Product Name	Caspase 8
Chinese Name	半胱氨酸蛋白酶8抗体
Alias	Caspase-8 p10; Caspase-8 subunit p10; ALPS2B; Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein; Apoptosis related cysteine peptidase; Apoptotic cysteine protease; Apoptotic protease Mch 5; Apoptotic protease Mch-5; Apoptotic protease Mch5; CAP 4; CAP4; CASP 8; CASP-8; Caspase-8; Caspase8; CASP8; CASP8_HUMAN; Caspase8; Caspase 8 apoptosis related cysteine peptidase; Caspase8; CED 3; FADD homologous ICE/CED 3 like protease; FADD Homologous ICE/CED3 Like Protease; FADD Like ICE antibody FADD-homologous ICE/CED-3-like protease; FADD-like ICE; FLICE; FLJ17672; ICE like apoptotic protease 5; ICE-like apoptotic protease 5; MACH alpha 1/2/3 protein; MACH antibody MACH beta 1/2/3/4 protein antibody MCH 5; MCH5.
literatures	Specific References  (23)     |     SL0052R has been referenced in 23 publications. [IF=2.914]   WB ;  Pig.   PubMed:32649964 [IF=6.575] Anvita Gupta. et al. Androgen Receptor Activation Induces Senescence in Thyroid Cancer Cells. CANCERS. 2023 Jan;15(8):2198  IF ;  Human.   PubMed:37190127 [IF=5.23] Zhao, Yong, et al. "Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways." Scientific Reports 6 (2016): 37884.  WB ;  Pig.   PubMed:27883089 [IF=4.2] Zhang, Weidong, et al. "Decrease in male mouse fertility by hydrogen sulfide and/or ammonia can Be inheritable." Chemosphere (2017).  IHC-P ;  Mouse.   PubMed:29202267 [IF=4.171] Jian Wang. et al. Baicalin ameliorates Mycoplasma gallisepticum-induced inflammatory injury in chicken lung through regulating intestinal microbiota and phenylalanine metabolism. 2021 Mar 30  WB ;  Chicken.   PubMed:10.1039/D1FO00055A [IF=3.95] Wang, Gang, et al. "Inhibition of hydrogen sulfide synthesis provides protection for severe acute pancreatitis rats via apoptosis pathway." Apoptosis (2013): 1-15.  IHC-P ;  Rat.   PubMed:23054084 [IF=3.85] Wang, Yandi, et al. "Regulation of steroid hormones and energy status with cysteamine and its effect on spermatogenesis." Toxicology and Applied Pharmacology (2016).  IHC-P ;  Sheep.   PubMed:27815134 [IF=3.775] Wang X et al. Dibutyl phthalate-mediated oxidative stress induces splenic injury in mice and the attenuating effects of vitamin E and curcumin. Food Chem Toxicol. 2019 Nov 8:110955.  IHC ;  Mouse.   PubMed:31712109 [IF=3.644] Qin X et al. Effects of dietary sea buckthorn pomace supplementation on skeletal muscle mass and meat quality in lambs. Meat Sci . 2020 Aug;166:108141.  WB ;  lamb.   PubMed:32302933 [IF=3.53] Fang C, Zhang J, Qi D, Fan X, Luo J, et al. (2014) Evodiamine Induces G2/M Arrest and Apoptosis via Mitochondrial and Endoplasmic Reticulum Pathways in H446 and H1688 Human Small-Cell Lung Cancer Cells. PLoS ONE 9(12): e115204.  WB ;  Human.   PubMed:25506932 [IF=3.457] Kang K et al. Dexmedetomidine protects against lipopolysaccharide-induced sepsis-associated acute kidney injury via an α7 nAChR-dependent pathway.Biomed Pharmacother. 2018 Oct;106:210-216.  WB ;  Mouse.   PubMed:29960167 [IF=3.23] Daverey, Amita, and Sandeep K. Agrawal. "Curcumin alleviates oxidative stress and mitochondrial dysfunction in astrocytes." Neuroscience 333 (2016): 92-103.  WB ;  Human.   PubMed:27423629 [IF=3.146] Gao, Hui, et al. "Hispidulin induces mitochondrial apoptosis in acute myeloid leukemia cells by targeting extracellular matrix metalloproteinase inducer."American Journal of Translational Research 8.2 (2016): 1115-1132.  WB ;  Human.   PubMed:27158398 [IF=2.914] Xiaoli Gao. et al. Effects of Clostridium perfringens beta2 toxin on apoptosis, inflammation, and barrier function of intestinal porcine epithelial cells. Microb Pathogenesis. 2020 Oct;147:104379  WB ;  Pig.   PubMed:32649964 [IF=2.791] Wang Q et al. Cdc20 and molecular chaperone CCT2 and CCT5 are required for the Muscovy duck reovirus p10. 8-induced cell cycle arrest and apoptosis. Vet Microbiol. 2019 Jun 28;235:151-163.  WB ;  Monkey.   PubMed:31282373 [IF=2.74] Rui Feng. et al. Progesterone regulates inflammation and receptivity of cells via the NF-κB and LIF/STAT3 pathways. THERIOGENOLOGY. Theriogenology. 2022 Jul;186:50  WB ;  Bovine.   PubMed:35430548 [IF=2.44] Tulsulkar, Jatin, et al. "Ginkgo biloba Extract Prevents Female Mice from Ischemic Brain Damage and the Mechanism Is Independent of the HO1/Wnt Pathway." Translational Stroke Research (2015): 1-12.  other ;   PubMed:26573919 [IF=2.37] Akinrinde, A. S., et al. "Alterations in blood pressure, antioxidant status and caspase 8 expression in cobalt chloride-induced cardio-renal dysfunction are reversed by Ocimum gratissimum and gallic acid in Wistar rats." Journal of Trace Elements in Medicine and Biology 36 (2016): 27-37.  Rat.   PubMed:27259349 [IF=2.33] Wu, Chun-Yan, et al. "PCSK9 siRNA Inhibits HUVEC Apoptosis Induced by ox-LDL via Bcl/Bax–Caspase9–Caspase3 Pathway." Molecular and Cellular Biochemistry 359.1-2 (2012): 347-358.  WB ;  Human.   PubMed:21847580 [IF=2.1] Shao S et al. Toosendanin induces apoptosis of MKN‑45 Human gastric cancer cells partly through miR‑23a‑3p‑mediated downregulation of BCL2. Mol Med Rep . 2020 Sep;22(3):1793-1802.  WB ;  Human.   PubMed:32582989 [IF=2.1] Shuli Shao. et al. Toosendanin induces apoptosis of MKN‑45 human gastric cancer cells partly through miR‑23a‑3p‑mediated downregulation of BCL2. Mol Med Rep. 2020 Sep;22(3):1793-1802  WB ;  Human.   PubMed:32582989 [IF=0.939] Pang and Fan Cytotoxic effect and possible mechanisms of Tetracaine on human corneal epithelial cells in vitro. (2016) Int.J.Ophthalmo. 9:497-504  ELISA ;  Human.   PubMed:27162719 [IF=0] Liu F et al. Bioinformatic and experimental findings to indicate anti-cancer targets and mechanisms of calycosin against nasopharyngeal carcinoma. Research Square;12 February 2020.  IF ;  Human&Mouse.   PubMed:doi:10.21203/rs.2.23332/v1
Research Area	Cell biology  Neurobiology  Apoptosis
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Rat, Sheep,  (predicted: Mouse, Chicken, Dog, Pig, Cow, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1μg/Test ICC=1:100 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	12/55kDa
Cellular localization	cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from rat Caspase-8 subunit p10: 411-482/482
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Caspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. Function: Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. Subunit: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 18 kDa (p18) and a 10 kDa (p10) subunit. Interacts with FADD, CFLAR and PEA15. Isoform 9 interacts at the endoplasmic reticulum with a complex containing BCAP31, BAP29, BCL2 and/or BCL2L1. Interacts with TNFAIP8L2. Subcellular Location: Cytoplasm. Tissue Specificity: Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle. DISEASE: Defects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization. Similarity: Belongs to the peptidase C14A family. Contains 2 DED (death effector) domains. SWISS: Q9JHX4 Gene ID: 54474 Database links: Entrez Gene: 841 Human Entrez Gene: 12370 Mouse Entrez Gene: 54474 Rat Entrez Gene: 64044 Rat Omim: 601763 Human SwissProt: Q14790 Human SwissProt: O89110 Mouse SwissProt: Q9JHX4 Rat Unigene: 599762 Human Unigene: 655983 Human Unigene: 336851 Mouse
Product Picture	Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Caspase 8) Polyclonal Antibody, Unconjugated (SL0052R) at 1:500 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: Hela cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Caspase 8) polyclonal Antibody, Unconjugated (SL0052R) 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei. Blank control (blue line): U251 (blue). Primary Antibody (green line): Rabbit Anti-caspase-8 antibody (SL0052R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 70% ethanol overnight at 4℃ and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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