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Product Name Big endothelin-1 Chinese Name 内皮素1抗体 Alias EDN1_HUMAN; Big endothelin 1; EDN 1; EDN1; EDN1 protein; Endothelin1; Endothelin 1; Endothelin-1; ET 1; ET1; OTTHUMP00000039245; PPET1; Preproendothelin 1; Preproendothelin1. literatures Specific References (1) | SL0202R has been referenced in 1 publications.[IF=3.647] Juan Wang. et al. NR4A3 induces endothelial dysfunction through up-regulation of endothelial 1 expression in adipose tissue-derived stromal cells. Life Sci. 2021 Jan;264:118727 IF ; Human.Research Area Cardiovascular immunology Neurobiology Immunogen Species Rabbit Clonality Polyclonal React Species Human, (predicted: Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, ) Applications ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 22kDa Cellular localization Secretory protein Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Big endothelin-1: 81-212/212 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms - ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver. Endothelin 1 is expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells.
Function:
Endothelins are endothelium-derived vasoconstrictor peptides.
Subcellular Location:
Secreted.
Tissue Specificity:
Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells.
Similarity:
Belongs to the endothelin/sarafotoxin family.
SWISS:
P05305
Gene ID:
1906
Database links:Entrez Gene: 1906 Human
Entrez Gene: 13614 Mouse
Omim: 131240 Human
SwissProt: P05305 Human
SwissProt: P22387 Mouse
Unigene: 511899 Human
Unigene: 713645 Human
Unigene: 10918 Rat
内皮素(Endothelin-1,ET)是一种强的血管收缩剂,分为ET-1,ET-2,ET-3 三种,ET-1主要在endothelial cells中表达,而ET-2和ET-3则主要在神经系统中表达。
ET具有广泛的生物学作用.Product Picture Tissue/cell: human gastric mucosa; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ET-1 Polyclonal Antibody, Unconjugated(SL0202R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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