stimulate the in vitro survival of distinct but partially overlapping populations of neurons. The Trk A receptor is the preferential receptor for NGF, but also binds NT-3 and NT-4. The Trk B receptor binds equally well to both BDNF and NT-4 and to a lesser extent NT-3, while the Trk C receptor only binds NT-3. BDNF promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Belongs to the NGF-beta family. Function: During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. Subunit: Monomers and homodimers. Binds to NTRK2/TRKB. Subcellular Location: Secreted. Tissue Specificity: Brain. Highly expressed in hippocampus, amygdala, cerebral cortex and cerebellum. Also expressed in heart, lung, skeletal muscle, testis, prostate and placenta. Post-translational modifications: The propeptide is N-glycosylated and glycosulfated. Converted into mature BDNF by plasmin (PLG). DISEASE: Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. Similarity: Belongs to the NGF-beta family. SWISS: P23560 Gene ID: 627 Database links: Entrez Gene: 627 Human Entrez Gene: 12064 Mouse Entrez Gene: 24225 Rat Omim: 113505 Human SwissProt: P23560 Human SwissProt: P21237 Mouse SwissProt: P23363 Rat Unigene: 502182 Human Unigene: 1442 Mouse Unigene: 11266 Rat Neurobiology相关蛋白（Neurobiology） 人脑源性神经营养因子(BDNF)和神经营养素-3(NT-3)神经生长因子(NGF)属于神经营养因子(NTF)家族的组成成员，具有促进早期神经元的增殖与分化，维持成熟神经元的存活的生物学功能,能调控神经元再生的微环境、可促进运动神经元的存活和生长并能防止它受损及死亡。对治疗神经系统疾病有广泛的临床应用前景。 目前已经用于某些疾病的临床试验：如早期老年性痴呆症、帕金森氏症、Diabetes、艾滋病或其他原因引起的外周神经损伤等研究。
Product Picture	Sample: Brain(Rat)lysate at 30ug; Heart(Rat)lysates, 30ug Primary: Anti-BDNF (SL0248R) at 1:200 Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000 Predicted band size : 13kD,25kD Observed band size : 25kD Tissue/cell: rabbit brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-BDNF Polyclonal Antibody, Unconjugated(SL0248R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-BDNF Polyclonal Antibody, Unconjugated(SL0248R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: HepG2(blue). Primary Antibody:Rabbit Anti-BDNF antibody (SL0248R,Green); Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA; Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions; Secondary Antibody: Goat anti-rabbit IgG-FITC(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA. Protocol The cells were fixed with 2% paraformaldehyde for 10 min at 37℃. Primary antibody (SL0248R, 1μg /1x10^6 cells) were incubated for 30 min at room temperature, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/FITC antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 40 min at room temperature. Acquisition of 20,000 events was performed.

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