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Product Name Anti-Phospho-RhoA (Ser188)/PE Chinese Name PE标记的磷酸化RhoA蛋白抗体 Alias RHOA(phospho S188); RhoA (phospho S188); RhoA protein; Aplysia ras related homolog 12; ARH 12; ARH12; ARHA; H 12; Oncogene RHO H12; Ras homolog gene family member A; RHO 12; Rho A; RHO H12; RHO12; RHOH 12; RHOH12; Small GTP binding protein Rho A; Small GTP binding protein RhoA; Transforming protein Rho A; Transforming protein RhoA. Product Type Phosphorylated anti Research Area immunology Signal transduction Apoptosis Kinases and Phosphatases Immunogen Species Rabbit Clonality Polyclonal React Species Human, Rat, (predicted: Mouse, Chicken, Dog, Cow, Sheep, ) Applications Flow-Cyt=1:50-200 IF=1:50-200
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.Molecular weight 21kDa Form Lyophilized or Liquid Concentration 1mg/ml immunogen KLH conjugated Synthesised phosphopeptide derived from human RhoA around the phosphorylation site of Ser188 Lsotype IgG Purification affinity purified by Protein A Storage Buffer 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Storage Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. Product Detail background:
This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified.
Subunit:
nteracts with RGNEF. Binds PRKCL1, ROCK1 and ROCK2. Interacts with ARHGEF2, ARHGEF3, NET1 and RTKN. Interacts with PLCE1 and AKAP13. Interacts (in the constitutively activated, GTP-bound form) with DGKQ. Interacts with human respiratory syncytial virus (HRSV) protein F; this interaction facilitates virus-induced syncytium formation. Interacts with GNB2L1/RACK1; enhances RHOA activation. Interacts with PKP4; the interaction is detected at the midbody. Interacts (GTP-bound form preferentially) with PKN2; the interaction stimulates autophosphorylation and phosphorylation of PKN2.
Similarity:
Belongs to the small GTPase superfamily. Rho family.
Database links:Entrez Gene: 387 Human
Entrez Gene: 11848 Mouse
Omim: 165390 Human
SwissProt: P61586 Human
SwissProt: Q9QUI0 Mouse
Unigene: 247077 Human
Unigene: 757 Mouse
Unigene: 107401 Rat
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
RhoA蛋白主要分布在细胞质和The cell membrane,RhoA蛋白家族是控制Cytoskeleton蛋白组合、调节基因表达等细胞活动过程中的关键Signal transduction成分。通常,RhoA通过激活其下游的Rho 连接激酶(Rho associated kinase,ROK)而起作用。在调节Cytoskeleton组建的过程中,RhoA与其作用对象均在胞质内。
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