Product Name	PSD95
Chinese Name	突触后密度蛋白95抗体
Alias	Discs large homolog 4; Disks large homolog 4; DLG 4; DLG4; DLG4_HUMAN; FLJ97752; FLJ98574; Human post synaptic density protein 95; Human post-synaptic density protein 95; Post synaptic density protein 95; Postsynaptic density protein 95; PSD 95; PSD-95; PSD95; SAP 90; SAP-90; SAP90; Synapse associated protein 90; Synapse-associated protein 90; Tax interaction protein 15.
literatures	Specific References  (14)     |     SL0179R has been referenced in 14 publications. [IF=13.352] Tingting Ku. et al. Tebuconazole mediates cognitive impairment via the microbe-gut-brain axis (MGBA) in mice. ENVIRON INT. 2023 Feb;:107821  WB ;  Mouse.   PubMed:36827814 [IF=8.58] Ku, Tingting, et al. "NF-κB-regulated microRNA-574-5p underlies synaptic and cognitive impairment in response to atmospheric PM 2.5 aspiration." Particle and Fibre Toxicology 14.1 (2017): 34.  WB ;  Monkey, Mouse.   PubMed:28851397 [IF=7.26] Zhang, Chi, et al. "The potential use of H102 peptide-loaded dual-functional nanoparticles in the treatment of Alzheimer’s disease." Journal of Controlled Release (2014).  WB ;  Mouse.   PubMed:25102404 [IF=5.75] Zhifei Wang. et al. Human Cytomegalovirus Immediate Early Protein 2 Protein Causes Cognitive Disorder by Damaging Synaptic Plasticity in Human Cytomegalovirus-UL122-Tg Mice. Front Aging Neurosci. 2021; 13: 720582  IF,IHC ;  Mouse.   PubMed:34790111 [IF=5.714] Liu Yang. et al. Stress level of glucocorticoid exacerbates neuronal damage and Aβ production through activating NLRP1 inflammasome in primary cultured hippocampal neurons of APP-PS1 mice. INT IMMUNOPHARMACOL. 2022 Sep;110:108972  IF ;  Mouse.   PubMed:35777263 [IF=5.62] Zhao, Tianyun, et al. "Ketamine administered to pregnant rats in the second trimester causes long-lasting behavioral disorders in offspring." Neurobiology of Disease (2014).  WB ;  Rat.   PubMed:24780497 [IF=4.679] Dan Wu. et al. Protective effect of alpha-lipoic acid on bisphenol A-induced learning and memory impairment in developing mice: nNOS and keap1/Nrf2 pathway. Food Chem Toxicol. 2021 Aug;154:112307  IHC ;  Mouse.   PubMed:34058234 [IF=4.02] Wang, Chong, et al. "Changes in memory and synaptic plasticity induced in male rats after maternal exposure to Bisphenol A." Toxicology (2014).  WB ;  Rat.   PubMed:24820113 [IF=3.37] Dan Wu et al. Impairment of learning and memory induced by perinatal exposure to BPA is associated with ERα-mediated alterations of synaptic plasticity and PKC/ERK/CREB signaling pathway in offspring rats. Brain Res Bull. 2020 Aug;161:43-54.  IHC ;  Rat.   PubMed:32380187 [IF=2.997] Xinrong Pei. et al. The neuroprotective effects of alpha-lipoic acid on an experimental model of Alzheimer's disease in PC12 cells. 2021 Jun 16  WB ;  Rat.   PubMed:34133789 [IF=2.84] Li, Fei, et al. "MicroRNA-574 is involved in cognitive impairment in 5-month-old APP/PS1 mice through regulation of neuritin." Brain Research (2015).  WB ;  Mouse.   PubMed:26423933 [IF=2.173] Liu B et al. Effects of Autophagy on Synaptic-Plasticity-Related Protein Expression in the Hippocampus CA1 of a Rat Model of Vascular Dementia. Neurosci Lett. 2019 Jun 1;707:134312.  WB ;  Rat.   PubMed:31163225 [IF=1.89] Ku et al. Synergistic effects of particulate matter (PM2.5) and sulfur dioxide (SO2) on neurodegeneration via the microRNA-mediated regulation of tau phosphorylation. (2017) Toxicol.Res.(Camb). 6:7-16  WB ;  Mouse.   PubMed:30090473 [IF=1.28] Luo, Li, et al. "Niche astrocytes promote the survival, proliferation and neuronal differentiation of co‑transplanted neural stem cells following ischemic stroke in rats." Experimental and Therapeutic Medicine.  IF(IHC-P) ;  Rat.   PubMed:28352345
Research Area	Cell biology  immunology  Neurobiology  Cell type markers
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat, Monkey,  (predicted: Dog, Pig, Cow, Rabbit, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	80kDa
Cellular localization	The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human PSD95: 468-560/724
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family. It heteromultimerizes with another MAGUK protein, DLG2, and is recruited into NMDA receptor and potassium channel clusters. These two MAGUK proteins may interact at postsynaptic sites to form a multimeric scaffold for the clustering of receptors, ion channels, and associated signaling proteins. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] Function: Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ACCN3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B. Subunit: Interacts with ANKS1B, KLHL17 and PRR7. Interacts through its PDZ domains with NETO1. Interacts through its first two PDZ domains with GRIN2A, GRIN2B, GRIN2C, GRIN2D, ACCN3, certain splice forms of GRIN1, KCND2, CXADR and SYNGAP1. Interacts through its second PDZ domain with the PDZ domain of NOS1 or the C-terminus of CAPON. May interact with HTR2A. Interacts through its guanylate kinase-like domain with DLGAP1/GKAP, DLGAP2, DLGAP3, DLGAP4, MAP1A and BEGAIN. Interacts through its third PDZ domain with CRIPT (By similarity). Interacts through its first two PDZ domains with KCNA1, KCNA2, KCNA3, KCNA4 and ERBB4. Interacts through its first PDZ domain with GRIK2, KCNA4 and CRIPT. Interacts through its third PDZ domain with NLGN1, and probably with NLGN2 and NLGN3. Interacts through its guanylate kinase-like domain with KIF13B. Isoform 2 interacts through an L27 domain with HGS/HRS and the first L27 domain of CASK. Interacts with LRFN1, LRFN2 and LRFN4. Interacts with ANO2, ADAM22 and LGI1. Interacts with FRMPD4 (via C-terminus). Interacts (via PDZ1 and PDZ2 domains) with LRRC4; LRRC4B and SEMA4C. Interacts (via guanylate kinase-like domain) with SIPA1L1. Subcellular Location: Cell membrane; Peripheral membrane protein. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell junction, synapse. Cell junction, synapse, synaptosome. Note=High levels in postsynaptic density of neurons in the forebrain. Also in presynaptic region of inhibitory synapses formed by cerebellar basket cells on axon hillocks of Purkinje cells. Tissue Specificity: Brain. Post-translational modifications: Palmitoylation of isoform 1 is required for targeting to postsynaptic density. Similarity: Belongs to the MAGUK family. Contains 1 guanylate kinase-like domain. Contains 3 PDZ (DHR) domains. Contains 1 SH3 domain. SWISS: P78352 Gene ID: 1742 Database links: Entrez Gene: 1742 Human Entrez Gene: 13385 Mouse Entrez Gene: 29495 Rat Entrez Gene: 100137840 Cow Omim: 602887 Human SwissProt: P78352 Human SwissProt: Q62108 Mouse SwissProt: P31016 Rat Unigene: 463928 Human Unigene: 27256 Mouse Unigene: 9765 Rat 该蛋白质PSD95能夠帮助建立神经突触的架构，并且促成突触其他部分的成熟，例如加強目标細胞上的glutamate受体的聚集、增加树突棘（dendritic spines）的数量和大小以及增加神经传导物质glutamate (谷氨酸盐)释放的量。这种蛋白质与阿滋海默症有关。
Product Picture	Sample: Lane 1: Mouse Cerebrum tissue lysates Lane 2: Mouse Cerebellum tissue lysates Lane 3: Rat Cerebrum tissue lysates Lane 4: Rat Cerebellum tissue lysates Lane 5: Human U87MG cell lysates Lane 6: Human SH-SY5Y cell lysates Primary: Anti-PSD95 (SL0179R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 80 kDa Observed band size: 85 kDa Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PSD-95 Polyclonal Antibody, Unconjugated(SL0179R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-PSD-95 Polyclonal Antibody, Unconjugated(SL0179R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control (blue line): Mouse brain (blue). Primary Antibody (green line): Rabbit Anti-PSD95 antibody (SL0179R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 2% paraformaldehyde for 10 min at room temperature, and then stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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