Product Name	ChAT
Chinese Name	ChAT胆碱乙酰转移酶抗体
Alias	Choline O acetyltransferase; Choline O acetyltransferase; Acetyl CoA choline O acetyltransferase; Acetyl CoA:choline O acetyltransferase; ChAT; CHOACTase; Choline acetylase; choline acetyltransferase; CMS1A; CMS1A2; EC 2.3.1.6; OTTHUMP00000019583; OTTHUMP00000019584; CLAT_HUMAN.
literatures	Specific References  (12)     |     SL0042R has been referenced in 12 publications. [IF=5.135] Wei Lu. et al. Effects of targeted muscle reinnervation on spinal cord motor neurons in rats following tibial nerve transection. Neural Regen Res. 2022 Jan;17(8):1827  IHC ;  Rat.   PubMed:35017445 [IF=4.45] Wieck, Minna M., et al. "Human and murine tissue-engineered colon exhibit diverse neuronal subtypes and can be populated by enteric nervous system progenitor cells when donor colon is aganglionic." Tissue Engineering A (2015).  IHC-P ;  Human.   PubMed:26414777 [IF=4.259] Wang,et al.Stimulation of Epicardial Sympathetic Nerves at Different Sites Induces Cardiac Electrical Instability to Various Degrees.(2018) Scientific Reports. 8:994.  IHC-P ;  Dog.   PubMed:29343857 [IF=3.856] Daisuke Koga. et al. Applications of Scanning Electron Microscopy Using Secondary and Backscattered Electron Signals in Neural Structure. Front Neuroanat. 2021; 15: 759804  IF ;  Rat.   PubMed:34955763 [IF=3.842] Chen L et al. Enteric motor dysfunctions in experimental chronic pancreatitis: Alterations of myenteric neurons regulating colonic motility in rats. Neurogastroenterol Motil. 2018 Jul;30(7):e13301.  IF ;  Rat.   PubMed:29520975 [IF=3.58] Zhang, Xiuli, et al. "Catalpol improves cholinergic function and reduces inflammatory cytokines in the senescent mice induced by D-galactose."Food and chemical toxicology 58 (2013): 50-55.  IHC-P ;  Mouse.   PubMed:23612000 [IF=3.536] Zhang,et al.Effects of C1 inhibitor on endothelial cell activation in a rat hind limb ischemia-reperfusion injury model.(2018) Journal of Vascular Surgery. :.  IF(IHC-F) ;  Rat.   PubMed:29395422 [IF=3.53] Zhang Y, Zheng S, Geng Y, Xue J, Wang Z, et al. (2015) MicroRNA Profiling of Atrial Fibrillation in Canines: MiR-206 Modulates Intrinsic Cardiac Autonomic Nerve Remodeling by Regulating SOD1. PLoS ONE 10(3): e0122674.  other ;  Dog.   PubMed:25816284 [IF=3.508] Wieck et al. Human and Murine Tissue-Engineered Colon Exhibit Diverse Neuronal Subtypes and Can Be Populated by Enteric Nervous System Progenitor Cells When Donor Colon Is Aganglionic. (2016) Tissue.Eng.Part.A. 22:53-64  IHC-P ;  Human.   PubMed:26414777 [IF=2.74] Jiasuoer Xiaokereti. et al. Enhanced atrial internal-external neural remodeling facilitates atrial fibrillation in the chronic obstructive sleep apnea model. Plos One. 2021 Feb;16(2):e0247308  WB,IHC ;  Dog.   PubMed:33606818 [IF=2.655] Xiaokereti, Jiasuoer. et al. Renal denervation alleviates chronic obstructive sleep apnea-induced atrial fibrillation via inhibition of atrial fibrosis and sympathetic hyperactivity. SLEEP BREATH. 2023 Feb;:1-14  IF ;  Dog.   PubMed:36811692 [IF=2.014] Li LI. et al. The antibacterial activity of Berberis heteropoda Schrenk and its effect on irritable bowel syndrome in rats. Chin J Nat Medicines. 2020 May;18:356  IHC ;  Rat.   PubMed:10.1016/S1875-5364(20)30042-X
Research Area	Neurobiology  Alzheimer's
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Dog, Pig, )
Applications	ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1ug/Test IF=1:200-800 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	82kDa
Cellular localization	The nucleus cytoplasmic
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human ChAT: 101-200/748
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes an enzyme which catalyzes the biosynthesis of the neurotransmitter acetylcholine. This gene product is a characteristic feature of cholinergic neurons, and changes in these neurons may explain some of the symptoms of Alzheimer's disease. Polymorphisms in this gene have been associated with Alzheimer's disease and mild cognitive impairment. Mutations in this gene are associated with congenital myasthenic syndrome associated with episodic apnea. Multiple transcript variants encoding different isoforms have been found for this gene, and some of these variants have been shown to encode more than one isoform. [provided by RefSeq, May 2010]. Function: Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses. DISEASE: Defects in CHAT are the cause of congenital myasthenic syndrome with episodic apnea (CMSEA) [MIM:254210]; formerly known as familial infantile myasthenia gravis 2 (FIMG2). CMSEA is an autosomal recessive congenital myasthenic syndrome. Patients have myasthenic symptoms since birth or early infancy, negative tests for anti-AChR antibodies, and abrupt episodic crises with increased weakness, bulbar paralysis, and apnea precipitated by undue exertion, fever, or excitement. Similarity: Belongs to the carnitine/choline acetyltransferase family. SWISS: P28329 Gene ID: 1103 Database links: Entrez Gene: 1103 Human Entrez Gene: 12647 Mouse Entrez Gene: 290567 Rat Omim: 118490 Human SwissProt: P28329 Human SwissProt: Q03059 Mouse SwissProt: P32738 Rat Unigene: 302002 Human Unigene: 442817 Mouse Unigene: 45116 Rat 胆碱乙酰转移酶是一种在神经元胞体内合成的酶。当该转移酶被合成以后，通过轴质流动方式转移到神经轴突末端。其功能是将乙酰辅酶A转移到胆碱上，导致神经递质乙酰胆碱的形成。胆碱能系统参与多种神经功能。一些胆碱能神经元的改变能导致阿尔茨海默病的发生。 胆碱乙酰转移酶通常被用来标记神经元。
Product Picture	Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ChAT) Polyclonal Antibody, Unconjugated (SL0042R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ChAT) Polyclonal Antibody, unconjugated (SL0042R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ChAT Polyclonal Antibody, Unconjugated(SL0042R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control:Molt-4. Primary Antibody (green line): Rabbit Anti-ChAT antibody (SL0042R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control (Black line): Molt-4(Black). Primary Antibody (green line): Rabbit Anti-ChAT antibody (SL0042R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed. Blank control: SH-SY5Y. Primary Antibody (green line): Rabbit Anti-ChAT antibody (SL0042R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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