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Product Name Anti-phospho-MAX protein(Tyr123)/RBITC Chinese Name 罗丹明(RBITC)标记的磷酸化Myc基因相关X因子抗体 Alias bHLHd4; bHLHd5; bHLHd6; bHLHd7; bHLHd8; Class D basic helix-loop-helix protein 4; Helix loop helix zipper protein; MAX protein; MGC10775; MGC11225; MGC18164; MGC34679; MGC36767; Myc associated factor X; Myc-associated factor X; MAX_HUMAN; Myc binding novel HLH/LZ protein; Orf 1; Orf1; Protein max. Product Type Phosphorylated anti Research Area Tumour Cell biology immunology Chromatin and nuclear signals Signal transduction Epigenetics Immunogen Species Rabbit Clonality Polyclonal React Species (predicted: Human, Mouse, Rat, Horse, ) Applications IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 18kDa Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human MAX protein around the phosphorylation site of Tyr123 Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Product Detail background:
MAX protein is a member of the basic helix-loop-helix leucine zipper (bHLHZ) family of transcription factors. It is able to form homodimers and heterodimers with other family members, which include Mad, Mxi1 and Myc. Myc is an oncoprotein implicated in cell proliferation, differentiation and apoptosis. The homodimers and heterodimers compete for a common DNA target site (the E box) and rearrangement among these dimer forms provides a complex system of transcriptional regulation. Mutations of this gene have been reported to be associated with hereditary pheochromocytoma. A pseudogene of this gene is located on the long arm of chromosome 7. Alternative splicing results in multiple transcript variants.
Function:
Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC-MAX complex is a transcriptional activator, whereas the MAD-MAX complex is a repressor. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity.
Subunit:
Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MYC or MAD. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EUHMTASE1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Component of some MLL1/MLL complex, at least composed of the core components MLL, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, KAT8/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with SPAG9.
Subcellular Location:
Nucleus.
Tissue Specificity:
High levels found in the brain, heart and lung while lower levels are seen in the liver, kidney and skeletal muscle.
Post-translational modifications:
Reversible lysine acetylation might regulate the nuclear-cytoplasmic shuttling of specific Max complexes.
Similarity:
Belongs to the MAX family.
Contains 1 bHLH (basic helix-loop-helix) domain.
Database links:Entrez Gene: 4149 Human
Entrez Gene: 17187 Mouse
Omim: 154950 Human
SwissProt: P52162 Chicken
SwissProt: P61244 Human
SwissProt: P28574 Mouse
Unigene: 285354 Human
Unigene: 4210 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
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