Product Name	Cytokeratin 15(ready to use)
Chinese Name	细胞角蛋白15单克隆抗体（工作液）
Research Area	Tumour  Cell biology  immunology  Signal transduction  Cytoskeleton
Immunogen Species	Mouse
Clonality	Monoclonal
Clone NO.	3H5
React Species	Human,
Applications	IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （Paraffin sections need antigen repair） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
Theoretical molecular weight	49kDa
Cellular localization	The nucleus cytoplasmic The cell membrane
Form	Liquid
immunogen	KLH conjugated synthetic peptide derived from human Cytokeratin 15
Lsotype	IgG1
Purification	affinity purified by Protein G
Buffer Solution	0.01M PBS(pH7.4) with 1% BSA and 0.1% Proclin300
Storage	Store at 2-8 °C for one year. Avoid repeated freeze/thaw cycles.
Attention	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed	PubMed
Product Detail	The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains and are clustered in a region on chromosome 17q21.2. [provided by RefSeq]. Subunit: Heterotetramer of two type I and two type II keratins. Subcellular Location: Expressed in a discontinuous manner in the basal cell layer of adult skin epidermis, but continuously in the basal layer of fetal skin epidermis and nail. Also expressed in the outer root sheath above the hair bulb in hair follicle (at protein level). Expressed homogeneously in all cell layers of the esophagus and exocervix, but detected in the basal cell layer only of oral mucosa, skin and in the basal plus the next two layers of the suprabasal epithelium of the palate. Similarity: Belongs to the intermediate filament family. SWISS: P19012 Gene ID: 3866 Database links: Entrez Gene: 3866 Human Entrez Gene: 16665 Mouse Entrez Gene: 287700 Rat Omim: 148030 Human SwissProt: P19012 Human SwissProt: Q61414 Mouse SwissProt: Q6IFV3 Rat Unigene: 654570 Human Unigene: 440010 Mouse Unigene: 153568 Rat
Product Picture	Paraformaldehyde-fixed, paraffin embedded (human esophagus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 15) Monoclonal Antibody, Unconjugated (BH0201) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining. Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cytokeratin 15) Monoclonal Antibody, Unconjugated (BH0201) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Mouse)(sp-0024) instructionsand DAB staining.

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