Product Name	DFFA
Chinese Name	DFFA抗体
Alias	DNA fragmentation factor subunit alpha; DFF-45; Inhibitor of CAD; ICAD; DFF1; DFF45; H13; DFFA; DFFA_HUMAN;
Research Area	Cell biology  immunology  Chromatin and nuclear signals  Apoptosis  The new supersedes the old  Epigenetics
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	36kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human ICAD: 2-100/331
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Caspase-activated deoxyribonuclease(CAD) also designated DNA fragmentation factor 45 kDa subunit (DFF-45)and Caspase-activated DNase. CAD is a nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. ICAD (DNA fragmentation factor alpha subunit) (DNA fragmentation factor 45 kDa subunit) (DFF-45) (Inhibitor of CAD) is inhibitor of the caspase-activated DNase (DFF44). Function: Inhibitor of the caspase-activated DNase (DFF40). Subunit: Heterodimer of DFFA and DFFB. Subcellular Location: Cytoplasm. Post-translational modifications: Caspase-3 cleaves DFF45 at 2 sites to generate an active factor. Similarity: Contains 1 CIDE-N domain. SWISS: O54786 Gene ID: 1676 Database links: Entrez Gene: 1676 Human Omim: 601882 Human SwissProt: O00273 Human Unigene: 484782 Human ICAD 是在Apoptosis期诱导DNA分裂和染色体固缩的一种核酶，降解裸露的DNA 并诱导形态学变化。
Product Picture	Sample: liver (mouse) Lysate at 40 ug lung(mouse) Lysate at 40 ug BRL-3A (rat)cell Lysate at 40 ug Primary: Anti- ICAD (SL0214R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36kD Observed band size: 36 kD Sample: A431 Cell (Human) Lysate at 40 ug Primary: Anti-ICAD (SL0214R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 36 kD Observed band size: 36 kD Tissue/cell: Human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ICAD Polyclonal Antibody, Unconjugated(SL0214R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ICAD/DFF-45 Beta Polyclonal Antibody, Unconjugated(SL0214R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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