Product Name	Osteopontin
Chinese Name	骨桥蛋白/分泌型磷蛋白1抗体
Alias	BNSP; Bone sialoprotein 1; BSP I; BSPI; Early T lymphocyte activation 1; ETA 1; ETA1; MGC110940; Nephropontin; OPN; osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein; OSTP_HUMAN; secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1); Secreted phosphoprotein 1; SPP 1; SPP-1; SPP1; SPP1/CALPHA1 fusion; Urinary stone protein; Uropontin. OSTP_Mouse.
literatures	Specific References  (28)     |     SL0026R has been referenced in 28 publications. [IF=15.304] Xuan Li. et al. ROS-responsive hydrogel coating modified titanium promotes vascularization and osteointegration of bone defects by orchestrating immunomodulation. BIOMATERIALS. 2022 Aug;287:121683  IHC ;  Rat.   PubMed:35870263 [IF=11.322] Zhenyin Chen. et al. Gelatin/sodium alginate composite hydrogel with dynamic matrix stiffening ability for bone regeneration. COMPOS PART B-ENG. 2022 Aug;243:110162  IHC ;  Rat.   PubMed:10.1016/j.compositesb.2022.110162 [IF=11.092] Fangyu Qiao. et al. Hybrid Cell Membrane-Functionalized Matrixes for Modulating Inflammatory Microenvironment and Improving Bone Defect Repair. ADV HEALTHC MATER. 2023 Apr;:2203047  IHC ;  Rat.   PubMed:37059691 [IF=10.652] Zhuo Liang. et al. Exosome derived from mesenchymal stem cells mediates hypoxia-specific BMP2 gene delivery and enhances bone regeneration. Chem Eng J. 2021 Oct;422:130084  IHC ;  Rabbit.   PubMed:10.1016/j.cej.2021.130084 [IF=8.352] Ye Hea et al. Improved osteointegration by SEW2871-encapsulated multilayers on micro-structured titanium via macrophages recruitment and immunomodulation. Applied Materials Today 20 (2020) 100673  IHC ;  Rat.   PubMed:10.1016/j.apmt.2020.100673 [IF=8.097] Hu Q et al. Demineralized Bone Scaffolds with Tunable Matrix Stiffness for Efficient Bone Integration. ACS Appl Mater Interfaces. 2018 Aug 22;10(33):27669-27680.   IHC-P ;  Rabbit.   PubMed:30063134 [IF=7.6] Wang, Yang, et al. "Induced apoptosis of osteoblasts proliferating on polyhydroxyalkanoates." Biomaterials (2013).  Rat.   PubMed:23433672 [IF=7.59] Zhenyin Chen. et al. Dynamic stiffening collagen-coated substrate enhances osteogenic differentiation of mesenchymal stem cells through integrin α2β1. BIOMATER SCI-UK. 2023 May;:  IF ;  Rat.   PubMed:37233200 [IF=7.328] Dongdong Yao. et al. Matrix stiffness regulates bone repair by modulating 12-lipoxygenase-mediated early inflammation. Mat Sci Eng C-Mater. 2021 Sep;128:112359  IHC ;  Rat.   PubMed:34474906 [IF=6.832] Hou, Yonghui. et al. Nonwoven-based gelatin/polycaprolactone membrane loaded with ERK inhibitor U0126 for treatment of tendon defects. Stem Cell Res Ther. 2022 Dec;13(1):1-11  IHC ;  Rat.   PubMed:35012661 [IF=6.353] Luo Qianting. et al. Dicalcium silicate-induced mitochondrial dysfunction and autophagy-mediated macrophagic inflammation promotes osteogenic differentiation of BMSCs. Regen Biomater. 2021 Dec;:  WB ;  Mouse.   PubMed:10.1093/rb/rbab075 [IF=6.331] Chanjuan Dong. et al. Demineralized and decellularized bone extracellular matrix incorporated electrospun nanofibrous scaffold for bone regeneration. J Mater Chem B. 2021 Aug;:  IHC ;  Rat.   PubMed:10.1039/D1TB00895A [IF=6.208] Samantha Ketelyn Silva. et al. Suitability of Chitosan Scaffolds with Carbon Nanotubes for Bone Defects Treated with Photobiomodulation. INT J MOL SCI. 2022 Jan;23(12):6503  IHC ;  Rat.   PubMed:35742948 [IF=6.183] Wenchao Zhong et al. Dicalcium silicate microparticles modulate the differential expression of circRNAs and mRNAs in BMSCs and promote osteogenesis via circ_1983–miR-6931–Gas7 interaction.. Biomater Sci.2020 Jul 7;8(13):3664-3677.  WB ;  Rat.   PubMed:32463418 [IF=5.88] Xie J et al. The effects of alignment and diameter of electrospun fibers on the cellular behaviors and osteogenesis of BMSCs  IF ;  Human.   PubMed:10.1016/j.msec.2020.111787 [IF=5.88] Qiuping Leng. et al. Demineralized bone matrix scaffold modified with mRNA derived from osteogenically pre-differentiated MSCs improves bone repair. Mat Sci Eng C-Mater. 2021 Feb;119:111601  IHC ;  Rat.   PubMed:33321645 [IF=5.811] Lingli Ding. et al. Ginsenoside Compound K Enhances Fracture Healing via Promoting Osteogenesis and Angiogenesis.. FRONT PHARMACOL. 2022 Apr;13:855393-855393  IF ;  Rat.   PubMed:35462912 [IF=5.763] Cao Yanyan. et al. 3D printed-electrospun PCL/Hydroxyapatite/MWCNTs scaffolds for the repair of subchondral bone. REGEN BIOMATER. 2022 Dec;:  IF ;  Rat.   PubMed:36683741 [IF=4.222] Yi Song. et al. LncRNA SENCR overexpression attenuated the proliferation, migration and phenotypic switching of vascular smooth muscle cells in aortic dissection via the miR-206/myocardin axis. Nutr Metab Cardiovas. 2022 Mar;:  IHC,WB ;  Other.   PubMed:35351345 [IF=4.132] Xu Huang. et al. Osteopontin-Targeted and PPARδ-Agonist-Loaded Nanoparticles Efficiently Reduce Atherosclerosis in Apolipoprotein E–/– Mice. ACS OMEGA. 2022;7(33):28767–28778  WB ;  Mouse.   PubMed:36033674 [IF=3.68] Zhang, Ping, et al. "Contribution of SATB2 to the stronger osteogenic potential of bone marrow stromal cells from craniofacial bones." Cell and Tissue Research 350.3 (2012): 425-437.  WB ;  Rat.   PubMed:22237862 [IF=3.144] Li Jianbin. et al. Characterizations of alveolar repair after mandibular second molar extraction: an experimental study in rats. J APPL ORAL SCI. 2022 Jul;30:  IHC ;  Rat.   PubMed:35830122 [IF=2.885] Lin, Xiaoxin. et al. Shikonin promotes rat periodontal bone defect repair and osteogenic differentiation of BMSCs by p38 MAPK pathway. ODONTOLOGY. 2022 Dec;:1-9  IF ;  Rat.   PubMed:36469160 [IF=2.885] Sheng, Zhenxian. et al. Denervation delays initial bone healing of rat tooth extraction socket. ODONTOLOGY. 2022 Dec;:1-9  IF ;  Rat.   PubMed:36512167 [IF=1.396] Cao LH et al. Irbesartan inhibits the formation of calcium oxalate stones in the kidney of diabetic rats.  WB ;  Rat.   PubMed:ISSN:1940-5901/IJCEM0058003 [IF=0.737] Yang S. Y.. et al. Exosomes Derived from Endothelial Cells Inhibit Neointimal Hyperplasia Induced by Carotid Artery Injury in Rats via ROS-NLRP3 Inflammasome Pathway. B EXP BIOL MED+. 2023 May;:1-6  WB ;  Rat.   PubMed:37162629 [IF=0.513] Komei Kawamoto. et al. A Study of Bone Formation around Titanium Implants Using Frozen Sections. 2021 Apr 15  IHC ;  Rat.   PubMed:10.2485/jhtb.30.165 [IF=0.392] Nakamura S et al. Acceleration of Bone Regeneration of Horizontal Bone Defect in Rats Using Collagen‐Binding Basic Fibroblast Growth Factor Combined with Collagen Scaffolds.J Periodontol. 2019 Mar 19.  IHC-P ;  Rat.   PubMed:30889294
Research Area	Neurobiology  Signal transduction
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse, Rat,
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=2ug/Test IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	34kDa
Cellular localization	Secretory protein
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from mouse OPN: 221-280/294
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	Osteopontin is the principal phosphorylated glycoprotein of bone and is expressed in a limited number of other tissues including dentine. Osteopontin is produced by osteoblasts under stimulation by calcitriol and binds tightly to hydroxyapatite. It is also involved in the anchoring of osteoclasts to the mineral of bone matrix via the vitronectin receptor, which has specificity for osteopontin. Osteopontin is overexpressed in a variety of cancers, including lung, breast, colorectal, stomach, ovarian, melanoma and mesothelioma. Subunit: Ligand for integrin alpha-V/beta-3. Subcellular Location: Secreted. Tissue Specificity: Bone. Found in plasma. Similarity: Belongs to the osteopontin family. SWISS: P10923 Gene ID: 20750 Database links: Entrez Gene: 6696 Human Entrez Gene: 20750 Mouse Entrez Gene: 25353 Rat Omim: 166490 Human SwissProt: P10451 Human SwissProt: P10923 Mouse SwissProt: P08721 Rat Unigene: 313 Human 骨桥蛋白（osteopontin）又称为早期Ｔlymphocyte活化因子Ｉ或分泌型磷蛋白Ｉ,是一种重要的多功能Extracellular matrix蛋白,在骨组织细胞、巨噬细胞、平滑肌细胞、endothelial cells及epithelial cells等多种细胞上表达,在介导细胞的趋化、聚集、黏附、增殖和迁移,以及骨组织的矿化与重建、免疫调节、Signal transduction和对感染性疾病的免疫能力等方面有着重要的作用。 ＯＰＮ在细胞免疫中的功能：1.ＯＰＮ对炎性细胞的作用：炎症是炎性细胞(单核巨噬细胞、lymphocyte、中性粒细胞等)在炎症部位的聚集,适度的炎症对机体有益,如抗感染及促进组织细胞功能恢复等;但过度的炎症能引起组织损伤等有害作用。ＯＰＮ在炎症中具有促炎及抗炎双重作用；2.ＯＰＮ对lymphocyte的作用：早期的报道称ＯＰＮ是Ｔlymphocyte抑制因子,它可抑制Ｔlymphocyte而促进Ｂlymphocyte及肥大细胞的分化、增殖 骨桥蛋白(osteopontin，OPN)是一种含精氨酸—甘氨酸—天冬氨酸(Arg-Gly-Asp，RGD)的分泌型糖基化磷蛋白，已归类于Extracellular matrix(extracellular matrix，ECM)蛋白。 OPN既是cell factor，又是细胞黏附分子。 OPN最初是从骨组织分离的，而后在epithelial cells、胃肠道的分泌物、肾、甲状腺、睾丸以及一些Tumour细胞里都陆续的发现了OPN的表达。在生殖方面，OPN在分泌期子宫内膜epithelial cells、侵袭的滋养细胞、蜕膜细胞中表达。
Product Picture	Sample: Lane 1: HepG2 (Human) Cell Lysate at 30 ug Lane 2: A549 (Human) Cell Lysate at 30 ug Lane 3: U251 (Human) Cell Lysate at 30 ug Primary: Anti-Osteopontin (SL0026R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 34 kD Observed band size: 60 kD Paraformaldehyde-fixed, paraffin embedded (Rat lung); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Osteopontin) Polyclonal Antibody, Unconjugated (SL0026R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining. Tissue/cell: rat shin bone; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-osteopontin Polyclonal Antibody, Unconjugated(SL0026R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-osteopontin Polyclonal Antibody, Unconjugated(SL0026R) 1:300, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Blank control: HepG2. Primary Antibody (green line): Rabbit Anti-Osteopontin antibody (SL0026R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-AF647 Dilution: 1μg /test. Protocol The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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