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Product Name HSP90 beta Chinese Name 热休克蛋白90β/HSP90 β 抗体 Alias HSP90 beta; D6S182; FLJ26984; Heat shock 90kD protein 1, beta; Heat shock protein 90kDa alpha (cytosolic) class B member 1; Heat shock protein beta; Heat shock protein HSP 90 beta; HSP 84; HSP84; HSP 90; HSP-90 BETA; HSP90B; HSPC2; HSPCB; HS90B_HUMAN; Heat shock protein HSP 90-beta; Heat shock 84 kDa. literatures Specific References (4) | SL0135R has been referenced in 4 publications.[IF=4.258] Mori et al. MUC1 protein induces urokinase-type plasminogen activator (uPA) by forming a complex with NF-κB p65 transcription factor and binding to the uPA promoter, leading to enhanced invasiveness of cancer cells. (2014) J.Biol.Che. 289:35193-204 WB ; Human.[IF=3.24] Ding, Zhujin, et al. "Expression of heat shock protein 90 genes during early development and infection in< i> Megalobrama amblycephala and evidence for adaptive evolution in teleost." Developmental & Comparative Immunology (2013). WB ;[IF=2.133] Cetintas, Vildan Bozok, et al. "Effects of flavopiridol on critical regulation pathways of CD133high/CD44high lung cancer stem cells." Medicine 95.43 (2016): e5150. IF(ICC) ; Mouse.[IF=0.46] Aktug, H., et al. "Investigation of the Cell Stabilization and the Epithelial to Mesenchymal Transition Effect of Flavopiridol in Mouse Lung Squamous Cell Carcinoma." Chemotherapy: Open Access 2015 (2015). IF(ICC) ; Mouse.Research Area Tumour Cell biology immunology Signal transduction Apoptosis Immunogen Species Rabbit Clonality Polyclonal React Species Human, Mouse, Rat, (predicted: Chicken, Dog, Pig, Cow, ) Applications WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100 IF=1:100-500 (Paraffin sections need antigen repair)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Theoretical molecular weight 84kDa Cellular localization cytoplasmic Form Liquid Concentration 1mg/ml immunogen KLH conjugated synthetic peptide derived from human HSP90 beta: 635-724/724 Lsotype IgG Purification affinity purified by Protein A Buffer Solution 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. Attention This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. PubMed PubMed Product Detail This gene encodes a member of the heat shock protein 90 family; these proteins are involved in signal transduction, protein folding and degradation and morphological evolution. This gene encodes the constitutive form of the cytosolic 90 kDa heat-shock protein and is thought to play a role in gastric apoptosis and inflammation. Alternative splicing results in multiple transcript variants. Pseudogenes have been identified on multiple chromosomes. [provided by RefSeq, Dec 2012].
Function:
Molecular chaperone that promotes the maturation, structural maintenance and proper regulation of specific target proteins involved for instance in cell cycle control and signal transduction. Undergoes a functional cycle that is linked to its ATPase activity. This cycle probably induces conformational changes in the client proteins, thereby causing their activation. Interacts dynamically with various co-chaperones that modulate its substrate recognition, ATPase cycle and chaperone function.
Subunit:
Homodimer. Interacts with p53/TP53. Forms a complex with CDK6 and Hsp90/HSP90AB1. Interacts with UNC45A. Binding to UNC45A involves 2 UNC45A monomers per HSP90AB1 dimer. Interacts with CHORDC1 and DNAJC7. Interacts with FKBP4.
Subcellular Location:
Cytoplasm. Melanosome. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Post-translational modifications:
Ubiquitinated in the presence of STUB1-UBE2D1 complex (in vitro).
ISGylated.
S-nitrosylated; negatively regulates the ATPase activity (Probable).
Similarity:
Belongs to the heat shock protein 90 family.
SWISS:
P08238
Gene ID:
3326
Database links:Entrez Gene: 3326 Human
Entrez Gene: 15516 Mouse
Omim: 140572 Human
SwissProt: P08238 Human
SwissProt: P11499 Mouse
Unigene: 509736 Human
Unigene: 2180 Mouse
Unigene: 98667 Rat
HSP是细胞受应激原刺激后诱导产生的一组应激蛋白,与Tumour发生、增殖及分化有关,HSP90β能够抑制Apoptosis与分化。Product Picture Sample:
HepG2 Cell Lysate at 40 ug
Cerebrum (Mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti- HSP90 beta (SL0135R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 84kD
Observed band size: 90kD
Sample:
Lane 1: A431 (Human) Cell Lysate at 30 ug
Lane 2: Hela (Human) Cell Lysate at 30 ug
Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 4: K562 (Human) Cell Lysate at 30 ug
Lane 5: HepG2 (Human) Cell Lysate at 30 ug
Lane 6: U2os (Human) Cell Lysate at 30 ug
Lane 7: Testis (Mouse) Lysate at 40 ug
Primary: Anti-HSP90 beta (SL0135R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 84-90 kD
Observed band size: 84 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (HSP90 beta) Polyclonal Antibody, Unconjugated (SL0135R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (HSP90 beta) polyclonal Antibody, Unconjugated (SL0135R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.U2OS cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (HSP90 beta) polyclonal Antibody, Unconjugated (SL0135R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
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