TEL: +86 571 56623320 EMAIL: [email protected]
Product Name Nociceptin receptor Chinese Name 孤菲肽受体/痛敏肽受体抗体 Alias H.sapiens mRNA for ORL1 receptor; Kappa type 3 opioid receptor; Kappa-type 3 opioid receptor; KOR 3; KOR-3; KOR3; LC132 receptor like; MGC34578; Nociceptin receptor; NOCIR; OOR; Opiate receptor like 1; Opiod receptor like 1; Oprl1; OPRX_HUMAN; ORL1; Orphanin FQ receptor. literatures Specific References (1) | SL0181R has been referenced in 1 publications.[IF=2.227] Xingrao Ke. et al. Adverse Maternal Environment Alters Oprl1 Variant Expression in Mouse Hippocampus. ANAT REC. 2022 Aug;: IHC ; Mouse.Research Area Cell biology Neurobiology Signal transduction Apoptosis The cell membrane受体 Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Dog, Pig, Cow, Horse, Guinea Pig, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 40kDa Cellular localization The cell membrane Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human Nociceptin receptor: 1-47/370 <Extracellular> Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail Nociceptin receptor Receptor for the neuropeptide nocipeptin/orphanin FQ. Has a potential role in modulating a number of brain functions, including instinctive behaviors and emotions. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase[subcellular location] integral membrane protein. Belongs to the G-protein coupled receptor 1 family.
Function:
Receptor for the neuropeptide nociceptin/orphanin FQ. Has a potential role in modulating a number of brain functions, including instinctive behaviors and emotions. The activity of this receptor is mediated by G proteins which inhibits adenylyl cyclase.
Subcellular Location:
Cell membrane; Multi-pass membrane protein.
Tissue Specificity:
In the brain, isoform KOR3 and isoform KOR3C are most abundant in hypothalamus and periaqueductal gray. Isoform KOR3A is highly expressed in cortex, striatum and brainstem. Isoform KOR3D is highly expressed in cerebellum, hypothalamus and brainstem.
Similarity:
Belongs to the G-protein coupled receptor 1 family.
SWISS:
P35377
Gene ID:
4987
Database links:Entrez Gene: 4987 Human
Entrez Gene: 18389 Mouse
Omim: 602548 Human
SwissProt: P41146 Human
SwissProt: P35377 Mouse
Unigene: 2859 Human
Unigene: 285075 Mouse
Unigene: 9762 Rat
Product Picture Sample:
Heart(Rat) lysate at 30ug;
Brain(Rat) lysate at 30ug;
Primary: Anti-Nociceptin receptor (SL0181R) at 1:200 dilution;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(SL0295G-HRP) at 1: 3000 dilution;
Predicted band size : 40kD
Observed band size : 40kD
Sample:
Heart (Mouse) Lysate at 40 ug
Primary: Anti- Nociceptin receptor (SL0181R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 52 kD
免疫组化(IHC)Immunohistochemistry Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Nociceptin receptor Polyclonal Antibody, Unconjugated(SL0181R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
免疫荧光Immunofluorescence 组织切片: Tissue/cell: human placenta tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Nociceptin receptor Polyclonal Antibody, Unconjugated(SL0181R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3conjugated (SL0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C.
Scan Wechat Qrcode