Product Name	IGF1R
Chinese Name	胰岛素样生长因子I受体抗体
Alias	Insulin-like growth factor 1 receptor; CD221; CD221 antigen; IGF1 Receptor; IGF 1 receptor; IGF 1R; IGF I receptor; IGFIRC; Insulin like growth factor 1 receptor precursor; JTK13; MGC142170; MGC142172; Insulin-like growth factor 1 receptor beta chain; IGF 1 receptor; IGF-1R; IGF I receptor; IGF-I receptor; IGF-IR; IGF1R_HUMAN.
literatures	Specific References  (11)     |     SL0227R has been referenced in 11 publications. [IF=20.693] Pei Dai. et al. Gimap5 promoted RSV degradation through interaction with M6PR. J MED VIROL. 2022 Dec;:  WB ;  Human.   PubMed:36484389 [IF=9.429] Xu, Kaiwei. et al. IGF1 receptor-targeted black TiO2 nanoprobes for MRI-guided synergetic photothermal-chemotherapy in drug resistant pancreatic tumor. J NANOBIOTECHNOL. 2022 Dec;20(1):1-17  WB ;  Human.   PubMed:35794573 [IF=6.304] Xuemei Shen. et al. CircRILPL1 promotes muscle proliferation and differentiation via binding miR-145 to activate IGF1R/PI3K/AKT pathway. Cell Death Dis. 2021 Feb;12(2):1-14  WB ;  Bovine.   PubMed:33542215 [IF=4.711] Yurie Yamamoto. et al. Establishment of a gastric cancer cell line with high microsatellite instability, OCUM-13, derived from Borrmann type-2 primary tumor. CANCER MED-US. 2022 Nov;:  IHC ;  Human.   PubMed:36324252 [IF=2.784] Liu et al. Ghrelin protects the myocardium with hypoxia/reoxygenation treatment through upregulating the expression of growth hormone, growth hormone secretagogue receptor and insulin-like growth factor-1, and promoting the phosphorylation of protein kinase B. (2018) Int.J.Mol.Med. 42:3037-3046  WB&IHC ;  Rat.   PubMed:30272367 [IF=2.564] Balogh O et al. Expression of insulin-like growth factor 1 and its receptor in preovulatory follicles and in the corpus luteum in the bitch. Gen Comp Endocrinol. 2018 Dec 1;269:68-74.  IHC-P ;  Dog.   PubMed:30125572 [IF=2.38] Yang, Shaobo, et al. "Insulin-Like Growth Factor-1 Modulates Polycomb Cbx8 Expression and Inhibits Colon Cancer Cell Apoptosis." Cell Biochemistry and Biophysics (2014): 1-5.  WB ;  Human.   PubMed:25398592 [IF=2.19] Hu, Wei, et al. "MicroRNA let-7a and let-7f as novel regulatory factors of the sika deer (Cervus nippon) IGF-1R gene." Growth Factors 0 (2013): 1-7.  WB ;   PubMed:24294913 [IF=2.092] Yan Chunchi. et al. Increase of E3 ubiquitin ligase NEDD4 expression leads to degradation of its target proteins PTEN/IGF1R during the formation of goose fatty liver. J Anim Sci. 2020 Sep;98(9):  WB ;  Goose.   PubMed:32841331 [IF=1.422] Muller et al. Different expression of leptin and IGF1 in the adult and prepubertal testis in dogs. (2017) Reprod.Domest.Anim. 52 Suppl 2:187-192  IHC ;  Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep,.   PubMed:28101891 [IF=1.21] Müller, L., et al. "Different expression of leptin and IGF1 in the adult and prepubertal testis in dogs." Reproduction in Domestic Animals (2017).  IHC-P ;  Dog.   PubMed:10.1111/rda.12896
Research Area	Tumour  immunology  Signal transduction  Growth factors and hormones  transcriptional regulatory factor  Kinases and Phosphatases  The cell membrane受体  Diabetes
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Dog,  (predicted: Rat, Pig, Cow, Horse, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	78/150kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human IGF-1R alpha chain: 251-350/1367
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. [provided by RefSeq, Jul 2008]. Function: This receptor binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. It has a tyrosine-protein kinase activity, which is necessary for the activation of the IGF1-stimulated downstream signaling cascade. When present in a hybrid receptor with INSR, binds IGF1. PubMed:12138094 shows that hybrid receptors composed of IGF1R and INSR isoform Long are activated with a high affinity by IGF1, with low affinity by IGF2 and not significantly activated by insulin, and that hybrid receptors composed of IGF1R and INSR isoform Short are activated by IGF1, IGF2 and insulin. In contrast, PubMed:16831875 shows that hybrid receptors composed of IGF1R and INSR isoform Long and hybrid receptors composed of IGF1R and INSR isoform Short have similar binding characteristics, both bind IGF1 and have a low affinity for insulin. Subunit: Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand-binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. Forms a hybrid receptor with INSR, the hybrid is a tetramer consisting of 1 alpha chain and 1 beta chain of INSR and 1 alpha chain and 1 beta chain of IGF1R. Interacts with ARRB1 and ARRB2. Subcellular Location: Membrane; Single-pass type I membrane protein. Tissue Specificity: Found as a hybrid receptor with INSR in muscle, heart, kidney, adipose tissue, skeletal muscle, hepatoma, fibrobasts, spleen and placenta (at protein level). Expressed in a variety of tissues. Post-translational modifications: The cytoplasmic domain of the beta subunit is autophosphorylated on tyrosine residues in response to insulin-like growth factor I (IGF I). Phosphorylation of Tyr-980 is required for IRS1- and SHC1-binding. DISEASE: Defects in IGF1R may be a cause in some cases of resistance to insulin-like growth factor 1 (IGF1 resistance) [MIM:270450]. IGF1 resistance is a gowth deficiency disorder characterized by intrauterine growth retardation and poor postnatal growth accompanied with increased plasma IGF1. Similarity: Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. Contains 3 fibronectin type-III domains. Contains 1 protein kinase domain. SWISS: P08069 Gene ID: 3480 Database links: Entrez Gene: 3480 Human Entrez Gene: 16001 Mouse Entrez Gene: 25718 Rat Omim: 147370 Human SwissProt: P08069 Human SwissProt: Q60751 Mouse SwissProt: P24062 Rat Unigene: 643120 Human Unigene: 714012 Human Unigene: 275742 Mouse Unigene: 10957 Rat
Product Picture	Blank control: MCF7. Primary Antibody (green line): Rabbit Anti-IGF1R antibody (SL0227R) Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Positive control: (mo)Splenocytes(2% Paraformaldehyde-fixed ) Isotype Control Antibody Antibody: Rabbit IgG; Supplier: Cell Signaling Technology; Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA Secondary Antibody Antibody: Goat anti-rabbit IgG-FITC; Supplier: Cell Signaling Technology; Dilution: 1:200 in 1 X PBS containing 0.5% BSA Primary Antibody Supplier: Bioss; Supplier catalog number: SL0227R; Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA

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