Product Name	Tyrosine Hydroxylase
Chinese Name	酪氨酸羟化酶抗体
Alias	DYT14; DYT5b; ple; Protein Pale; c; The; TYH; Tyrosine 3 hydroxylase; Tyrosine 3 monooxygenase; Tyk2; TY3H_HUMAN; Tyrosine 3-monooxygenase; Tyrosine 3-hydroxylase; TH.
literatures	Specific References  (10)     |     SL0016R has been referenced in 10 publications. [IF=14.612] Na Zhou. et al. Specific Fluorescent Probe Based on “Protect–Deprotect” To Visualize the Norepinephrine Signaling Pathway and Drug Intervention Tracers. J Am Chem Soc. 2020;142(41):17751–17755  IF ;  Human.   PubMed:33000941 [IF=6.35] Yue Y et al. Noradrenaline-Specific, Efficient Visualization in Brain Tissue Triggered by Unique Cascade Nucleophilic Substitution. Anal Chem. 2019 Feb 5;91(3):2255-2259.  IHF ;  Mouse.   PubMed:30592201 [IF=5.811] Lang Xiu-Yuan. et al. Coeloglossum viride Var. Bracteatum Extract Attenuates MPTP-Induced Neurotoxicity in vivo by Restoring BDNF-TrkB and FGF2-Akt Signaling Axis and Inhibiting RIP1-Driven Inflammation. FRONT PHARMACOL. 2022 Apr;0:1571  WB ;  Mouse.   PubMed:35571135 [IF=4.526] Wen Yi. et al. Suppression of axonal attractant netrin-1 injured dopaminergic neuronal and motor function of mice during manganese over-exposure. METALLOMICS. Metallomics. 2022 Apr;:  WB ;  Mouse.   PubMed:35383848 [IF=2.784] Luo BB et al. Connexin 43 reduces susceptibility to sympathetic atrial fibrillation. INTERNATIONAL JOURNAL OF MOlecular medici. 2018,42(2):1125-1133.  IHC-P ;  Dog.   PubMed:DOI:10.3892/ijmm.2018.3648 [IF=2.761] Ozbey G et al. Metformin protects rotenone-induced dopaminergic neurodegeneration by reducing lipid peroxidation. Pharmacol Rep. 2020 Mar 23.  IHC ;  mouse.   PubMed:32207092 [IF=2.655] Xiaokereti, Jiasuoer. et al. Renal denervation alleviates chronic obstructive sleep apnea-induced atrial fibrillation via inhibition of atrial fibrosis and sympathetic hyperactivity. SLEEP BREATH. 2023 Feb;:1-14  IHC,IF ;  Dog.   PubMed:36811692 [IF=1.966] Tian L et al. Gingerol inhibits cisplatin-induced acute and delayed emesis in rats and minks by regulating the central and peripheral 5-HT, SP, and DA systems. J Nat Med. 2019 Nov 25.  IHC-P ;  rat&mink.   PubMed:31768887 [IF=1.19] Shu et al. Connexin 43 is involved in the sympathetic atrial fibrillation in canine and canine atrial myocytes. (2017) Anatol.J.Cardiol. 18:3-9  IHC ;  Canine.   PubMed:28554986 [IF=1.09] Yu, Yaping, et al. "Overexpression of lactate dehydrogenase-A in human intrahepatic cholangiocarcinoma: its implication for treatment." World Journal of Surgical Oncology 12.1 (2014): 78.  WB ;  Human.   PubMed:24679073
Research Area	Tumour  immunology  Neurobiology  Signal transduction  The new supersedes the old
Immunogen Species	Rabbit
Clonality	Polyclonal
React Species	Human, Mouse,  (predicted: Rat, Dog, Cow, )
Applications	WB=1:500-2000 ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	60kDa
Cellular localization	cytoplasmic The cell membrane
Form	Liquid
Concentration	1mg/ml
immunogen	KLH conjugated synthetic peptide derived from human TH: 101-165/528
Lsotype	IgG
Purification	affinity purified by Protein A
Buffer Solution	0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage	Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is involved in the conversion of tyrosine to dopamine. It is the rate-limiting enzyme in the synthesis of catecholamines, hence plays a key role in the physiology of adrenergic neurons. Mutations in this gene have been associated with autosomal recessive Segawa syndrome. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jul 2008] Function: Plays an important role in the physiology of adrenergic neurons. Tissue Specificity: Mainly expressed in the brain and adrenal glands. DISEASE: Defects in TH are the cause of Segawa syndrome autosomal recessive (ARSEGS) [MIM:605407]. A form of DOPA-responsive dystonia presenting in infancy or early childhood. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Some cases present with parkinsonian symptoms in infancy. Unlike all other forms of dystonia, it is an eminently treatable condition, due to a favorable response to L-DOPA. Note=May play a role in the pathogenesis of Parkinson disease (PD). A genome-wide copy number variation analysis has identified a 34 kilobase deletion over the TH gene in a PD patient but not in any controls. Similarity: Belongs to the biopterin-dependent aromatic amino acid hydroxylase family. SWISS: P07101 Gene ID: 7054 Database links: Entrez Gene: 7054 Human Entrez Gene: 21823 Mouse Entrez Gene: 25085 Rat Omim: 191290 Human SwissProt: P07101 Human SwissProt: P24529 Mouse SwissProt: P04177 Rat Unigene: 435609 Human Unigene: 1292 Mouse Unigene: 11082 Rat 神经细胞Maker 酪氨酸羟化酶（TH）是儿茶酚胺类神经递质即多巴胺、去甲肾上腺素、肾上腺素生物合成过程所需的限速酶，它以四氢生物喋呤啶(BH4)为辅酶，催化酪氨酸的羟化而生成多巴(DOPA)。 已知在患帕金森病(Parkinson disease,PD)时，脑内多巴胺(dopamine,DA)的减少与此酶活性低下有关。因此对PD模型动物来说，若将TH基因植入脑内，便可以提高脑内DA水平而达到基因治疗目的。
Product Picture	Sample: adipocyte (mouse) Lysate at 5-10 ug model A, model B, model C are from different mice; Primary: Anti-Tyrosine Hydroxylase(SL0016R) at 1/2000 dilution Predicted band size: 60 kD Observed band size: 60 kD Sample: PC-3 (Mouse) Lysate at 30 ug Primary: Anti-Tyrosine Hydroxylase (SL0016R) at 1:300 dilution; Secondary: HRP conjugated Goat-Anti-rabbit IgG(SL0295G-HRP) at 1:5000 dilution; Predicted band size:60 kD Observed band size:60 kD Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (SL0016R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (SL0016R ) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining. Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Tyrosine Hydroxylase Polyclonal Antibody, Unconjugated(SL0016R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (SL0016R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (SL0295G-FITC) for 90 minutes, and DAPI for nuclei staining. Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Tyrosine Hydroxylase) Polyclonal Antibody, Unconjugated (SL0016R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (SL0295G-FITC) for 90 minutes, and DAPI for nuclei staining.

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