Product Name	IRS1
Chinese Name	胰岛素受体底物-1抗体
Alias	IRS1_HUMAN; Insulin receptor substrate 1; IRS-1; IRS 1;
literatures	Specific References  (16)     |     SL0172R has been referenced in 16 publications. [IF=7.129] Yanwen Hou. et al. Prenatal PM2.5 exposure contributes to neuronal tau lesion in male offspring mice through mitochondrial dysfunction-mediated insulin resistance. ECOTOX ENVIRON SAFE. 2022 Nov;246:114151  WB ;  Mouse.   PubMed:36228359 [IF=7.097] Yilan Shen et al. VEGF-B antibody and interleukin-22 fusion protein ameliorates diabetic nephropathy through inhibiting lipid accumulation and inflammatory responses.  IHC ;  mouse.   PubMed:10.1016/j.apsb.2020.07.002 [IF=7.097] Yilan Shen et al. VEGF-B antibody and interleukin-22 fusion protein ameliorates diabetic nephropathy through inhibiting lipid accumulation and inflammatory responses.   PubMed:10.1016/j.apsb.2020.07.002 [IF=6.796] Tingting Ku. et al. Cardiac energy metabolism disorder mediated by energy substrate imbalance and mitochondrial damage upon tebuconazole exposure. J ENVIRON SCI-CHINA. 2022 Oct;:  WB ;  Mouse.   PubMed:10.1016/j.jes.2022.10.012 [IF=6.691] Wang, Hongyan. et al. Protection of pancreatic β-cell by phosphocreatine through mitochondrial improvement via the regulation of dual AKT/IRS-1/GSK-3β and STAT3/Cyp-D signaling pathways. 2021 Aug 28  WB ;  Rat.   PubMed:34455488 [IF=6.304] Xuemei Shen. et al. CircRILPL1 promotes muscle proliferation and differentiation via binding miR-145 to activate IGF1R/PI3K/AKT pathway. Cell Death Dis. 2021 Feb;12(2):1-14  WB ;  Bovine.   PubMed:33542215 [IF=6.208] Xuan Hu. et al. Effects of Chicken Serum Metabolite Treatment on the Blood Glucose Control and Inflammatory Response in Streptozotocin-Induced Type 2 Diabetes Mellitus Rats. INT J MOL SCI. 2023 Jan;24(1):523  IHC ;  Chicken.   PubMed:36613966 [IF=4.225] Chen Xuan. et al. Yiqi Jiedu Huayu Decoction Alleviates Renal Injury in Rats With Diabetic Nephropathy by Promoting Autophagy. Front Pharmacol. 2021; 12: 624404  WB ;  Rat.   PubMed:33912044 [IF=4.221] Huimin Wang. et al. Chronic exposure of bisphenol-A impairs cognitive function and disrupts hippocampal insulin signaling pathway in male mice. TOXICOLOGY. 2022 Apr;472:153192  WB ;  Mouse.   PubMed:35489422 [IF=3.69] Meng-fan Peng. et al. Effects of total flavonoids from Eucommia ulmoides Oliv. leaves on polycystic ovary syndrome with insulin resistance model rats induced by letrozole combined with a high-fat diet. J Ethnopharmacol. 2021 Jun;273:113947  WB ;  Rat.   PubMed:33617969 [IF=3.098] Zhang et al. Astragalus Polysaccharide Improves Insulin Sensitivity via AMPK Activation in 3T3-L1 Adipocytes. (2018) Molecules. 23  WB ;  Mouse.   PubMed:30347867 [IF=2.723] Cao, Hui. et al. 14-3-3β is essential for milk composition stimulated by Leu/IGF-1 via IGF1R signaling pathway in BMECs. IN VITRO CELL DEV-AN. 2022 Jun;:1-12  WB ;  Bovine.   PubMed:35648337 [IF=2.018] Yan X et al. Antidiabetic potential of green seaweed Enteromorpha prolifera flavonoids regulating insulin signaling pathway and gut microbiota in type 2 diabetic mice. (2018) J Food Sci.  WB ;  Mouse.   PubMed:30569533 [IF=1.832] Zhang T et al. Dietary Sea Buckthorn Pomace Induces Beige Adipocyte Formation in Inguinal White Adipose Tissue in Lambs. Animals (Basel). 2019 Apr 24;9(4). pii: E193.  WB ;  ram lambs.   PubMed:31022943 [IF=1.81] Dong, Peiyue, et al. "MiR-15a/b promote adipogenesis in porcine pre-adipocyte via repressing FoxO1." Acta Biochimica et Biophysica Sinica (2014): gmu043.  WB ;  Pig.   PubMed:24862853 [IF=1.513] Ting Zhang. et al. Sea buckthorn ( Hippophae rhamnoides L.) oil enhances proliferation, adipocytes differentiation and insulin sensitivity in 3T3-L1 cells. Food Sci Biotechnol. 2020 Nov;29(11):1511-1518  WB ;  Mouse.   PubMed:33088600
Research Area	Cell biology  Neurobiology  Signal transduction  Apoptosis  Diabetes  Endocrinopathy
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat, Pig,  (predicted: Chicken, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	137kDa
Cellular localization	The nucleus cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from rat IRS-1: 1101-1200/1242
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Insulin receptor substrates (IRS) are responsible for several insulin related activities, such as glucose homeostasis, cell growth, cell transformation, apoptosis and insulin signal transduction. Serine/threonine phosphorylation of IRS1 has been demonstrated to be a negative regulator of insulin signaling and is responsible for its degradation, although IRS1 degradation pathways are not well understood. IRS1 has also been shown to be constitutively activated in cancers such as breast cancer, Wilm's tumors, and adrenal cortical carcinomas, thus making IRS1 phosphorylation and subsequent degradation an attractive therapeutic target. To date there have been four subtypes identified: IRS1, 2, 3 and 4, with IRS1 being widely expressed. Function: May mediate the control of various cellular processes by insulin. When phosphorylated by the insulin receptor binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2. Activates phosphatidylinositol 3-kinase when bound to the regulatory p85 subunit. Subunit: Interacts with UBTF and PIK3CA. Interacts (via phosphorylated YXXM motifs) with PIK3R1. Interacts with ROCK1 and FER. Interacts (via PH domain) with PHIP. Interacts with GRB2. Interacts with SOCS7. Interacts (via IRS-type PTB domain) with IGF1R and INSR (via the tyrosine-phosphorylated NPXY motif). Interacts with ALK. Subcellular Location: IRS1 is predominantly found in the cytoplasm. Nuclear localization may occur in some cell types and under specific stimuli. Post-translational modifications: Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the insulin receptor. Phosphorylation of Tyr-896 is required for GRB2-binding. DISEASE: Polymorphisms in IRS1 may be involved in the etiology of non-insulin-dependent diabetes mellitus (NIDDM) Similarity: Contains 1 IRS-type PTB domain. Contains 1 PH domain. SWISS: P35568 Gene ID: 3667 Database links: Entrez Gene: 3667 Human Entrez Gene: 16367 Mouse Entrez Gene: 25467 Rat SwissProt: P35568 Human SwissProt: P35569 Mouse SwissProt: P35570 Rat 信号传导（Signaling Intermediates） 胰岛素受体底物-1是细胞中分子量为165-185kDa的磷蛋白，胰岛素受体的内源性底物。经胰岛素刺激后其酪氨酸残基被磷酸化。
Product Picture	Paraformaldehyde-fixed, paraffin embedded (rat pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IRS1) Polyclonal Antibody, Unconjugated (SL0172R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IRS1) Polyclonal Antibody, Unconjugated (SL0172R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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