Product Name	Big endothelin-1
Chinese Name	内皮素-1抗体
Alias	EDN1_HUMAN; Big endothelin 1; EDN 1; EDN1; EDN1 protein; Endothelin1; Endothelin 1; Endothelin-1; ET 1; ET1; OTTHUMP00000039245; PPET1; Preproendothelin 1; Preproendothelin1.
literatures	Specific References  (1)     |     SL0188R has been referenced in 1 publications. [IF=3.164] Riggs JL et al. Intermittent Hypoxia Exacerbates Increased Blood Pressure in Rats with Chronic Kidney Disease JL Riggs, CE Pace, HH Ward, LV Gonzalez Bosc… - American Journal of …, 2018Am J Physiol Renal Physiol. 2018 Oct 1;315(4):F927-F941.  IHC-P ;  Rat.   PubMed:29897288
Research Area	Cardiovascular  Neurobiology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	22kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Big endothelin-1: 16-110/212
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms - ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver. Endothelin 1 is expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. Function: Endothelins are endothelium-derived vasoconstrictor peptides. Subcellular Location: Secreted. Tissue Specificity: Expressed in lung, placental stem villi vessels and in cultured placental vascular smooth muscle cells. Similarity: Belongs to the endothelin/sarafotoxin family. SWISS: P05305 Gene ID: 1906 Database links: Entrez Gene: 1906 Human Entrez Gene: 13614 Mouse Entrez Gene: 24323 Rat Omim: 131240 Human SwissProt: P05305 Human SwissProt: P22387 Mouse SwissProt: P22388 Rat Unigene: 511899 Human Unigene: 713645 Human Unigene: 10918 Rat 内皮素(Endothelin，ET)是一种强的血管收缩剂，分为ET-1,ET-2,ET-3 三种，ET-1主要在endothelial cells中表达,而ET-2和ET-3则主要在神经系统中表达。ET具有广泛的生物学作用。此抗体是ET-1特异性抗体，与ET-2,ET-3无React Species。
Product Picture	Sample: Liver (Mouse) Lysate at 40 ug Liver (Rat) Lysate at 40 ug Primary: Anti-Big endothelin-1 (SL0188R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 22 kD Observed band size: 22 kD Sample: Lane 1: Testis (Mouse) Lysate at 40 ug Lane 2: Testis (Rat) Lysate at 40 ug Primary: Anti-Big endothelin-1 (SL0188R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 24 kD Observed band size: 26 kD Tissue/cell: Rat stomach tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti- Big endothelin-1 Polyclonal Antibody, Unconjugated(SL0118R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: Rat lung tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti- Big endothelin-1 Polyclonal Antibody, Unconjugated(SL0118R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (Rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Big endothelin-1) Polyclonal Antibody, Unconjugated (SL0188R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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