Product Name	eNOS
Chinese Name	一氧化氮合成酶-3（内皮型）抗体
Alias	NOS-3; cNOS; Constitutive NOS; EC NOS; ecNOS; Endothelial nitric oxidase synthase; Endothelial nitric oxide synthase; Endothelial nitric oxide synthase 3; Endothelial NOS; Nitric oxide synthase 3 (endothelial cell); Nitric oxide synthase 3; Nitric oxide synthase 3 endothelial cell; Nitric oxide synthase endothelial; nitric oxide synthase, endothelial; NOS 3; NOS III; NOS type III; NOS3; NOSIII; NOS3_HUMAN.
literatures	Specific References  (17)     |     SL0163R has been referenced in 17 publications. [IF=5.51] Nour, Sayed, et al. "Intrapulmonary shear stress enhancement: A new therapeutic approach in acute myocardial ischemia." International Journal of Cardiology (2013).  Pig.   PubMed:23932859 [IF=4.12] Yang et al. In situ eNOS/NO up-regulation-a simple and effective therapeutic strategy for diabetic skin ulcer. (2016) Sci.Rep. 6:30326  IHC ;  Rat.   PubMed:27453476 [IF=3.457] Gao Y et al. Ginsenoside Re inhibits vascular neointimal hyperplasia in balloon-injured carotid arteries through activating the eNOS/NO/cGMP pathway in rats Y Gao, CY Gao, P Zhu, SF Xu, YM Luo, J Deng… - Biomedicine & …, 2018Biomed Pharmacother. 2018 Oct;106:1091-1097.  IHC-P ;  Rat.   PubMed:30119175 [IF=3.337] Liang Huang. et al. Ginsenoside Rg1 promoted the wound healing in diabetic foot ulcers via miR-489–3p/Sirt1 axis. J Pharmacol Sci. 2021 Aug;:  IHC ;  Rat.   PubMed:34507636 [IF=3.14] Li, Hongyan, et al. "Oxidative stress, endothelial dysfunction and inflammatory response in rat heart to NO< sub> 2 inhalation exposure." Chemosphere 82.11 (2011): 1589-1596.  WB ;  Rat.   PubMed:21168897 [IF=3.05] Ji, Chenyang, et al. "Construction of tissue‐engineered corpus cavernosum with muscle‐derived stem cells and transplantation in vivo." BJU international 107.10 (2011): 1638-1646.  Rabbit.   PubMed:20868390 [IF=2.948] Gokhan Ozge. et al. Salubrinal Ameliorates Inflammation and Neovascularization via the Caspase 3/Enos Signaling in an Alkaline-Induced Rat Corneal Neovascularization Model. MED LITH. 2023 Feb;59(2):323  IHC ;  Rat.   PubMed:36837524 [IF=2.768] Chen L et al. Compressive force-induced autophagy in periodontal ligament cells downregulatesosteoclastogenesis during tooth movement. J Periodontol. 2019 May 11.  WB ;  Mouse&Human.   PubMed:31077358 [IF=2.59] Wu, Chun Shan, et al. "Bubble CPAP Support after Discontinuation of Mechanical Ventilation Protects Rat Lungs with Ventilator-Induced Lung Injury." Respiration (2016).  other ;   PubMed:26800273 [IF=2.59] Sun, Tao, et al. "Antihypertensive effect of formononetin through regulating the expressions of eNOS, 5-HT< sub> 2A/1B receptors and α< sub> 1-adrenoceptors in spontaneously hypertensive rat arteries." European Journal of Pharmacology (2013).  Rat.   PubMed:23123056 [IF=1.813] Fan Q et al. Phenylethanol Glycosides Protect Myocardial Hypertrophy Induced by Abdominal Aortic Constriction via ECE-1 Demethylation Inhibition and PI3K/PKB/eNOS Pathway Enhancement. Evid Based Complement Alternat Med. 2020 Jun 9;2020:2957094.  WB ;  Rat.   PubMed:32595726 [IF=1.732] Li B et al. Oxidative stress mediates renal endothelial cell damage in trichloroethylene-sensitized mice. J Toxicol Sci. 2019;44(5):317-326.  IHC-P ;  Mouse.   PubMed:31068537 [IF=1.48] Wen, Chen-Ting, Tao He, and Yi-Qiao Xing. "Erythropoietin promotes retinal angiogenesis in a mouse model." Molecular medicine reports 10.6 (2014): 2979-2984.  WB ;  Mouse.   PubMed:25269825 [IF=1.38] Hu, Zhibin, et al. "Comparative analysis of preservation method and intermittent perfusion volume on the expression of endothelial and inflammatory markers by coronal artery and myocardium in porcine donor hearts." ASAIO journal (American Society for Artificial Internal Organs: 1992) (2014).  IHC-P ;  Pig.   PubMed:25232770 [IF=0.939] Prayitnaningsih et al. Neuropathy optic glaucomatosa induced by systemic hypertension through activation endothelin-1 signaling pathway in central retinal artery in rats. (2016) Int.J.Ophthalmol. 9:1568-1577  IF(IHC-P) ;  Rat.   PubMed:27990358 [IF=0.882] Ali Tuğrul Akin. et al. Therapeutic effects of thymoquinone in doxorubicin-induced hepatotoxicity via oxidative stress, inflammation and apoptosis. 2021 Sep 08  IHC ;  rat.   PubMed:34494664 [IF=0] Jin et al. Icariin, a phosphodiesterase-5 inhibitor, improves learning and memory in APP/PS1 transgenic mice by stimulation of NO/cGMP signalling. (2014) Int.J.Neuropsychopharmacol. 17:871-81  WB ;  Mouse.   PubMed:24513083
Research Area	Neurobiology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat,  (predicted: Mouse, Dog, Pig, Cow, Sheep, Guinea Pig, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	133kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from mouse eNOS: 1105-1202/1203
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Nitric oxide synthase NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti tumor and anti pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); inducible NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). eNOS is a calcium/calmodulin dependent enzyme which undergoes several post translational modifications, including acylation with myristate and palmitate, and phosphorylation on numerous residues. As with the other members of the NOS family, eNOS derives the diffusible multifunctional second messenger NO from L arginine through a series of reactions in which L citrulline is an intermediate. eNOS plays an important role in controlling vascular tone, platelet aggregation, and cardiac myocyte function. Function: Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets. Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1. Subunit: Homodimer. Interacts with NOSIP and NOSTRIN. Subcellular Location: Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity. Tissue Specificity: Platelets, placenta, liver and kidney. Post-translational modifications: Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity. Phosphorylation of Ser-114 by CDK5 reduces activity. Similarity: Belongs to the NOS family. Contains 1 FAD-binding FR-type domain. Contains 1 flavodoxin-like domain. SWISS: P70313 Gene ID: 18127 Database links: Entrez Gene: 287024 Cow Entrez Gene: 4846 Human Entrez Gene: 18127 Mouse Entrez Gene: 24600 Rat Omim: 163729 Human SwissProt: P29473 Cow SwissProt: P29474 Human SwissProt: P70313 Mouse SwissProt: Q62600 Rat Unigene: 647092 Human Unigene: 258415 Mouse Unigene: 44265 Rat Synthesis and Degradation（Synthesis and Degradation) 催化生物体内一氧化氮（NO）生成的酶。分神经型一氧化氮合成的酶（nNOS or NOS-1）、诱导型一氧化氮合成的酶（iNOS or NOS-2）、内皮型一氧化氮合成的酶（eNOS or NOS-3）。此抗体识别分子量为135kDa的内皮型一氧化氮合成的酶。
Product Picture	Tissue/cell: rat liver tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NOS-3/eNOS Polyclonal Antibody, Unconjugated(SL0163R) 1:100, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

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