Product Name	AIF
Chinese Name	调亡诱导因子抗体
Alias	Apoptosis inducing factor; Harlequin; Hq; mAIF; MGC111425; MGC5706; PDCD 8; PDCD8; Programmed cell death 8; Programmed cell death 8 isoform 1; Programmed cell death 8 isoform 2; Programmed cell death 8 isoform 3; Programmed cell death protein 8 mitochondrial; Programmed cell death protein 8 mitochondrial precursor; Striatal apoptosis inducing factor; AIFM1_HUMAN; Apoptosis-inducing factor 1, mitochondrial.
literatures	Specific References  (11)     |     SL0037R has been referenced in 11 publications. [IF=4.087]   WB ;  Rat.   PubMed:33300653 [IF=5.4] Gong, Ping, et al. "Hypothermia-induced neuroprotection is associated with reduced mitochondrial membrane permeability in a swine model of cardiac arrest." Journal of Cerebral Blood Flow & Metabolism (2013).  WB ;  Pig.   PubMed:23486294 [IF=4.996] Ji, Wanli. et al. Ischemic stroke protected by ISO-1 inhibition of apoptosis via mitochondrial pathway. SCI REP-UK. 2023 Feb;13(1):1-11  WB,IF ;  Rat.   PubMed:36797398 [IF=4.784] Zhu B et al. The hepatoprotective effect of polysaccharides from Pleurotus ostreatus on carbon tetrachloride-induced acute liver injury rats.Int J Biol Macromol. 2019 Jun 15;131:1-9.  IHF ;  Rat.   PubMed:30851331 [IF=4.556] Chunyue Wang. et al. Isoforsythiaside Attenuates Alzheimer’s Disease via Regulating Mitochondrial Function Through the PI3K/AKT Pathway. Int J Mol Sci. 2020 Jan;21(16):5687  WB ;  Mouse, Human.   PubMed:32784451 [IF=4.087] Zhang Y et al. Gentiopicroside prevents alcoholic liver damage by improving mitochondrial dysfunction in the rat modelPhytother Res.2020 Dec 10.  IHC ;  Rat.   PubMed:33300653 [IF=4.081] Liu, Ke. et al. Effects of Treadmill Exercise on Liver Apoptosis in Fluoride-Exposed Mice. BIOL TRACE ELEM RES. 2023 Mar;:1-13  WB ;  Mouse.   PubMed:36884125 [IF=3.571] Zhang Y et al. Ginsenoside Rg3 prevents cognitive impairment by improving mitochondrial dysfunction in the rat model of Alzheimer's disease. J Agric Food Chem. 2019 Aug 27.  WB&IHC-P ;  Rat.   PubMed:31422666 [IF=3.266] Zhang H et al. Cytoplasmic upregulation of Cyto c and AIF serve as biomarkers of mechanical asphyxia death. Am J Transl Res. 2019 Jul 15;11(7):4568-4583.  WB ;  Rat&Human.   PubMed:31396361 [IF=3.26] Li, Zhiping, et al. "Anti-Oxidative Stress Activity Is Essential for Amanita caesarea Mediated Neuroprotection on Glutamate-Induced Apoptotic HT22 Cells and an Alzheimer’s Disease Mouse Model." International Journal of Molecular Sciences 18.8 (2017): 1623.  WB ;  Mouse.   PubMed:28749416 [IF=1.698] Yan Hu. et al. Microplitis bicoloratus bracovirus regulates cyclophilin A-apoptosis-inducing factor interaction to induce cell apoptosis in the insect immunosuppressive process. 2022 Feb 25  WB,IF ;  Insect（S. Litura）.   PubMed:35218062
Research Area	Tumour  Cell biology  Chromatin and nuclear signals  Neurobiology  Apoptosis  Cyclin  Mitochondrion
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat, Pig,  (predicted: Chicken, Dog, Cow, Rabbit, Sheep, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	57kDa
Cellular localization	The nucleus cytoplasmic Mitochondrion
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human AIF: 131-230/613
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 10. [provided by RefSeq, May 2010]. Function: Probable oxidoreductase that has a dual role in controlling cellular life and death; during apoptosis, it is translocated from the mitochondria to the nucleus to function as a proapoptotic factor in a caspase-independent pathway, while in normal mitochondria, it functions as an antiapoptotic factor via its oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G,and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner. Subunit: Interacts with XIAP/BIRC4. Interacts (via N-terminus) with EIF3G (via C-terminus). Subcellular Location: Mitochondrion intermembrane space. Mitochondrion inner membrane. Cytoplasm. Nucleus. Cytoplasm, perinuclear region. Note=Proteolytic cleavage during or just after translocation into the mitochondrial intermembrane space (IMS) results in the formation of an inner-membrane-anchored mature form (AIFmit). During apoptosis, further proteolytic processing leads to a mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis. Colocalizes with EIF3G in the nucleus and perinuclear region. Tissue Specificity: Isoform 5 is frequently down-regulated in human cancers. Post-translational modifications: Under normal conditions, a 54-residue N-terminal segment is first proteolytically removed during or just after translocation into the mitochondrial intermembrane space (IMS) by the mitochondrial processing peptidase (MPP) to form the inner-membrane-anchored mature form (AIFmit). During apoptosis, it is further proteolytically processed at amino-acid position 101 leading to the generation of the mature form, which is confined to the mitochondrial IMS in a soluble form (AIFsol). AIFsol is released to the cytoplasm in response to specific death signals, and translocated to the nucleus, where it induces nuclear apoptosis in a caspase-independent manner. Ubiquitination by XIAP/BIRC4 does not lead to proteasomal degradation. Ubiquitination at Lys-255 by XIAP/BIRC4 blocks its ability to bind DNA and induce chromatin degradation, thereby inhibiting its ability to induce cell death. DISEASE: Combined oxidative phosphorylation deficiency 6 (COXPD6) [MIM:300816]: A mitochondrial disease resulting in a neurodegenerative disorder characterized by psychomotor delay, hypotonia, areflexia, muscle weakness and wasting. Some patients manifest prenatal ventriculomegaly and severe postnatal encephalomyopathy. Note=The disease is caused by mutations affecting the gene represented in this entry. Similarity: Belongs to the FAD-dependent oxidoreductase family. SWISS: O95831 Gene ID: 9131 Database links: Entrez Gene: 51060 Human Entrez Gene: 9131 Human Entrez Gene: 26926 Mouse Entrez Gene: 83533 Rat Omim: 300169 Human SwissProt: O95831 Human SwissProt: Q9Z0X1 Mouse SwissProt: Q9JM53 Rat AIF是一种易位到The nucleus诱导凋亡的Mitochondrion蛋白, AIF可引起DNA破碎、染色质凝聚，还可诱导细胞色素C和Caspase-9从Mitochondrion中释放出来，AIF从Mitochondrion中的释放可被过度表达的Bcl-2（一种参与Mitochondrion渗透的蛋白质）所抑制。
Product Picture	Sample: Liver (Mouse) Lysate at 40 ug Primary: Anti-AIF (SL 0037R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 63 kD Sample: K562 Cell Lysate at 40 ug Primary: Anti-AIF (SL0037R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 63 kD Sample: Kidney (Mouse) Lysate at 40 ug Primary: Anti-AIF (SL 0037R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 57 kD Observed band size: 63 kD Sample: Lane 1: K562 (Human) Cell Lysate at 30 ug Lane 2: Testis (Rat) Lysate at 40 ug Lane 3: Testis (Mouse) Lysate at 40 ug Primary: Anti-AIF (SL0037R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 67’62’57 kD Observed band size: 62 kD

Cartpieces

• 
• 1

  1Delete

• 
• 5

  5Delete

• 
• S

  SDelete

• 
• R

  RDelete

• 
• +

  +Delete

• 
• 1

  1Delete

• Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 5

  5Delete

• 
• $

  $Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• A

  ADelete

• 
• A

  ADelete

• 

• Delete

• 
• i

  iDelete

• 
• i

  iDelete

• Delete

• 
• 1

  1Delete

• Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• Delete

• 
• 2

  2Delete

• 
• 1

  1Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 1

  1Delete

• 
• 1

  1Delete

• Delete

• 
• -

  -Delete

• 
• -

  -Delete

• 
• 0

  0Delete

• Delete

• 
• t

  tDelete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• 0

  0Delete

• 
• f

  fDelete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• Delete

• 
• 5

  5Delete

• 
• $

  $Delete

• Delete

• 
• 0

  0Delete

• Delete

• Delete

• Delete

• 
• R

  RDelete

• 
• 5

  5Delete

• 
• A

  ADelete

Totalgoods,subtotals:￥Checkout