Product Name	TGF beta 1
Chinese Name	转化生长因子β1/TGF β1/TGF-β1抗体
Alias	CED; DPD1; TGF beta 1; TGF beta; TGF beta 1 protein; TGF-beta 1 protein; TGF-beta-1; TGF-beta-5; TGF-beta1; TGFB; Tgfb-1; tgfb1; TGFB1_HUMAN; TGFbeta; TGFbeta1; Transforming Growth Factor b1; Transforming Growth Factor beta 1; Transforming growth factor beta 1a; transforming growth factor beta-1; transforming growth factor, beta 1.
literatures	Specific References  (94)     |     SL0086R has been referenced in 94 publications. [IF=16.744] Lubin Zhou. et al. A self-pumping dressing with in situ modification of non-woven fabric for promoting diabetic wound healing. CHEM ENG J. 2022 Dec;:141108  IHC ;  Rat.   PubMed:10.1016/j.cej.2022.141108 [IF=10.508] Xiaonan Li. et al. Facile One-Pot Synthesis of Meteor Hammer-like Au-MnOx Nanozymes with Spiky Surface for NIR-II Light-Enhanced Bacterial Elimination. CHEM MATER. 2022;XXXX(XXX):XXX-XXX  IF ;  Rat.   PubMed:10.1021/acs.chemmater.2c01775 [IF=9.038] Bing Han. et al. Pulmonary inflammatory and fibrogenic response induced by graphitized multi-walled carbon nanotube involved in cGAS-STING signaling pathway. J Hazard Mater. 2021 May;:125984  WB ;  Mouse.   PubMed:34020360 [IF=8.786] Xiaoyu Guo. et al. Effects of Boric Acid Gel on Vaginal Candida albicans Infections and the Local Immune System in Mice. FRONT IMMUNOL. 2022; 13: 950215  IHC ;  Mouse.   PubMed:35958550 [IF=7.901] Lin S et al. Strategy for hypertrophic scar therapy: Improved delivery of triamcinolone acetonide using mechanically robust tip-concentrated dissolving microneedle array. J Control Release. 2019 May 27;306:69-82.  WB ;  Rabbit.   PubMed:31145948 [IF=7.577] Wei C et al. Nanocolloids in drinking water increase the risk of obesity in mice by modulating gut microbesEnviron Int.2021 Jan;146:106302.  IHC ;  Mouse.   PubMed:33395945 [IF=7.419] Xiaofang Tao. et al. PRDX6 inhibits hepatic stellate cells activation and fibrosis via promoting MANF secretion. BIOMED PHARMACOTHER. 2022 Dec;156:113931  WB ;  Rat.   PubMed:10.1016/j.biopha.2022.113931 [IF=7.39] Ganguly, Rituparna, et al. "Anti-atherogenic Effect of Trivalent Chromium-loaded CPMV Nanoparticles in Human Aortic Smooth Muscle Cells under Hyperglycemic Conditions in vitro." Nanoscale (2016).  WB ;  Human.   PubMed:26935414 [IF=6.51] Qingliang Yang. et al. 3D-printed morphology-customized microneedles: understanding the correlation between their morphologies and the received qualities. INT J PHARMACEUT. 2023 Mar;:122873  IHC,WB ;  Rabbit.   PubMed:36958610 [IF=6.291] Si-Cheng Zhao. et al. Nickel sulfate exposure induces ovarian inflammation and fibrosis and decreases oocyte quality in mice. Ecotox Environ Safe. 2021 Nov;224:112634  WB ;  Mouse.   PubMed:34392153 [IF=6.291] Wei Cui. et al. Cadmium exposure activates the PI3K/AKT signaling pathway through miRNA-21, induces an increase in M1 polarization of macrophages, and leads to fibrosis of pig liver tissue. Ecotox Environ Safe. 2021 Dec;228:113015  WB ;  Pig.   PubMed:34823215 [IF=6.208] Albert chun-shuo Huang. et al. NF-κB Decoy ODN-Loaded Poly(Lactic-co-glycolic Acid) Nanospheres Inhibit Alveolar Ridge Resorption. INT J MOL SCI. 2023 Jan;24(4):3699  IHC ;  Rat.   PubMed:36835111 [IF=6.1] Tuling Li. et al. Inhibitory effect of miR-138-5p on choroidal fibrosis in lens-induced myopia guinea pigs via suppressing the HIF-1α signaling pathway. BIOCHEM PHARMACOL. 2023 May;211:115517  WB ;  Guinea pig.   PubMed:36966935 [IF=6.048] Qingqing Liu. et al. Probiotics alleviate autoimmune hepatitis in mice through modulation of gut microbiota and intestinal permeability. J Nutr Biochem. 2021 Sep;:108863  IHC ;  mosue.   PubMed:34517094 [IF=5.923] Kun-Ling Lin. et al. Low-Intensity Extracorporeal Shock Wave Therapy Promotes Bladder Regeneration and Improves Overactive Bladder Induced by Ovarian Hormone Deficiency from Rat Animal Model to Human Clinical Trial. Int J Mol Sci. 2021 Jan;22(17):9296  WB ;  Rat.   PubMed:34502202 [IF=5.714] Xue Wu. et al. CD73 aggravates alcohol-related liver fibrosis by promoting autophagy mediated activation of hepatic stellate cells through AMPK/AKT/mTOR signaling pathway. INT IMMUNOPHARMACOL. 2022 Dec;113:109229  WB ;  Mouse, Rat.   PubMed:36330907 [IF=5.682] Xiaolan You. et al. Dihydroartemisinin attenuates pulmonary inflammation and fibrosis in rats by suppressing JAK2/STAT3 signaling. Aging-Us. 2022 Feb 15; 14(3): 1110–1127  IHC,WB ;  Rat.   PubMed:35120332 [IF=5.682] Xiaolan You. et al. Fibroblastic galectin-1-fostered invasion and metastasis are mediated by TGF-β1-induced epithelial-mesenchymal transition in gastric cancer. Aging-Us. 2021 Jul 31; 13(14): 18464–18481  WB,IHC ;  Mouse.   PubMed:34260413 [IF=5.5] Zhang et al. Cardiac Contractility Modulation Attenuate Myocardial Fibrosis by Inhibiting TGF-β1/Smad3 Signaling Pathway in a Rabbit Model of Chronic Heart Failure. (2016) Cell.Physiol.Biochem. 39:294-306  WB ;  Rabbit.   PubMed:27344462 [IF=5.396] Yan Zhang. et al. Polysaccharide from Ganoderma lucidum Alleviates Congnitive Impairment in a Mice Model of Chronic Cerebral Hypoperfusion by Regulating CD4+CD25+Foxp3+ regulatory T cells. Food Funct. 2022 Jan;:  WB ;  Mouse.   PubMed:35088782 [IF=5.29] Shang, Peijin, et al. "Acetyl-11-Keto-β-Boswellic Acid Attenuates Prooxidant and Profibrotic Mechanisms Involving Transforming Growth Factor-β1, and Improves Vascular Remodeling in Spontaneously Hypertensive Rats." Scientific Reports 6 (2016): 39809.  IHC-P ;  Rat.   PubMed:28009003 [IF=5.279] Yan Zhang. et al. Ganoderic Acid A To Alleviate Neuroinflammation of Alzheimer’s Disease in Mice by Regulating the Imbalance of the Th17/Tregs Axis. J Agr Food Chem. 2021;69(47):14204–14214  WB ;  Mouse.   PubMed:34798773 [IF=5.241] Wang, Lingfang. et al. miR-2337 induces TGF-β1 production in granulosa cells by acting as an endogenous small activating RNA. Cell Death Discov. 2021 Sep;7(1):1-9  WB ;   Porcine.   PubMed:34537818 [IF=5.223] Li-You Chen. et al. Luteolin improves nephropathy in hyperglycemic rats through anti-oxidant, anti-inflammatory, and anti-apoptotic mechanisms. J FUNCT FOODS. 2023 Mar;102:105461  WB ;  Rat.   PubMed:10.1016/j.jff.2023.105461 [IF=5.157] Yu Zhang. et al. Follistatin-like 1 (FSTL1) interacts with Wnt ligands and Frizzled receptors to enhance Wnt/β-catenin signaling in obstructed kidneys in vivo. J BIOL CHEM. 2022 May;:102010  WB ;  Human.   PubMed:35525270 [IF=4.996] Zhou Zihe. et al. External beam radiotherapy inhibits stent related granulation hyperplasia in rabbit trachea. SCI REP-UK. 2023 May;13(1):1-8  IHC ;  Rabbit.   PubMed:37137968 [IF=4.679] Le G et al. Ochratoxin A induces glomerular injury through activating the ERK/NF-κB signaling pathway. Food Chem Toxicol. 2020 Sep;143:111516.  WB&ICF ;  Human.   PubMed:32615238 [IF=4.679] Le G et al. Ochratoxin A Induces Glomerular Injury Through Activating the ERK/NF-κB Signaling Pathway. Food Chem Toxicol. 2020 Jun 29;111516.  WB,IF ;  human.   PubMed:32615238 [IF=4.679] Guannan Leet al. Ochratoxin A induces glomerular injury through activating the ERK/NF-κB signaling pathway. Food Chem Toxicol . 2020 Sep;143:111516.  WB ;  Human.   PubMed:32615238 [IF=4.545] Yi Zhang. et al. Nanoparticle conjugation of ginsenoside Rb3 inhibits myocardial fibrosis by regulating PPARα pathway. Biomed Pharmacother. 2021 Jul;139:111630  WB ;  Rat.   PubMed:33945912
Research Area	Tumour  Cardiovascular  Signal transduction  Stem cells  Growth factors and hormones  transcriptional regulatory factor  cell factor  Extracellular matrix
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, Cow, Rabbit, Sheep, Guinea Pig, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg /Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	12.8/44kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human TGF-Beta 1: 351-390/390
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a secreted ligand of the TGF-beta (transforming growth factor-beta) superfamily of proteins. Ligands of this family bind various TGF-beta receptors leading to recruitment and activation of SMAD family transcription factors that regulate gene expression. The encoded preproprotein is proteolytically processed to generate a latency-associated peptide (LAP) and a mature peptide, and is found in either a latent form composed of a mature peptide homodimer, a LAP homodimer, and a latent TGF-beta binding protein, or in an active form consisting solely of the mature peptide homodimer. The mature peptide may also form heterodimers with other TGFB family members. This encoded protein regulates cell proliferation, differentiation and growth, and can modulate expression and activation of other growth factors including interferon gamma and tumor necrosis factor alpha. This gene is frequently upregulated in tumor cells, and mutations in this gene result in Camurati-Engelmann disease. [provided by RefSeq, Aug 2016] Function: Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Subunit: Homodimer; disulfide-linked, or heterodimer with TGFB2. Secreted and stored as a biologically inactive form in the extracellular matrix in a 290 kDa complex (large latent TGF-beta1 complex) containing the TGFB1 homodimer, the latency-associated peptide (LAP), and the latent TGFB1 binding protein-1 (LTBP1). The complex without LTBP1 is known as the'small latent TGF-beta1 complex'. Dissociation of the TGFB1 from LAP is required for growth factor activation and biological activity. Release of the large latent TGF-beta1 complex from the extracellular matrix is carried out by the matrix metalloproteinase MMP3 (By similarity). May interact with THSD4; this interaction may lead to sequestration by FBN1 microfibril assembly and attenuation of TGFB signaling. Interacts with the serine proteases, HTRA1 and HTRA3: the interaction with either inhibits TGFB1-mediated signaling. The HTRA protease activity is required for this inhibition (By similarity). Interacts with CD109, DPT and ASPN. Subcellular Location: Secreted, extracellular space, extracellular matrix. Tissue Specificity: Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage. Post-translational modifications: Glycosylated. The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. DISEASE: Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision. Similarity: Belongs to the TGF-beta family. SWISS: P01137 Gene ID: 7040 Database links: Entrez Gene: 7040 Human Entrez Gene: 21803 Mouse Entrez Gene: 59086 Rat Omim: 190180 Human SwissProt: P01137 Human SwissProt: P04202 Mouse SwissProt: P17246 Rat Unigene: 645227 Human Growth factors and hormones（ Growth Factor and Hormones） TGF是一种多效生长因子，对epithelial cells增值有潜在抑制作用，可抑制Tumour生长用于许多恶性Tumour如:胃癌、肺癌、膀胱癌、肾癌、前列腺癌、结肠癌等多种恶性Tumour的研究。 TGFβ超级家族由为数众多的生长的分化因子组成，包括转移生长因子β1，2和3(TGF β1，TGF β2，TGF β3)；胎盘生长因子(PTGF-β)；生长/分化因子(GDFs)；缪氏抑制物(MIS)；骨形态形成蛋白(BMPs)；交织细胞元神经生长因子(GDNF)；抑制素和活化素(α, β-A,和β-C)，Lefty和Nodal。 TGF超级家族成员参与胚胎发育和成体组织体内平衡。TGF-β1抗体与TGF-β2和TGF-β3没有React Species。与豚鼠有大部分交叉。
Product Picture	Sample: Embryo (Mouse) Lysate at 40 ug Cerebrum (Rat) Lysate at 40 ug Primary: Anti-TGF beta 1 (SL0086R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12.8/44 kD Observed band size: 59 kD Sample: Ovary (Mouse) Lysate at 40 ug Primary: Anti-TGF beta 1 (SL0086R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12.8/44 kD Observed band size: 59 kD Sample: Liver (Mouse) Lysate at 40 ug Liver (Rat) Lysate at 40 ug Primary: Anti- TGF beta 1 (SL0086R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12.8/44 kD Observed band size: 55 kD Sample:TGF beta1 recombinant protein (human) Lysate at 100ng Primary: Anti- TGF beta1 (SL0086R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 12.8/44 kD Observed band size: 12.8 kD Sample: Lane 1: Stomach (Mouse) Lysate at 40 ug Lane 2: Liver (Mouse) Lysate at 40 ug Lane 3: Placenta (Mouse) Lysate at 40 ug Lane 4: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 5: Liver (Rat) Lysate at 40 ug Primary: Anti-TGF beta 1 (SL0086R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 50/25/12.5 kD Observed band size: 50 kD Paraformaldehyde-fixed, paraffin embedded (Rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TGF beta 1) Polyclonal Antibody, Unconjugated (SL0086R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining. Blank control:MG63. Primary Antibody (green line): Rabbit Anti-TGF beta 1 antibody (SL0086R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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