Product Name	Neurotrophin 3
Chinese Name	神经营养因子-3前蛋白抗体
Alias	HDNF; MGC129711; Nerve growth factor 2; Neurotrophic factor; Neurotrophin 3 precursor; Neurotrophin-3; Neurotrophin3; NGF 2; NGF-2; NGF2; NT 3; NT-3; NT3; NTF 3; NTF3; NTF3_HUMAN.
literatures	Specific References  (1)     |     SL0160R has been referenced in 1 publications. [IF=5.01] Guo, Hongrui, et al. "Dietary NiCl2 causes cell cycle arrest in the broiler's kidney." Oncotarget. (2015) 6.34:35964-77.  other ;  Rat.   PubMed:26440151
Research Area	immunology  Neurobiology
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Cow, )
Applications	ELISA=1:5000-10000 IHC-P=1:200-500 IHC-F=1:200-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	28kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human NT-3: 19-100/258
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse. [provided by RefSeq, Jul 2008]. Function: Seems to promote the survival of visceral and proprioceptive sensory neurons. Subcellular Location: Secreted. Tissue Specificity: Brain and peripheral tissues. Similarity: Belongs to the NGF-beta family. SWISS: P20783 Gene ID: 4908 Database links: Entrez Gene: 4908 Human Entrez Gene: 18205 Mouse Entrez Gene: 81737 Rat Omim: 162660 Human SwissProt: P20783 Human SwissProt: P20181 Mouse SwissProt: P18280 Rat Unigene: 99171 Human Unigene: 267570 Mouse Unigene: 9715 Rat Neurobiology相关蛋白（Neurobiology） 一种内源型神经递质，分子量为28kDa的碱性多肽，属神经营养蛋白家族。与NGF和BDNF具有序列同源性，为培养的神经嵴细胞的促细胞分裂剂。此抗体与NGF、BDNF无React Species。
Product Picture	Paraformaldehyde-fixed, paraffin embedded (rat skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Neurotrophin 3 ) Polyclonal Antibody, Unconjugated (SL0160R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Neurotrophin 3 ) Polyclonal Antibody, Unconjugated (SL0160R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Neurotrophin 3 ) Polyclonal Antibody, Unconjugated (SL0160R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse skin); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Neurotrophin 3 ) Polyclonal Antibody, Unconjugated (SL0160R) at 1:1000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NTF3 Polyclonal Antibody, Unconjugated(SL0160R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-NTF3 Polyclonal Antibody, Unconjugated(SL0160R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Neurotrophin 3) Polyclonal Antibody, Unconjugated (SL0160R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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