Product Name	Bcl-2
Chinese Name	Bcl-2抗体
Alias	Apoptosis regulator Bcl 2; Apoptosis regulator Bcl2; AW986256; B cell CLL/lymphoma 2; B cell leukemia/lymphoma 2; B cell lymphoma 2; Bcl 2; Bcl-2; Bcl2; BCL2 protein; C430015F12Rik; D630044D05Rik; D830018M01Rik; Leukemia/lymphoma, B-cell, 2; Oncogene B-cell leukemia 2; BCL2_HUMAN.
literatures	Specific References  (162)     |     SL0032R has been referenced in 162 publications. [IF=18.027] Shikai Liu. et al. On-Demand Generation of Peroxynitrite from an Integrated Two-Dimensional System for Enhanced Tumor Therapy. ACS NANO. 2022;XXXX(XXX):XXX-XXX  WB ;  Human.   PubMed:35666853 [IF=14.593] Guo-Bin Ding. et al. Molecularly engineered tumor acidity-responsive plant toxin gelonin for safe and efficient cancer therapy. Bioact Mater. 2022 Feb;:  IHC ;  Mouse.   PubMed:10.1016/j.bioactmat.2022.02.001 [IF=12.153] Zhang, Fei. et al. Lnc Tmem235 promotes repair of early steroid-induced osteonecrosis of the femoral head by inhibiting hypoxia-induced apoptosis of BMSCs. EXP MOL MED. 2022 Nov;:1-16  WB ;  Rat.   PubMed:36380019 [IF=9.986] Jiyoung Hwang. et al. SOD1 suppresses pro-inflammatory immune responses by protecting against oxidative stress in colitis. Redox Biol. 2020 Oct;37:101760  WB ;  Human.   PubMed:33096425 [IF=8.724] Yonggang Lv. et al. Three-dimensional decellularized tumor extracellular matrices with different stiffness as bioengineered tumor scaffolds. Bioact Mater. 2021 Sep;6:2767  WB ;  Human.   PubMed:33665508 [IF=8.702] Xu Qian. et al. Down-regulation of EVA1A by miR-103a-3p promotes hepatocellular carcinoma cells proliferation and migration. CELL MOL BIOL LETT. 2022 Dec;27(1):1-23  WB ;  Human.   PubMed:36273122 [IF=8.063] Luo J et al. ATF4 destabilizes RET through nonclassical GRP78 inhibition to enhance chemosensitivity to bortezomib in human osteosarcoma. Theranostics. 2019 Aug 14;9(21):6334-6353.  IHC-P ;  Human.   PubMed:31534554 [IF=7.963] Jianzhao Liao. et al. Gut microbiota disturbance exaggerates battery wastewater-induced hepatotoxicity through a gut-liver axis. Sci Total Environ. 2022 Feb;809:152188  WB ;  Mouse.   PubMed:34875328 [IF=7.129] Furui Han. et al. In vivo and in vitro study on hepatotoxicity of Tris-(2, 3-dibromopropyl) isocyanurate exposure via mitochondrial and death receptor pathway. ECOTOX ENVIRON SAFE. 2022 Nov;246:114186  WB ;  Rat, Human.   PubMed:36244175 [IF=7.129] Beiyu Zhang. et al. ZnO-NPs alleviate aflatoxin B1-induced hepatoxicity in ducklings by promoting hepatic metallothionein expression. ECOTOX ENVIRON SAFE. 2023 May;256:114826  WB ;  Duck.   PubMed:36989561 [IF=7.086] Quan-Kuo He. et al. Captan exposure disrupts ovarian homeostasis and affects oocytes quality via mitochondrial dysfunction induced apoptosis. Chemosphere. 2022 Jan;286:131625  WB ;  Mouse.   PubMed:34303901 [IF=6.992] Qingyue Yang. et al. The link between deacetylation and hepatotoxicity induced by exposure to hexavalent chromium. J Adv Res. 2021 Apr;:  WB ;  Rat.   PubMed:10.1016/j.jare.2021.04.002 [IF=6.819] Hongchao Xu. et al. A facile method for anti-cancer drug encapsulation into polymersomes with a core-satellite structure. DRUG DELIV. 2022 Jul 29  IHC ;  Mouse.   PubMed:35904177 [IF=6.792] Jiayi Li. et al. Toxicological effects of deltamethrin on quail cerebrum: Weakened antioxidant defense and enhanced apoptosis. Environ Pollut. 2021 Oct;286:117319  WB ;  Quail.   PubMed:33990053 [IF=6.792] Jiayi Li. et al. Toxicological effects of deltamethrin on quail cerebrum: Weakened antioxidant defense and enhanced apoptosis. Environ Pollut. 2021 Oct;286:117319  WB ;  Quail.   PubMed:33990053 [IF=6.551] Wei J et al. Endosulfan induces cardiotoxicity through apoptosis via unbalance of pro-survival and mitochondrial-mediated apoptotic pathways. Sci Total Environ . 2020 Jul 20;727:138790.  WB ;  human.   PubMed:32344260 [IF=6.543] Zhou Wan. et al. The Effects of RBP4 and Vitamin D on the Proliferation and Migration of Vascular Smooth Muscle Cells via the JAK2/STAT3 Signaling Pathway. Oxid Med Cell Longev. 2022;2022:3046777  WB ;  Rat.   PubMed:35082965 [IF=6.434] Yu D et al. Gestational exposure to acrylamide inhibits mouse placental development in vivo.(2018) J Hazard Mater.367:160-170.  WB ;  Mouse.   PubMed:30594716 [IF=6.388] Weiyi Zhang. et al. Cinnamaldehyde induces apoptosis and enhances anti-colorectal cancer activity via covalent binding to HSPD1. PHYTOTHER RES. 2023 Apr;:  WB ;  Human.   PubMed:37086182 [IF=6.375] Zhou,et al.CXCR4 antagonist AMD3100 enhances the response of MDA-MB-231 triple-negative breast cancer cells to ionizing radiation.(2018) Cancer Letters. 418:196-203.  IHC-P + WB ;  Mouse.   PubMed:29317253 [IF=6.304] Xuemei Shen. et al. CircRILPL1 promotes muscle proliferation and differentiation via binding miR-145 to activate IGF1R/PI3K/AKT pathway. Cell Death Dis. 2021 Feb;12(2):1-14  WB ;  Bovine.   PubMed:33542215 [IF=6.304] Zhiqiang Han. et al. PNO1 regulates autophagy and apoptosis of hepatocellular carcinoma via the MAPK signaling pathway. Cell Death Dis. 2021 May;12(6):1-14  WB ;  Human.   PubMed:34050137 [IF=6.291] Peng Zheng. et al. Alleviative effect of melatonin on the decrease of uterine receptivity caused by blood ammonia through ROS/NF-κB pathway in dairy cow. Ecotox Environ Safe. 2022 Feb;231:113166  WB ;  Bovine.   PubMed:35030520 [IF=6.291] Jia Cui. et al. Effects of ammonia on hypothalamic-pituitary-ovarian axis in female rabbits. Ecotox Environ Safe. 2021 Dec;227:112922  IHC ;  Rabbit.   PubMed:34700170 [IF=6.217] Wang L et al. Zoledronic acid inhibits the growth of cancer stem cell derived from cervical cancer cell by attenuating their stemness phenotype and inducing apoptosis and cell cycle arrest through the Erk1/2 and Akt pathways. J Exp Clin Cancer Res. 2019 Feb 21;38(1):93.  WB ;  Mouse.   PubMed:30791957 [IF=6.064] Mengni Bao. et al. N-Acetylcysteine, an ROS Inhibitor, Alleviates the Pathophysiology of Hyperthyroidism-Induced Cardiomyopathy via the ROS/Ca2+ Pathway. BIOMOLECULES. 2022 Sep;12(9):1195  WB ;  Mouse, Rat.   PubMed:10.3390/biom12091195 [IF=5.763] Xu Kejia. et al. Anti-melanoma effect and action mechanism of a novel chitosan-based composite hydrogel containing hydroxyapatite nanoparticles. REGEN BIOMATER. 2022 Jul;:  IHC ;  Mouse.   PubMed:35958518 [IF=5.682] Fu, Fang. et al. Ndufa4 Regulates the Proliferation and Apoptosis of Neurons via miR-145a-5p/Homer1/Ccnd2. MOL NEUROBIOL. 2023 Feb;:1-18  WB ;  Mouse.   PubMed:36763283 [IF=5.586] Shi-Yi Xu. et al. Long-circulating doxorubicin and Schizandrin A liposome with drug-resistant liver cancer activity: in vitro and in vivo evaluation. J LIPOSOME RES. 2023 Mar 28  IHC ;  Mouse.   PubMed:36974767 [IF=5.201] Xuemei Shen. et al. CircINSR Regulates Fetal Bovine Muscle and Fat Development. Front Cell Dev Biol. 2020; 8: 615638  WB ;  Bovine.   PubMed:33490079
Research Area	Cell biology  Signal transduction  Apoptosis  Cell type markers  TumourCell biologyMaker  The new supersedes the old  Mitochondrion
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,  (predicted: Chicken, Dog, Pig, Cow, Horse, Rabbit, Guinea Pig, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	26kDa
Detection molecular weight	26 kDa
Cellular localization	The nucleus cytoplasmic The cell membrane Mitochondrion
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human Bcl-2: 101-160/236
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	BCL2 is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Two transcript variants (alpha and beta) produced by alternate splicing, differ in their C-terminal ends. BCL2 suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. It regulates cell death by controlling the mitochondrial membrane permeability. It appears to function in a feedback loop system with caspases. BCL2 inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF1). It can form homodimers, and heterodimers with BAX, BAD, BAK and BclX(L). Heterodimerization with BAX requires intact BH1 and BH2 domains, and is necessary for anti-apoptotic activity. Also interacts with APAF1, RAF1, TP53BP2, BBC3, BCL2L1 and BNIPL. Function: Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). Subunit: Forms homodimers, and heterodimers with BAX, BAD, BAK and Bcl-X(L). Heterodimerization with BAX requires intact BH1 and BH2 motifs, and is necessary for anti-apoptotic activity. Interacts with EI24 (By similarity). Also interacts with APAF1, BBC3, BCL2L1, BNIPL, MRPL41 and TP53BP2. Binding to FKBP8 seems to target BCL2 to the mitochondria and probably interferes with the binding of BCL2 to its targets. Interacts with BAG1 in an ATP-dependent manner. Interacts with RAF1 (the 'Ser-338' and 'Ser-339' phosphorylated form). Interacts (via the BH4 domain) with EGLN3; the interaction prevents the formation of the BAX-BCL2 complex and inhibits the anti-apoptotic activity of BCL2. Interacts with G0S2; this interaction also prevents the formation of the anti-apoptotic BAX-BCL2 complex. Subcellular Location: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein. Tissue Specificity: Expressed in a variety of tissues. Post-translational modifications: Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A). Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity. Monoubiquitinated by PARK2, leading to increase its stability. DISEASE: Note=A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions. Similarity: Belongs to the Bcl-2 family. SWISS: P10415 Gene ID: 596 Database links: Entrez Gene: 281020 Cow Entrez Gene: 596 Human Entrez Gene: 12043 Mouse Entrez Gene: 24224 Rat Omim: 151430 Human SwissProt: O02718 Cow SwissProt: P10415 Human SwissProt: P10417 Mouse SwissProt: P49950 Rat Unigene: 150749 Human Unigene: 257460 Mouse Unigene: 9996 Rat Bcl-2基因是指B-cell lymphoma gene。人体滤泡B细胞淋巴瘤中过量表达的原癌基因。由于染色体t（14；18）易位，将Bcl-2基因置于免疫球蛋白重链的转录调控下，使其表达失控。在细胞系中其过量表达能延长细胞存活期而不诱导细胞增殖。它是哺乳动物中细胞调亡的抑制基因。参与Apoptosis的调控。Tumour中的Bcl-2基因可提高侵润性瘤细胞的生存能力。主要用于滤胞型淋巴瘤、毛细管性白血病及Apoptosis等方面的研究。 目前研究认为：Bcl-2也是Apoptosis的一种抑制因子、参与Apoptosis调控，可以用于各种恶性Tumour的Apoptosis的研究。
Product Picture	Protein: Spleen(Mouse)lysate 30ug; Primary: Anti-Bcl-2(SL0032R) at 1:300; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/10000 dilution Predicted band size : 26kD Observed band size : 26kD Protein: Brain(Mouse)lysate 30ug; Primary: Anti-Bcl-2(SL0032R) at 1:200; Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 5000; Predicted band size : 26kD Observed band size : 26kD Sample:Spleen (Mouse) Lysate at 40 ug Primary: Anti-Bcl-2 (SL0032R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 26 kD Sample: Bcl-2 overexpression E.coli lysate (whole cell) Lysate at 40 ug Recombinant protein Lysate at 40 ug Primary: Anti-Bcl-2 (SL0032R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 25 kD Protein: Recombinant protein lysate 100ng; Primary: Anti-Bcl-2(SL0032R) at 1:200; Secondary: HRP conjugated Goat Anti-Rabbit IgG(SL0295G-HRP) at 1: 5000; Predicted band size : 26kD Observed band size : 26kD Sample: Spleen (Mouse) Lysate at 40 ug RAW264.7 Cell (Mouse) Lysate at 40 ug Primary: Anti-Bcl-2 (SL0032R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 26 kD Sample: MCF-7 (Human) Cell Lysate at 30 ug Hela (Human) Cell Lysate at 30 ug HL60 (Human) Cell Lysate at 30 ug A431 (Human) Cell Lysate at 30 ug Primary: Anti-Bcl-2 (SL0032R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 23 kD Sample: Jurkat(Human) Cell Lysate at 30 ug Primary: Anti-Bcl-2 (SL0032R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 26 kD Observed band size: 26 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (SL0032R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (SL0032R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (Rat spinal cord); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (SL0032R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (SL0032R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (SL0032R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human pancreatic cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (SL0032R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (rat ovary tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (SL0032R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: MCF-7 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Bcl-2) Polyclonal Antibody, Unconjugated (SL0032R) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (SL0295G-Cy3) at 37°C for 90 minutes, DAPI (blu Cartpieces 1 1Delete 5 5Delete S SDelete R RDelete + +Delete 1 1Delete Delete Delete 1 1Delete 1 1Delete $ $Delete 0 0Delete 5 5Delete $ $Delete 0 0Delete 0 0Delete 1 1Delete A ADelete A ADelete Delete i iDelete i iDelete Delete 1 1Delete Delete 1 1Delete 1 1Delete 0 0Delete Delete 2 2Delete 1 1Delete 0 0Delete 0 0Delete 0 0Delete 0 0Delete 0 0Delete 0 0Delete 1 1Delete 1 1Delete Delete - -Delete - -Delete 0 0Delete Delete t tDelete 0 0Delete 0 0Delete 0 0Delete f fDelete Delete Delete Delete Delete Delete Delete Delete Delete Delete Delete Delete Delete Delete Delete 5 5Delete $ $Delete Delete 0 0Delete Delete Delete Delete R RDelete 5 5Delete A ADelete Totalgoods,subtotals:￥Checkout Partial purchase records(bought amounts latest0) No one bought this product Total 0 records, divided into1 pages First Prev Next Last User Comment(Total0User Comment Num) No comment Total 0 records, divided into1 pages First Prev Next Last Username： Anonymous user E-mail： Rank： Content： Verification code： Call us +86 571 56623320 Email [email protected] Address Room 1-315, Kongle Changqing Building, No. 160 Guangye Road,Gongshu District, Hangzhou City, Zhejiang Province, China Join Us with Support Share About Us Contact Us Privacy Policy Shipping Policy Payment Policy Quality Guarantee Return & Refund policy Download Product list Leave a message Please select message type Products inquiry (price, lead time, order, etc.) Products inquiry (technical inquiry) Suggestions on website optimization Other suggestions * To protect against spam, please pass the CAPTCHA test below. +86 571 56623320 [email protected] Scan Wechat Qrcode Scan Whatsapp Qrcode