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Rabbit Anti-MRPL30/RBITC Conjugated antibody
Rabbit Anti-MRPL30/RBITC Conjugated antibody
CCDC113; 39S ribosomal protein L28, mitochondrial; 39S ribosomal protein L30, mitochondrial; Coiled-coil domain-containing protein 113 Gene names; DKFZp434N1418; HSPC065; L28MT; L30MT; Mitochondrial ribosomal protein L30; MRP L28; MRP L30; MRPL28; MRPL28M
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Product Name Anti-MRPL30/RBITC
Chinese Name 罗丹明(RBITC)标记的Mitochondrion核糖体蛋白L30抗体
Alias CCDC113; 39S ribosomal protein L28, mitochondrial; 39S ribosomal protein L30, mitochondrial; Coiled-coil domain-containing protein 113 Gene names; DKFZp434N1418; HSPC065; L28MT; L30MT; Mitochondrial ribosomal protein L30; MRP L28; MRP L30; MRPL28; MRPL28M; RPML28.  
Research Area Cell biology  immunology  Chromatin and nuclear signals  transcriptional regulatory factor  Cell type markers  Mitochondrion  Epigenetics  
Immunogen Species Rabbit
Clonality Polyclonal
React Species (predicted: Human, Mouse, Rat, )
Applications ICC=1:50-200 IF=1:50-200 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
Molecular weight 16kDa
Form Lyophilized or Liquid
Concentration 1mg/ml
immunogen KLH conjugated synthetic peptide derived from human MRPL30
Lsotype IgG
Purification affinity purified by Protein A
Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Product Detail background:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the EcoL2 ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 12q. [provided by RefSeq, Jul 2008]

Function:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Alternative splicing results in multiple transcript variants. Pseudogenes corresponding to this gene are found on chromosomes 6p and 12p. Read-through transcription also exists between this gene and the neighboring upstream lipoyltransferase 1 (LIPT1) gene.

Subcellular Location:
Mitochondrion

Similarity:
Belongs to the ribosomal protein L30P family.

Database links:

Entrez Gene: 474558 Dog

Entrez Gene: 51263 Human

Entrez Gene: 107734 Mouse

Entrez Gene: 301352 Rat

Omim: 611838 Human

SwissProt: Q8TCC3 Human

SwissProt: Q9D7N6 Mouse

SwissProt: P0C2C1 Rat



Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

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